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Rattink, A.P. (author), Hennis, B.C. (author), Lievers, C.J.A. (author), de Maat, M.P.M. (author), Bertina, R. (author), Mennen, L.I. (author), Rosendaal, F.R. (author), Gaubius instituut TNO (author)Background: In several studies, higher plasma levels of histidine-rich glycoprotein (HRG) have been observed in patients with venous thrombosis than in healthy subjects. Apart from environmental factors, such as the use of oral contraceptives, the plasma HRG levels are mainly determined genetically. The most important genetic determinant is P...article 1999
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Kolkert, W.J. (author), Jamet, F. (author), Prins Maurits Laboratorium TNO (author)Research and technology development is being performed jointly in six subprograms. Next to operational aspects and system studies, railgun, coilgun and electrothermal gun launch is investigated. To power these future gun weapon systems, research on both energy storage, switching, pulse forming networks and hypervelocity projectiles is being...article 1999
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van de Vijver, L.P.L. (author), Weber, C. (author), Kardinaal, A.F.M. (author), Grobbee, D.E. (author), Princen, H.M.G. (author), van Poppel, G. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)Coenzyme Q10 (CoQ10) is an important mitochondrial electron transfer component and has been postulated to function as a powerful antioxidant protecting LDL from oxidative damage. It could thus reduce the risk of cardiovascular disease. Thus far, beneficial effects of supplementation with CoQ10 have been reported. To study the relation between...article 1999
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Braat, E.A.M. (author), Los, P. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)Thrombin cleaves single-chain urokinase-type plasminogen activator (scu- PA) into a virtually inactive two-chain form (tcu-PA/T), a process which may contribute to the maintenance of a fresh blood clot. We have examined the inactivation of scu-PA by thrombin in a plasma milieu to get more insight in the physiological relevance of this phenomenon...article 1998
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- van der Kaaden, M.E. (author), Rijken, D.C. (author), van Berkel, T.J.C. (author), Kuiper, J. (author), Gaubius Instituut TNO (author) article 1998
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Carvalho, N.J.M. (author), Huis in 't Veld, A.J. (author), de Hosson, J.T. (author), TNO Industrie (author)Titanium-nitrogen (TiN) films were Physical Vapour Deposited (PVD) on tool steel substrates with different hardness and surface roughness, in a Bai 640R unit using a triode ion plating (e-gun) with a high plasma density. The coated substrates were submitted to a rolling contact fatigue test technique (modified pin-on-ring test) to obtain some...article 1998
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Slooter, A.J.C. (author), de Knijff, P. (author), Hofman, A. (author), Cruts, M. (author), Breteler, M.M.B. (author), van Broeckhoven, C. (author), Havekes, L.M. (author), van Duijn, C.M. (author), Gaubius Instituut TNO (author)The APOE*4 allele of the apolipoprotein E gene (APOE) is an important risk factor for Alzheimer's disease. It has been suggested that levels of apolipoprotein E (apoE) in plasma are increased in Alzheimer's disease. In this population-based study, we found that serum apoE levels were lower in Alzheimer patients compared to non-demented controls ...article 1998
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Princen, H.M.G. (author), van Duyvenvoorde, W. (author), Buytenhek, R. (author), Blonk, C. (author), Tijburg, L.B.M. (author), Langius, J.A.E. (author), Meinders, A.E. (author), Pijl, H. (author), Gaubius Instituut TNO (author)Intake of flavonoids is associated with a reduced cardiovascular risk. Oxidation of LDL is a major step in atherogenesis, and antioxidants may protect LDL from oxidation. Because tea is an important source of flavonoids which are strong antioxidants, we have assessed in a randomized, placebo-controlled study the effect of consumption of black...article 1998
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Effect of methylglyoxal on the physico-chemical and biological properties of low-density lipoproteinSchalkwijk, C.G. (author), Vermeer, M.A. (author), Stehouwer, C.D.A. (author), te Koppele, J. (author), Princen, H.M.G. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)In patients with diabetes, non-enzymatic glycation of low-density lipoprotein (LDL) has been suggested to be involved in the development of atherosclerosis. α-Dicarbonyl compounds were identified as intermediates in the non-enzymatic glycation and increased levels were reported in patients with diabetes. We studied the effect of the α-dicarbonyl...article 1998
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Centraal Instituut voor Voedingsonderzoek TNO (author), Oostenbrug, G.S. (author), Mensink, R.P. (author), Al, M.D.M. (author), van Houwelingen, A.C. (author), Hornstra, G. (author)During pregnancy, maternal plasma concentrations of the peroxidation-susceptible polyunsaturated fatty acids (polyenes) increase. In addition, the proportion of polyenes is higher in neonatal plasma than in maternal plasma. To study whether these increased amounts of polyenes affect antioxidant levels, we measured lipid-soluble antioxidants in...article 1998
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Noorman, F. (author), Barrett-Bergshoeff, M.M. (author), Bekkers, M. (author), Emeis, J.J. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)Dextran is used during surgery as a prophylactic agent to prevent deep venous thrombosis. Recently it has been shown that dextran increases t-PA plasma concentrations in patients. As dextran is a potential ligand for the mannose receptor, we studied whether this glucose-polymer would be able to inhibit mannose receptor-mediated clearance of t-PA...article 1997
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Brussaard, H.E. (author), Leuven, J.A.G. (author), Kluft, C. (author), Krans, H.M.J. (author), van Duyvenvoorde, W. (author), Buytenhek, R. (author), van der Laarse, A. (author), Princen, H.M.G. (author), TNO Preventie en Gezondheid (author)In type II diabetes mellitus the altered hormonal state after menopause may represent an additional cardiovascular risk factor. Estrogen replacement therapy (ERT) is associated with a decreased cardiovascular risk, at least in nondiabetic post-menopausal women. We studied the effect of ERT on plasma lipids and lipoproteins and on LDL oxidation...article 1997
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Dahl, M.L. (author), Voortman, G. (author), Alm, C. (author), Elwin, C.E. (author), Delbressine, L. (author), Vos, R. (author), Bogaards, J.J.P. (author), Bertilsson, L. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)The novel antidepressant mirtazapine is a racemate with both noradrenergic and serotonergic potentiating effects. In vitro metabolism of racemic mirtazapine was studied in (a) microsomes from cells expressing different cytochrome P450 (CYP) enzymes and (b) human liver microsomes from 10 subjects and compared with the rate of metabolism of 4...article 1997
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Clason, S.B. (author), Meijer, P. (author), Kluft, C. (author), Ersdal, E. (author), Gaubius Instituut TNO (author)A new automated method, COAMATIC® Plasmin Inhibitor, provides a tool for specific determination of Plasmin Inhibitor activity in plasma. The method is adapted to the ACL 300R, Cobas Mira, MLA 1000C and to the Thrombolyzer. Low plasmin concentrations combined with short incubation times ensure good specificity also in the low range, resulting in...article 1996
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Meijer, P. (author), van der Ham, F. (author), Kluft, C. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)The use of Stabilyte® plasma in the Coaliza® t-PA, Coaliza® PAI-1 and Enzygnost® PAP micro was evaluated. For PAI-1 antigen no, for t-PA antigen only small differences were observed between measurements in citrate and Stabilyte® plasma. For PAP-complexes significant lower (72%) results were obtained when Stabilyte® plasma was applied. This is...article 1996
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Kuiper, J. (author), van 't Hof, A. (author), Otter, M. (author), Biessen, E.A.L. (author), Rijken, D.C. (author), van Berkel, T.J.C. (author), Division of Biopharmaceutics, Leiden/Amsterdam Center for Drug Research, Sylvius Laboratory, P. O. Box 9503, 2300 RA Leiden, Netherlands Gaubius Laboratory, TNO Prevention and Health, P.O. Box 430, 2300 AK Leiden, Netherlands (author)The fibrin-specific thrombolyticum tissue-type plasminogen activator (t-PA) has proven to be a potent drug in several clinical trials, but its clinical application is complicated by the rapid clearance of t-PA from the circulation. The rapid plasma clearance of t-PA results from the uptake of t-PA in the liver. t-PA consists of several domains...article 1996
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Gaubius Instituut TNO (author), Sakharov, D.V. (author), Lijnen, H.R. (author), Rijken, D.C. (author)Staphylokinase (STA), a protein of bacterial origin, induces highly fibrin-specific thrombolysis both in human plasma in vitro and in pilot clinical trials. Using fluorescence microscopy, we investigated the spatial distribution of fluorescein isothiocyanate (FITC)-labeled STA during lysis of a plasma clot and its binding to purified fibrin...article 1996
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van Vliet, T. (author), Schreurs, W.H.P. (author), van den Berg, H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Postprandial response curves of β-carotene and retinyl esters in a triglyceride-rich lipoprotein (TRL) fraction were evaluated as a potential measure of β-carotene uptake and cleavage. β-Carotene, retinyl ester, and triglyceride concentrations in the TRI, fraction (density < 1.006 kg/L) and plasma were measured in 10 men for 8 or 16 h after an...article 1995
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Rijken, D.C. (author), Groeneveld, E. (author), Barrett-Bergshoeff, M.M. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)BM 06.022 is a non-glycosylated mutant of human tissue-type plasminogen activator (t-PA) comprising only the kringle-2 and proteinase domains. The in vivo half-life of BM 06.022 antigen is 4- to 5-fold longer than that of t-PA antigen. The in vitro half-life of the activity of BM 06.022 at therapeutic concentrations in plasma is shorter than...article 1994
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Meijer, P. (author), Westerveld, W. (author), Huisman, L.G.M. (author), Gaubius Laboratory TNO Preventie en Gezondheid (author)Comparison of the results for α2-antiplasmin activity of plasma samples (n = 61, range 0-125%) obtained by the 'immediate plasmin inhibition test' (IPIT) and by the automated method for the MLA Electra 1000C of Dade® shows a good correlation (R = 0.935) but a systematic disagreement especially relevant in the range below 40%. An improved method...article 1994