Molecular cloning and functional expression in Lactobacillus plantarum 80 of xylT, encoding the D-xylose-H+ symporter of Lactobacillus brevis

A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding D-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could code for the D-xylulose kinase gene (xylB) and another gene (xylT) encoding a protein of 457 amino acids with significant similarity to the D-xylose-H...

Intestinal beta-carotene absorption and cleavage in men: Response of beta-carotene and retinyl esters in the triglyceride-rich lipoprotein fraction after a single oral dose of beta-carotene

Postprandial response curves of β-carotene and retinyl esters in a triglyceride-rich lipoprotein (TRL) fraction were evaluated as a potential measure of β-carotene uptake and cleavage. β-Carotene, retinyl ester, and triglyceride concentrations in the TRI, fraction (density < 1.006 kg/L) and plasma were measured in 10 men for 8 or 16 h after an...

Ethnicity and maternal education as risk indicators for dental caries, and the role of dental behavior.

The aim of this study was to assess differences in dental health between socioeconomic and ethnic groups in a cohort of 5-yr-old children born in 1982 and in a cohort of 11-yr-old children born in 1976. A further aim was to evaluate the putative role of dental behaviors as intervening factors between ethnicity and maternal education on the one...

The digestion process of the sugar alcohol isomalt in the intestinal tract of the pig: 1 Studies with administration of isomalt in the feed

In a study with twelve pigs of 60-70 kg live weight provided with a re-entrant cannula at the end of the ileum, and twelve intact, non-cannulated pigs, the fate of dietary doses of 100 and 200 g isomalt/kg during gastrointestinal passage was examined. From sugar analyses in ileal chyme it was calculated that 0.43 and 0.30 of the isomalt consumed...

The digestion process of the sugar alcohol isomalt in the intestinal tract of the pig: 2 Studies with administration of isomalt as a sweet

In a study with ten pigs of 60-70 kg live weight, provided with a re-entrant cannula at the end of the ileum, and sixteen intact, non-cannulated pigs, the digestion and absorption of a dietary dose of 100 g isomalt/kg, and isomalt given between the meals as a 'sweet' on the basis of 50 and 100 g/kg feed consumption, were examined. In all three...

Mutational analysis of pea lectin: Substitution of Asn125 for Asp in the monosaccharide-binding site eliminates mannose/glucose-binding activity

As part of a strategy to determine the precise role of pea (Pisum sativum) lectin, Psl, in nodulation of pea by Rhizobium leguminosarum, mutations were introduced into the genetic determinant for pea lectin by site-directed mutagenesis using PCR. Introduction of a specific mutation, N125D, into a central area of the sugar-binding site resulted...

Evaluation of histological changes in the kidneys of the alloxan diabetic rat by means of factor analysis

Renal histopathology data, obtained in a 3-month feeding study of the effects of different carbohydrates and polyols in the alloxan diabetic rat, have been subjected to factor analysis. The results indicate a primary and a secondary effect of the diabetic state on the kidney of the rat. The primary effect, the 'diabetic factor', was associated...