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Smits, M. (author), Nooijen, I. (author), Redegeld, F. (author), de Jong, A. (author), Le, T.M. (author), Knulst, A. (author), Houben, G.F. (author), Verhoeckc, K.C.M. (author)Scope: No accepted and validated methods are currently available which can accurately predict protein allergenicity. In this study, the role of digestion and transport on protein allergenicity is investigated. Methods and results: Peanut allergens (Ara h 1, 2, 3, and 6) and a milk allergen (β-lactoglobulin) are transported across pig intestinal...article 2021
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Patel, N. (author), Adelman, D.C. (author), Anagnostou, K. (author), Baumert, J.L. (author), Blom, W.M. (author), Campbell, D.E. (author), Chinthrajah, R.S. (author), Mills, E.N.C. (author), Javed, B. (author), Purington, N. (author), Remington, B.C. (author), Sampson, H.A. (author), Smith, A.D. (author), Yarham, R.A.R. (author), Turner, P.J. (author)Background Eliciting doses (EDs) (eg, ED01 or ED05 values, which are the amounts of allergen expected to cause objective symptoms in 1% and 5% of the population with an allergy, respectively) are increasingly being used to inform allergen labeling and clinical management. These values are generated from food challenge, but the frequency of...article 2021
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Remington, B.C. (author), Westerhout, J. (author), Meima, M.Y. (author), Blom, W.M. (author), Kruizinga, A.G. (author), Wheeler, M.W. (author), Taylor, S.L. (author), Houben, G.F. (author), Baumert, J.L. (author)Food allergy and allergen management are important global public health issues. In 2011, the first iteration of our allergen threshold database (ATDB) was established based on individual NOAELs and LOAELs from oral food challenge in roughly 1750 allergic individuals. Population minimal eliciting dose (EDp) distributions based on this dataset...article 2020
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de Jongh, H.H.J. (author), de Jong, G.A.H. (author), Apostolovic, D. (author), Taylor, S.L. (author), Baumert, J.L. (author), Koppelman, S.J. (author)This work reports on the effect of heat treatment on the protein conformational stability of intact and post-translationally cleaved peanut allergen Ara h 6 in relation to IgE-binding. Intact and post-translationally cleaved Ara h 6 are structurally similar and their strong resistance to denaturant-induced unfolding is comparable. Only upon...article 2020
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Remington, B.C. (author), Blom, W.M. (author), Bassa, B. (author), Koppelman, S.J. (author)Allergic reactions to meals consumed outside the home are common and can be severe and sometimes fatal. Objective. To quantify the risk reduction potentially achieved by increasing an individual’s threshold sensitivity to peanut (such as by means of immunotherapy) in scenarios of peanut exposure through shared kitchen materials in a restaurant...article 2020
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de Jong, G.A.H. (author), Jayasena, S. (author), Johnson, P. (author), Marsh, J. (author), Apostolovic, D. (author), van Hage, M. (author), Nordlee, J. (author), Baumert, J. (author), Taylor, S.L. (author), Roucairol, C. (author), de Jongh, H. (author), Koppelman, S.J. (author)The 2S albumin Ara h 6 is one of the most important peanut allergens. A post-translationally cleaved Ara h 6 (pAra h 6) was purified from Virginia type peanuts, and the cleavage site was mapped using high-resolution mass spectrometry. Compared to intact Ara h 6, pAra h 6 lacks a 5-amino acid stretch, resembling amino acids 43-47 (UniProt...article 2018
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Remington, B.C. (author), Krone, T. (author), Koppelman, S.J. (author)Background: The clinical relevance of increasing an allergic individual's peanut sensitivity threshold by immunotherapy, that is, eliciting dose (ED) to 300 or 1000 mg peanut protein, has not been previously characterized in a European population. In this study, we quantify the clinical benefits of an increased threshold of reaction following...article 2018
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Koppelman, S.J. (author), Smits, M. (author), Tomassen, M. (author), de Jong, G.A.H. (author), Baumert, J. (author), Taylor, S.L. (author), Witkamp, R. (author), Veldman, R.J. (author), Pieters, R. (author), Wichers, H. (author)The oral mucosa is the first immune tissue that encounters allergens upon ingestion of food. We hypothesized that the bio-accessibility of allergens at this stage may be a key determinant for sensitization. Light roasted peanut flour was suspended at various pH in buffers mimicking saliva. Protein concentrations and allergens profiles were...article 2018
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Smits, M. (author), Le, T.M. (author), Welsing, P. (author), Houben, G. (author), Knulst, A. (author), Verhoeckx, K. (author)Sensitization and allergy to legumes can be influenced by different factors, such as exposure, geographical background, and food processing. Sensitization and the allergic response to legumes differs considerably, however, the reason behind this is not yet fully understood. The aim of this study is to investigate if there is a correlation...article 2018
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Janssen-Duijghuijsen, L.M. (author), Wichers, H.J. (author), van Norren, K. (author), Keijer, J. (author), Baumert, J.L. (author), de Jong, G.A.H. (author), Witkamp, R.F. (author), Koppelman, S.J. (author)Some studies have suggested that allergens may appear in the circulation after ingestion of allergenic food sources. The reported levels of allergen in serum, however, are low, and conclusions between studies differ. Here, we investigated factors that determine the detection of allergens in serum after consumption of peanuts. Ten healthy...article 2017
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Blom, W.M. (author), Kruizinga, A.G. (author), Rubingh, C.M. (author), Remington, B.C. (author), Crevel, R.W.R. (author), Houben, G.F. (author)Refined vegetable oils including refined peanut oil are widely used in foods. Due to shared production processes, refined non-peanut vegetable oils can contain residual peanut proteins. We estimated the predicted number of allergic reactions to residual peanut proteins using probabilistic risk assessment applied to several scenarios involving...article 2017
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Koppelman, S.J. (author), Jayasena, S. (author), Luykx, D. (author), Schepens, E. (author), Apostolovic, D. (author), de Jong, G.A.H. (author), Isleib, T.G. (author), Nordlee, J. (author), Baumert, J. (author), Taylor, S.L. (author), Cheng, H. (author), Maleki, S. (author)Four different market classes of peanut (Runner, Virginia Spanish, and Valencia) are commonly consumed in Western countries, but for some consumers peanuts are a main cause of food-induced anaphylaxis. Limited information is available on the comparative allergenicity of these distinct market classes. The aim of this study was to compare...article 2016
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Remington, B.C. (author), Baumert, J.L. (author), Blom, W.M. (author), Houben, G.F. (author), Taylor, S.L. (author), Kruizinga, A.G. (author)Background Allergens in food may pose a risk to allergic consumers. While there is EU regulation for allergens present as an ingredient, this is not the case for unintended allergen presence (UAP). Food companies use precautionary allergen labels to inform allergic individuals of a potential risk from UAPs. This study investigates the risk of an...article 2015
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Versluis, A. (author), Knulst, A.C. (author), Kruizinga, A.G. (author), Michelsen, A. (author), Houben, G.F. (author), Baumert, J.L. (author), van Os-Medendorp, H. (author)Summary: Food allergic patients have to deal with an avoidance diet. Confusing labelling terms or precautionary labels can result in misinterpretation and risk-taking behaviour. Even those patients that strictly adhere to their diet experience (sometimes severe) unexpected allergic reactions to food. The frequency, severity and causes of such...article 2015
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Verhoeckx, K.C.M. (author), Vissers, Y.M. (author), Baumert, J.L. (author), Faludi, R. (author), Feys, M. (author), Flanagan, S. (author), Herouet-Guicheney, C. (author), Holzhauser, T. (author), Shimojo, R. (author), van der Bolt, N. (author), Wichers, H. (author), Kimber, I. (author)Food processing can have many beneficial effects. However, processing may also alter the allergenic properties of food proteins. A wide variety of processing methods is available and their use depends largely on the food to be processed. In this review the impact of processing (heat and non-heat treatment) on the allergenic potential of proteins...article 2015
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Klemans, R.J.B. (author), Blom, W.M. (author), van Erp, F.C. (author), Masthoff, L.J.N. (author), Rubingh, C.M. (author), van der Ent, C.K. (author), Bruijnzeel-Koomen, C.A.F.M. (author), Houben, G.F. (author), Pasmans, S.G.M.A. (author), Meijer, Y. (author), Knulst, A.C. (author)Background: To improve food labelling strategies, information regarding eliciting doses (EDs) and the effect of patient characteristics on these EDs is necessary. Objective: To establish EDs for objective and subjective symptoms and analyse the effect of sensitization levels and other patient characteristics on threshold distribution curves ...article 2015
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Jayasena, S. (author), Smits, M. (author), Fiechter, D. (author), de Jong, A. (author), Nordlee, J. (author), Baumert, J. (author), Taylor, S.L. (author), Pieters, R.H. (author), Koppelman, S.J. (author)Six commercial peanut enzyme-linked immunosorbent assay kits were assessed for their ability to recover peanut from the standard reference material 2387 peanut butter and also for their specificity in detecting four major peanut allergens, Ara h 1, Ara h 2, Ara h 3, and Ara h 6. The percentage recovery of peanut from peanut butter differed...article 2015
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- Taylor, S.L. (author), Houben, G.F. (author), Baumert, J.L. (author), Crevel, R.R.W.R. (author), Allen, K.J. (author), Dubois, A.E.J. (author), Knulst, A.C. (author), Remington, B.C. (author), Kruizinga, A.G. (author), Blom, W.M. (author), Brooke-Taylor, S. (author) article 2015
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Allen, K.J. (author), Remington, B.C. (author), Baumert, J.L. (author), Crevel, R.W.R. (author), Houben, G.F. (author), Brooke-Taylor, S. (author), Kruizinga, A.G. (author), Taylor, S.L. (author)Background There has been a dramatic proliferation of precautionary labeling by manufacturers to mitigate the perceived risk from low-level contamination from allergens in food. This has resulted in a significant reduction in choice of potentially safe foods for allergic consumers. Objectives We aimed to establish reference doses for 11 commonly...article 2014
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Apostolovic, D. (author), Luykx, D. (author), Warmenhoven, H. (author), Verbart, D. (author), Stanic-Vucinic, D. (author), de Jong, G.A.H. (author), Velickovic, T.C. (author), Koppelman, S.J. (author)Conglutins, the major peanut allergens, Ara h 2 and Ara h 6, are highly structured proteins stabilized by multiple disulfide bridges and are stable towards heat-denaturation and digestion. We sought a way to reduce their potent allergenicity in view of the development of immunotherapy for peanut allergy. Isoforms of conglutin were purified,...article 2013