An expression system based on the promoter region of the Aspergillus awamori 1,4-bèta-endoxylanase A gene
article
A new, highly inducible fungal promoter derived from the Aspergillus awamori 1,4-β-endoxylanase A (exlA) gene is described. Induction analysis, carried out with the wild-type strain in shake flasks, showed that exlA expression is regulated at the transcriptional level. Using a β-glucuronidase (uidA) reporter strategy, D-xylose was shown to be an efficient inducer of the exlA promoter, whereas sucrose or maltodextrin were not. Upon D-xylose induction, the exlA promoter was threefold more efficient than the frequently used A. niger glucoamylase (glaA) promoter under maltodextrin induction. Detailed induction analyses demonstrated that induction was-dependent on the presence of D-xylose in the medium. Carbon-source-limited chemostat cultures with the uidA reporter strain showed that D-xylose was also a very good inducer in a fermenter, even in the presence of sucrose.
Topics
EnzymeGlycosidaseMicrobial enzymeControlled studyGene expression regulationMolecular cloningNonhumanPromoter regionAspergillusEndo-1,4-beta XylanasesEnzyme InductionGene Expression Regulation, FungalGenes, ReporterGenetic VectorsGlucan 1,4-alpha-GlucosidaseGlucuronidasePromoter Regions (Genetics)RNA, FungalRNA, MessengerTranscription, GeneticXyloseXylosidasesAspergillus awamoriAspergillus nigerTrixis
TNO Identifier
68889
Source
Appied Microbiology and Biotechnology, 46, pp. 28-35.
Pages
28-35
Files
To receive the publication files, please send an e-mail request to TNO Repository.