Composition and susceptibility to thrombolysis of human arterial thrombi and the influence of their age

Each of three distinct, concentric layers of human arterial thrombi, was analysed immunochemically for the plasminogen and fibrin content, and for the ex vivo susceptibility to thrombolysis by various thrombolytic agents in a saline or plasma milieu. The age of the thrombus layer determined: (a) the plasminogen content; (b) the fibrin content,...

Regulation of the acute release of tissue-type plasminogen activator from the endothelium by coagulation activation products

Chemicals/CAS: Blood Coagulation Factors; Tissue Plasminogen Activator, EC 3.4.21.68

Characterization of the interaction both in vitro and in vivo of tissue-type plasminogen activator (t-PA) with rat liver cells. Effects of monoclonal antibodies to t-PA

The interaction of 125I-labelled tissue-type plasminogen activator (125I-t-PA) with freshly isolated rat parenchymal and endothelial liver cells was studied. Binding experiments at 4°C with parenchymal cells and endothelial liver cells indicated the presence of 68000 and 44000 high-affinity t-PA-binding sites, with an apparent K(d) of 3.5 and 4...

In vitro diagnosis of fibrinolysis: Present status and desired improvements

Chemicals/CAS: Biological Markers; Plasminogen Activator Inhibitor 1; Tissue Plasminogen Activator, EC 3.4.21.68.

Interactions of halogenated industrial chemicals with transthyretin and effects on thyroid hormone levels in vivo

Previous results in experimental systems have suggested that hydroxylated PCBs may decrease thyroid hormone levels through associative interaction with transthyretin. In the present paper it was investigated whether this property was also shared by various industrial chemicals, mainly pesticides. In total, 65 compounds from 12 chemical groups...

Selenium in diet, blood, and toenails in relation to breast cancer: A case-control study

The association between breast cancer and selenium, measured in the diet, plasma, erythrocytes, and toenails, was investigated in a case-control study in the Netherlands during 1985-1987. Dietary selenium intake was assessed by a structured dietary history technique among 133 breast cancer cases and 238 population controls. Absolute and energy...

Microdetermination of vitamin A in human plasma using high-performance liquid chromatography with fluorescence detection

Chemicals/CAS: n,n dimethylacetamide, 127-19-5; retinol, 68-26-8, 82445-97-4; Vitamin A, 11103-57-4

Comparison of a 52-kDa phosphoprotein from synaptic plasma membranes related to long-term potentiation and the major coated vesicle phosphoprotein

Abstract: In the in vitro hippocampal slice preparation a short tetanus induces long-term potentiation (LTP) and an inerease in the post hoc phosphorylation of a 52-kDa protein in synaptosomal plasma membranes (SPM) prepared from these slices. This 52-kDa SPM phosphoprotein closely resembles the predominant phosphoprotein in coated vesicles,...

Determination of fibrin(ogen) degradation products (FDP) in plasma, using a monoclonal antibody

Determination of glutamine and glutamate in plasma of men and women by ion exchange chromatography

Chemicals/CAS: amino acid, 65072-01-7; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; glutamine, 56-85-9, 6899-04-3; Glutamates; Glutamic Acid, 56-86-0; Glutamine, 56-85-9

Identification of a reversible inhibitor of plasminogen activators in blood plasma

Inhibition of tissue-type plasminogen activator (t-PA) by pooled plasma could be ascribed for only 60% to the endothelial cell type PA inhibitor. The residual inhibition is ascribed to a so-far undescribed plasma component present at 0.2 nmol/l. This component shows reversible binding to t-PA with an apparent K(i) of 10 pmol/l (does not hinder t...

Evidence for the occurrence of a fast-acting inhibitor for tissue-type plasminogen activator in human plasma

Human plasma contains a fast-acting t-PA inhibitor, which is not identical with ??2-antiplasmin or ??2-macroglobulin. The concentration of this inhibitor in normal plasma is highly variable, much lower than that of known plasma protease inhibitors, and in the range of physiologically occurring plasma concentrations of t-PA (0-2 IU/ml). The...

The dependence of the blood level of the oxime HS 6 on the severity of organophosphate poisoning

Atropinised anaesthetised rats were injected i.v. with 4, 6 or 8 x LD50 of the organophosphorous anticholinesterase soman. Subsequent treatment with one dose of HS 6 (100 mg/kg, i.v.) delayed respiratory failure by 1 h or more; a further postponement was obtained when an additional HS 6 infusion was given. At the same infusion rate, HS 6 blood...

The influence of triorthocresylphosphate on the distribution of 32P in the body of the rat after the injection of 32P-sarin

The ChE- and AE- activities and the 32P-concentration of the plasma from rats 1 and 24 hr after s.c. injection of 32P-sarin (200 μg kg) were measured. One hr after the injection the ChE was inhibited to approx. 10 per cent and the AE to 40-50 per cent; 23 hr later 50 per cent of the ChE activity was found to be restored and the AE had recovered...