Comparison between collision induced dissociation of electrosprayed protonated peptides in the up-front source region and in a low-energy collision cell
article
A systematic comparison between the up-front Collision induced dissociation (CID) mass spectra and low-energy CID tandem mass spectra from twenty-one singly and/or doubly charged peptides has been made. CID spectra of the peptides were recorded at different electrode voltages in the up-front source region of a single quadrupole instrument and different collision energies in the collision cell of a tandem quadrupole instrument. It was observed that up-front CID and low-energy CID yielded comparable product ion spectra from protonated peptides, and that the instrumental settings necessary for obtaining comparable CID mass spectra from the two methods are correlated.Chemicals/CAS: Peptide Fragments; Peptides
Topics
TNO Identifier
86930
ISSN
09514198
Source
Rapid Communications in Mass Spectrometry, 13(17), pp. 1712-1716.
Pages
1712-1716
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