Primary structure of a lipoxygenase from barley grain as deduced from its cDNA sequence

article






Mechelen, J.R. van

Smits, M.

Douma, A.C.

Rouster, J.

Cameron-Mills, V.

Heidekamp, F.

Valk, B.E.
A full length cDNA sequence for a barley grain lipoxygenase was obtained. It includes a 5' untranslated region of 69 nucleotides, an open reading frame of 2586 nucleotides encoding a protein of 862 amino acid residues and a 3' untranslated region of 142 nucleotides. The molecular mass of the encoded polypeptide was calculated to be 96.392. Its amino acid sequence shows a high homology with that of other plant lipoxygenases identified to date.
Topics




















BarleyCDNA cloningHordeum vulgare L.Inverse PCR cloningLOXPlant lipoxygenaseLipoxygenaseBarleyDna sequenceEnzyme analysisPhytochemistryPriority journalProtein analysisAmino Acid SequenceBase SequenceDNA, ComplementaryHordeumLipoxygenaseMolecular Sequence DataPolymerase Chain ReactionSequence Analysis
TNO Identifier
232921
Repository link
https://resolver.tno.nl/uuid:d40b0284-e368-4f86-9045-d9ea532b0fd9
ISSN
00052760
Source
Biochimica et Biophysica Acta - Lipids and Lipid Metabolism, 1254(2), pp. 221-225.
Pages
221-225
Files
To receive the publication files, please send an e-mail request to TNO Repository.