Searched for: subject%3A%22protein%255C%2Bfolding%22
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van Noort, J.M. (author), TNO Kwaliteit van Leven (author)
Stress proteins or heat shock proteins (HSPs) are ubiquitous cellular components that have long been known to act as molecular chaperones. By assisting proper folding and transport of proteins, and by assisting in the degradation of aberrant proteins, they play key roles in cellular metabolism. The frequent accumulation of insoluble protein...
article 2008
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TNO Kwaliteit van Leven (author), Kort, R. (author), Keijser, B.J. (author), Caspers, M.P.M. (author), Schuren, F.H. (author), Montijn, R. (author)
Background: In bacteriology, the ability to grow in selective media and to form colonies on nutrient agar plates is routinely used as a retrospective criterion for the detection of living bacteria. However, the utilization of indicators for bacterial viability-such as the presence of specific transcripts or membrane integrity-would overcome bias...
article 2008
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Broersen, K. (author), Weijers, M. (author), de Groot, J. (author), Hamer, R.J. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
This study describes how charge modification affects aggregation of ovalbumin, thereby distinguishing the role of conformational and electrostatic stability in the process. Ovalbumin variants were engineered using chemical methylation or succinylation to obtain a range of protein net charge from -1 to -26. Charge modification significantly...
article 2007
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Broersen, K. (author), Elshof, M. (author), de Groot, J. (author), Voragen, A.G.J. (author), Hamer, R.J. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
A large number of proteins are glycosylated, either in vivo or as a result of industrial processing. Even though the effect of glycosylation on the aggregation of proteins has been studied extensively in the past, some reports show that the aggregation process is accelerated, whereas others found that the process is inhibited by glycosylation....
article 2007
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Wierenga, P.A. (author), Egmond, M.R. (author), Voragen, A.G.J. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
Unfolding of proteins has often been mentioned as an important factor during the adsorption process at air-water interfaces and in the increase of surface pressure at later stages of the adsorption process. This work focuses on the question whether the folding state of the adsorbed protein depends on the rate of adsorption to the interface,...
article 2006
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TNO Kwaliteit van Leven (author), van den Berg, R.A. (author), Hoefsloot, H.C.J. (author), Westerhuis, J.A. (author), Smilde, A.K. (author), van der Werf, M.J. (author)
Background: Extracting relevant biological information from large data sets is a major challenge in functional genomics research. Different aspects of the data hamper their biological interpretation. For instance, 5000-fold differences in concentration for different metabolites are present in a metabolomics data set, while these differences are...
article 2006
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Koppelman, S.J. (author), Nieuwenhuizen, W.F. (author), Gaspari, M. (author), Knippels, L.M.J. (author), Penninks, A.H. (author), Knol, E.F. (author), Hefle, S.L. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
The high resistance of Brazil nut 2S albumin, previously identified as an allergen, against proteolysis by pepsin was examined in this work. Although the denaturation temperature of this protein exceeds the 110 °C at neutral pH, at low pH a fully reversible thermal denaturation was observed at ∼82 °C. The poor digestibility of the protein by...
article 2005
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Walker, L.C. (author), Overstreet, M.A. (author), Siddiqui, A. (author), de Paepe, A. (author), Ceylaner, G. (author), Malfait, F. (author), Symoens, S. (author), Atsawasuwan, P. (author), Yamauchi, M. (author), Ceylaner, S. (author), Bank, R.A. (author), Yeowell, H.N. (author), TNO Preventie en Gezondheid (author)
The clinical diagnosis of a patient with the phenotype of Ehlers-Danlos syndrome type VI was confirmed biochemically by the severely diminished level of lysyl hydroxylase (LH) activity in the patient's skin fibroblasts. A novel homozygous mutation, a single base change of T1360 → G in exon 13 of the LH1 gene, predicted to result in W446G, was...
article 2005
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van Teeffelen, A.M.M. (author), Meinders, M.B.J. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
Information on changes in heat capacity (ΔCp) of proteins upon unfolding is used frequently in literature to understand possible follow-up reactions of protein denaturation, like their aggregation propensity. This thermodynamic property is intrinsic to the protein's architecture and unfolding and should be independent of the approach used to...
article 2005
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van Teeffelen, A.M.M. (author), Broersen, K. (author), de Jongh, H.H.J. (author), TNO Kwaliteit van Leven (author)
Chemical glycosylation of proteins occurs in vivo spontaneously, especially under stress conditions, and has been linked in a number of cases to diseases related to protein denaturation and aggregation. It is the aim of this work to study the origin of the change in thermodynamic properties due to glucosylation of the folded β-lactoglobulin A....
article 2005
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O'Kane, F.E. (author), Happe, R.P. (author), Vereijken, J.M. (author), Gruppen, H. (author), van Boekel, M.A.J.S. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
The gelling characteristics of two vicilin fractions from pea (Pisum sativum L.) were compared over a range of pH and salt conditions after preliminary results showed that despite having equal opportunity to unfold, and expose hydrophobic residues, they had different minimum gelling concentrations (at pH 7.6). Furthermore, at this pH one...
article 2004
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de Jongh, H.H.J. (author), Kosters, H.A. (author), Kudryashova, E. (author), Meinders, M.B.J. (author), Trofimova, D. (author), Wierenga, P.A. (author), TNO Voeding (author)
Using a variety of spectroscopic techniques, a number of molecular functionalities have been studied in relation to the adsorption process of proteins to air-water interfaces. While ellipsometry and drop tensiometry are used to derive information on adsorbed amount and exerted surface pressure, external reflection circular dichroism, infrared,...
article 2004
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Trofimova, D. (author), de Jongh, H.H.J. (author), TNO Voeding (author)
Maillard products of β-lactoglobulin (βLg) and fructose oligosaccharide (FOS) were obtained in different degrees of modification depending on incubation time and pH. By use of a variety of biochemical and spectroscopic tools, it was demonstrated that the modification at limited degrees does not significantly affect the secondary, tertiary, and...
article 2004
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Al-Sheikh, H. (author), Watson, A.J. (author), Lacey, G.A. (author), Punt, P.J. (author), MacKenzie, D.A. (author), Jeenes, D.J. (author), Pakula, T. (author), Penttilä, M. (author), Alcocer, M.J.C. (author), Archer, D.B. (author), TNO Voeding (author)
We describe a new endoplasmic reticulum (ER)-associated stress response in the filamentous fungus Aspergillus niger. The inhibition of protein folding within the ER leads to cellular responses known collectively as the unfolded protein response (UPR) and we show that the selective transcriptional downregulation of the gene encoding glucoamylase,...
article 2004
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Centraal Instituut voor Voedingsonderzoek TNO (author), Pouvreau, L. (author), Gruppen, H. (author), van Koningsveld, G.A. (author), van den Broek, L.A.M. (author), Voragen, A.G.J. (author)
Potato serine protease inhibitor (PSPI) is the most abundant protease inhibitor group in potato tuber. The investigated PSPI isoforms have a highly similar structure at both the secondary and the tertiary level. From the results described, PSPI is classified as a β-II protein based on (1) the presence in the near-UV spectra of sharp peaks,...
article 2004
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Kosters, H.A. (author), Broersen, K. (author), de Groot, J. (author), Simons, J.W.F.A. (author), Wierenga, P. (author), de Jongh, H.H.J. (author), TNO Voeding (author)
Processing of ovalbumin may result in proteins that differ more than 23°C in denaturation temperature while the structural fold is not significantly affected. This is achieved by 1) conversion of positive residues into negative ones (succinylation); 2) elimination of negative charges (methylation); 3) reducing the proteins hydrophobic exposure ...
article 2003
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Koppelman, S.J. (author), Knol, E.F. (author), Vlooswijk, R.A.A. (author), Wensing, M. (author), Knulst, A.C. (author), Hefle, S.L. (author), Gruppen, H. (author), Piersma, S. (author)
Background: Peanut allergen Ara h 3 has been the subject of investigation for the last few years. The reported data strongly depend on recombinant Ara h 3, since a purification protocol for Ara h 3 from peanuts was not available. Methods: Peanut allergen Ara h 3 (glycinin), was purified and its posttranslational processing was investigated. Its...
article 2003
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Kudryashova, E.V. (author), Meinders, M.B.J. (author), Visser, A.J.W.G. (author), van Hoek, A. (author), de Jongh, H.H.J. (author)
The molecular properties of egg white ovalbumin adsorbed at the air/water interface were studied using infrared reflection absorption spectroscopy (IRRAS) and time-resolved fluorescence anisotropy (TRFA) techniques. Ovalbumin adsorbed at the air/ water interface adopts a characteristic partially unfolded conformation in which the content of the...
article 2003
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Stierum, R. (author), Gaspari, M. (author), Dommels, Y. (author), Ouatas, T. (author), Pluk, H. (author), Jespersen, S. (author), Vogels, J. (author), Verhoeckx, K. (author), Groten, J. (author), van Ommen, B. (author)
Here, we describe a proteomics approach to study protein expression changes in differentiating Caco-2 cells. Caco-2 is a colorectal carcinoma cell line, which upon differentiation loses its tumorigenic phenotype and displays characteristics of mature enterocytes, including brush borders with microvilli. Cells were grown in culture flasks and...
article 2003
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Koppelman, S.J. (author), van Koningsveld, G.A. (author), Knulst, A.C. (author), Gruppen, H. (author), Pigmans, I.G.A.J. (author), de Jongh, H.H.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The interaction of the major potato allergen patatin, Sol t 1, with IgE was investigated on a quantitative level as a function of heat treatment at different temperatures. On the basis of a number of publications, potato is considered to be a heat-labile allergen, but the molecular explanation for this behavior was not given. In this work, heat...
article 2002
Searched for: subject%3A%22protein%255C%2Bfolding%22
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