Intercellular communication and cell proliferation in precision-cut rat liver slices : effect of medium composition and DDT

article



Graaf, I.A.M.

Tajima, O.

Groten, J.P.

Wolterbeek, A.P.M.
Gap junctional intercellular communication (GJIC) and cell proliferation were studied in control and 1,1'-bis(p-chlorophenyl)-2,2,2,-trichloroethane (DDT) treated precision-cut liver slices of rat by evaluating connexin 32 (Cx32) expression and 5-bromo-2'-deoxyuridine (BrdU) incorporation. In addition, the effect of different culture media (RPMI and WME) on control and DDT influenced Cx32 expression and cell proliferation was determined. Cx32 expression in control precision-cut liver slices was maintained during 8 h of culturing, but decreased after prolonged culturing. Control cell proliferation was significantly higher when WME was used as culture medium than when RPMI was used. In slices treated with DDT Cx32 expression was decreased. In slices cultured in RPMI medium, this decrease preceded a dose-dependent increase in cell proliferation. These results show the usefulness of precision-cut liver slices in studying cellular proliferation and intercellular communication. Copyright (C) 2000 Published by Elsevier Science Ireland Ltd.
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1,1'-Bis(p-chlorophenyl)-2,2,2,-trichloroethane3-Aminopropyltriethoxysilane5-Bromo-2'-deoxyuridineCell proliferationConnexin 32DiaminebenzidineDimethyl sulfoxideFetal bovine serumGap junctional intercellular communicationHistomorphologyIntercellular communicationLactate dehydrogenasePhosphate-buffered salinePrecision-cut liver slicesRPMI 1640 mediumWilliams medium EBroxuridineChlorphenotaneConnexin 32Dimethyl sulfoxideLactate dehydrogenaseTissue culture mediumAnimal experimentAnimal modelAnimal tissueCarcinogenesisCell communicationCell proliferationControlled studyGap junctionImmunocytochemistryLiver sliceMaleMorphometricsNonhumanPriority journalRatAnimalsBlotting, WesternBromodeoxyuridineCell DivisionCells, CulturedConnexinsCulture MediaDDTDose-Response Relationship, DrugGap JunctionsImmunohistochemistryL-Lactate DehydrogenaseLiverMaleRatsRats, WistarSignal TransductionTime FactorsBovinae
TNO Identifier
87150
Repository link
https://resolver.tno.nl/uuid:e2c47453-41a8-4edf-9e14-dc3581144501
ISSN
03043835
Source
Cancer Letters, 154(1), pp. 53-62.
Pages
53-62
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