The thymus atrophy inducing organotin compound DBTC stimulates TCRalfabeta-CD3 signalling in immature rat thymocytes

article





Pieters, R.H.H.

Punt, P.

Bol, M.

Dijken, J.M. van

Seinen, W.

Penninks, A.H.
In the present study, we show that the thymus atrophy inducing compound DBTC stimulates the intracellular release, but not the influx, of Ca2+ elicited by cross-linking of the TcRαβ-CD3-complex on rat thymocytes and inhibits capping of TcRαβ. Similarities with the effects of cytochalasin B together with the finding that DBTC also inhibited capping of CD8, whereas cross-linking of CD8 did not cause a Ca2+-response, suggest that DBTC interferes with TcRαβ-CD3-signalling by selective interference with cytoskeletal functioning. The responding thymocytes were CD53- and FSC(low), thus possibly including the non proliferating counterpart of the presumed immature CD4-CD8+CD53- target cells of DBTC. The present effects may therefore relate to the mechanisms of organotin-induced thymus atrophy.
Chemicals/CAS: Azides; Calcium, 7440-70-2; Cytochalasin B, 14930-96-2; dibutyldichlorotin, 683-18-1; Organotin Compounds; Receptor-CD3 Complex, Antigen, T-Cell; Receptors, Antigen, T-Cell, alpha-beta; Sodium Azide, 26628-22-8; Teratogens
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AnimalAzidesCalciumCells, CulturedComparative StudyCytochalasin BKineticsMaleOrganotin CompoundsRatsRats, WistarReceptor-CD3 Complex, Antigen, T-CellReceptors, Antigen, T-Cell, alpha-betaSignal TransductionSodium AzideSupport, Non-U.S. Gov'tT-LymphocytesTeratogensThymus GlandAnimalia
TNO Identifier
82719
Repository link
https://resolver.tno.nl/uuid:bb745af7-2298-4849-892a-b3a51b771c06
ISSN
0006291X
Source
Biochemical and Biophysical Research Communications, 214(2), pp. 552-558.
Pages
552-558
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