Selection of Arabidopsis mutants overexpressing genes driven by the promoter of an auxin-inducible glutathione S-transferase gene
article
Transgenic arabidopsis plants were isolated that contained a T-DNA construct in which the promoter of an auxin-inducible glutathione S-transferase (GST) gene from tobacco was fused to the kanamycin resistance (nptII) as well as to the β-glucuronidase (gusA) reporter gene. Subsequently, seeds were treated with EMS to obtain mutants in which both reporter gene fusions were up-regulated. Northern analysis showed that the mRNA level of a related, endogenous auxin-inducible GST gene of Arabidopsis was increased in some of these mutants as well. Two of the gup (GST up-regulated) mutants were characterized in more detail and roughly mapped. Both had epinastic cotyledons and leaves, a phenotype that turned out to be linked to the gup mutation. Chemicals/CAS: DNA, Bacterial; Ethyl Methanesulfonate, 62-50-0; Glutathione Transferase, EC 2.5.1.18; Indoleacetic Acids; Mutagens; Recombinant Fusion Proteins; T-DNA
Topics
Arabidopsis thalianaAuxin-responsive gene expressionDevelopmental mutantsGlutathione S-transferaseGSTGup mutantAuxinBeta glucuronidaseGene expressionGlutathione transferaseKanamycinReporter geneTransgenic plantArabidopsisChromosome MappingCrosses, GeneticDNA, BacterialEnzyme InductionEthyl MethanesulfonateGene Expression Regulation, PlantGenes, PlantGlutathione TransferaseIndoleacetic AcidsMutagenesisMutagensMutationPhenotypePlants, Genetically ModifiedPromoter Regions (Genetics)Recombinant Fusion ProteinsUp-Regulation
TNO Identifier
86824
ISSN
01674412
Source
Plant Molecular Biology, 39(5), pp. 979-990.
Pages
979-990
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