Fourier transform infrared spectroscopy with a sample deposition interface as a quantitative detector in size-exclusion chromatography
article
The use of a state-of-the-art commercial solvent-elimination interface for liquid chromatography-infrared spectroscopy is discussed from the perspective of quantitative analysis. The effect of eluent flow-rate is investigated with respect to the homogeneity of the deposit and the trace width along the deposition trace. Low flow-rates (50 μl/min or less) turn out to be favorable for obtaining a good trace and a high sensitivity. The trace width decreased from 2.20 to 1.13 mm when the flow-rate was reduced from 500 to 25 μl/min. Preservation of chromatographic detail during deposition was evaluated at different substrate moving speeds. The additional (extra-column) band broadening that is inherent to nebulizer-deposition interfaces, causes a loss in resolution in size-exclusion chromatography (SEC)-Fourier transform infrared (FTIR) spectroscopy in comparison with SEC-UV. The repeatability of the deposition is evaluated by SEC-FTIR analyses of polystyrene standards (peak molar-masses: 1250-2 950 000 g/mol) and the RSD is found to range from 3.2 to 5.8% in response and from 0.21 to 0.47% in retention time. © 2002 Elsevier Science B.V. All rights reserved. Chemicals/CAS: Indicators and Reagents; Polystyrenes; Solvents
Topics
TNO Identifier
72665
ISSN
00219673
Source
Journal of Chromatography A,, 948(1-2), pp. 257-265.
Pages
257-265
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