Construction and heterologous expression of a synthetic copy of the cutinase cDNA from Fusarium solani pisi

article



Gemeren, I.A. van

Musters, W.

Hondel, C.A.M.J.J. van den

Verrips, C.T.
A copy of the cutinase cDNA from Fusarium solani pisi was constructed starting from synthetic oligonucleotides. For this construction three separate cassettes were made, which were subsequently assembled to form the cutinase gene. Heterologous expression of the synthetic cutinase gene and the subsequent secretion of the recombinant enzyme was achieved in Saccharomyces cerevisiae and Aspergillus awamori.
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A. awamoriF. solani pisi cutinaseFilamentous fungiHeterologous expressionS. cerevisiaeSynthetic gene constructionComplementary dnaCutinaseAspergillusBiosynthesisControlled studyFusarium solaniGene expressionNonhumanPriority journalSaccharomyces cerevisiaeAmino Acid SequenceAspergillusBase SequenceBiotechnologyCarboxylic Ester HydrolasesDNA, ComplementaryDNA, FungalFusariumGenes, FungalGenes, SyntheticGenetic VectorsMolecular Sequence DataPlasmidsRecombinant ProteinsSaccharomyces cerevisiaeSupport, Non-U.S. Gov'tAspergillusAspergillus awamoriFungiFusariumFusarium solaniSaccharomyces cerevisiae
TNO Identifier
83418
Repository link
https://resolver.tno.nl/uuid:91d8e5c4-e538-42f8-b106-1dfe3695197d
Source
Journal of Biotechnology, 40, pp. 155-162.
Pages
155-162
Files
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