B-type granule containing protrusions and interconnections between amyloplasts in developing wheat endosperm revealed by transmission electron microscopy and GFP expression

article




Langeveld, S.M.J.

Wijk, R. van

Stuurman, N.

Kijne, J.W.

Pater, S. de
Starch granules in mature wheat endosperm show a bimodal size distribution. The formation of small starch granules in wheat endosperm cells was studied by transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) after expression and targeting of fluorescent protein into amyloplasts. Both techniques demonstrated the presence of protrusions emanating from A-type granules-containing amyloplasts and the presence of B-type starch granules in these evaginations. Moreover, CLSM recordings demonstrated the interconnection of the amyloplasts by these protrusions, suggesting a possible role of these protrusions in interplastid communication.
Topics

























AmyloplastsB-type starch granulesCLSMProtrusionsWheat endospermGreen fluorescent proteinPhotoproteinPrimer DNACell organelleConfocal microscopyElectron microscopyGeneticsGrowth, development and agingMetabolismNucleotide sequenceUltrastructureWheatBase SequenceDNA PrimersGreen Fluorescent ProteinsLuminescent ProteinsMicroscopy, ConfocalMicroscopy, ElectronOrganellesTriticumTriticum aestivum
TNO Identifier
87337
Repository link
https://resolver.tno.nl/uuid:de0155f6-61f9-47fe-97ce-a9eae33c33be
Source
Journal of Experimental Botany, 51, pp. 1357-1361.
Pages
1357-1361
Files
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