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Ara h 8, a Bet v 1-homologous allergen from peanut, is a major allergen in patients with combined birch pollen and peanut allergy

Mittag, D. (author), Akkerdaas, J. (author), Ballmer-Weber, B.K. (author), Vogel, L. (author), Wensing, M. (author), Becker, W.M. (author), Koppelman, S.J. (author), Knulst, A.C. (author), Helbling, A. (author), Hefle, S.L. (author), van Ree, R. (author), Vieths, S. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)

We recently described patients with soybean allergy mainly mediated by cross-reactivity to birch pollen allergens. A majority of those patients were reported to have peanut allergy. We sought to study the occurrence of peanut allergy in patients allergic to birch pollen and characterized the Bet v 1-homologous peanut allergen Ara h 8....

article 2004

document

Accumulation of mitochondrial DNA mutations in human immunodeficiency virus-infected patients treated with nucleoside-analogue reverse-transcriptase inhibitors

Martin, A.M. (author), Hammond, E. (author), Nolan, D. (author), Pace, C. (author), Den Boer, M. (author), Taylor, L. (author), Moore, H. (author), Martinez, O.P. (author), Christiansen, F.T. (author), Mallal, S. (author), TNO Preventie en Gezondheid (author)

Nucleoside reverse-transcriptase inhibitor (NRTI) therapy for human immunodeficiency virus (HIV) infection has been associated with mitochondrial DNA (mtDNA) polymerase-γ inhibition and subsequent mtDNA depletion. Effects on mtDNA mutation, although suggested by critical involvement of polymerase-γ in DNA-repair reactions, are unknown. In the...

article 2003

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The role of the Aspergillus niger furin-type protease gene in processing of fungal proproteins and fusion proteins: Evidence for alternative processing of recombinant (fusion-) proteins

Punt, P.J. (author), Drint-Kuijvenhoven, A. (author), Lokman, B.C. (author), Spencer, J.A. (author), Jeenes, D. (author), Archer, D.A. (author), van den Hondel, C.A.M.J.J. (author)

We have characterized growth and protein processing characteristics of Aspergillus niger strains carrying a disrupted allele of the previously cloned and characterized kexB gene [Appl. Environ. Microbiol. 66 (2000) 363] encoding a furin-type endoprotease. Deletion of the single-copy gene confirms it to be non-essential but disruptant strains...

article 2003

document

Genome-wide DNA microarray analysis of Francisella tularensis strains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulent F. tularensis subsp. tularensis

Prins Maurits Laboratorium TNO (author), Broekhuijsen, M.P. (author), Larsson, P. (author), Johansson, A. (author), Byström, M. (author), Eriksson, U. (author), Larsson, E. (author), Prior, R.G. (author), Sjöstedt, A. (author), Titball, R.W. (author), Forsman, M. (author)

Francisella tularensis is a potent pathogen and a possible bioterrorism agent. Little is known, however, to explain the molecular basis for its virulence and the distinct differences in virulence found between the four recognized subspecies, F. tularensis subsp. tularensis, F. tularensis subsp. mediasiatica, F. tularensis subsp. holarctica, and...

article 2003

document

Lactobacilli as live vaccine delivery vectors: Progress and prospects

Seegers, J.F.M.L. (author)

Evidence is accumulating that lactobacilli influence the immune response in a strain-dependent manner. This immunomodulatory capacity is important for the development of the immune response, and also identifies Lactobacillus as a potent oral vaccine carrier. Most of our current knowledge of the use of lactobacilli for vaccination purposes has...

article 2002

document

A tormentor in the quest for plant p53-like proteins

Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Korthout, H.A.A.J (author), Caspers, M.P.M (author), Kottenhagen, M.J. (author), Helmer, Q. (author), Wang, M. (author)

Over the past few years the presence of p53-like proteins in plants was frequently reported, by using the monoclonal antibody Pab240. By means of protein purification and screening a cDNA library, a Pab240 cross-reacting protein and a cDNA clone were isolated from barley. Peptide- and DNA-sequence analysis identified one and the same protein: 2...

article 2002

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Expression of the xylulose 5-phosphate phosphoketolase gene, xpkA, from Lactobacillus pentosus MD363 is induced by sugars that are fermented via the phosphoketolase pathway and is repressed by glucose mediated by CcpA and the mannose phosphoenolpyruvate phosphotransferase system

TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Posthuma, C.C. (author), Bader, R. (author), Engelmann, R. (author), Postma, P.W. (author), Hengstenberg, W. (author), Pouwels, P.H. (author)

Purification of xylulose 5-phosphate phosphoketolase (XpkA), the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene, xpkA, from Lactobacillus pentosus MD363 are described, xpkA encodes a 788-amino-acid protein with a calculated mass of 88,705 Da. Expression of xpkA...

article 2002

document

Isolation and characterization of promoter regions from Streptococcus gordonii CH1

Vriesema, A.J.M. (author), Dankert, J. (author), Zaat, S.A.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

We aimed to identify transcription signal sequences from Streptococcus gordonii strain CH1 by random chromosomal cloning. Five genomic fragments from a Sau3A digest, which constitutively activated transcription of a promoterless spectinomycin resistance gene in this strain, were isolated and characterized. Additionally, one promoter fragment was...

article 1999

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Molecular cloning and functional expression in Lactobacillus plantarum 80 of xylT, encoding the D-xylose-H+ symporter of Lactobacillus brevis

Chaillou, S. (author), Bor, Y.-C. (author), Batt, C.A. (author), Postma, P.W. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding D-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could code for the D-xylulose kinase gene (xylB) and another gene (xylT) encoding a protein of 457 amino acids with significant similarity to the D-xylose-H...

article 1998

document

Expression of heterologous genes in Schizophyllum commune is often hampered by the formation of truncated transcripts

Schuren, F.H.J. (author), Wessels, J.G.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

GPD regulatory sequences were used to express a phleomycin resistance gene (Sh ble) in Schizophyllum commune, resulting in high numbers of phleomycin-resistant transformants. Attempts to express heterologous genes coding for hygromycin B phosphotransferase (hph), aminoglycoside phosphotransferase (apt), β-glucuronidase (uidA) and β-galactosidase...

article 1998

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Cloning, sequence analysis, and characterization of the genes involved in isoprimeverose metabolism in Lactobacillus pentosus

Chaillou, S. (author), Lokman, B.C. (author), Leer, R.J. (author), Posthuma, C. (author), Postma, P.W. (author), Pouwels, P.H. (author)

Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylR binding site (xylO) and a cre-like...

article 1998

document

Expression of cytochromes P450 in mammalian cells.

de Groene, E.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

article 1998

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Isolation and molecular characterisation of the gene encoding eburicol 14α-demethylase (CYP51) from Penicillium italicum

van Nistelrooy, J.G.M. (author), van den Brink, J.M. (author), van Kan, J.A.L. (author), van Gorcom, R.F.M. (author), de Waard, M.A. (author), Prins Maurits Laboratorium TNO (author)

The CYP51 gene encoding eburicol 14α-demethylase (P450(14DM)) was cloned from a genomic library of the filamentous fungal plant pathogen Penicillium italicum, by heterologous hybridisation with the corresponding gene encoding lanosterol 14α-demethylase from the yeast Candida tropicalis. The nucleotide sequence of a 1739-bp genomic fragment and...

article 1996

document

Yeast expressing hepatitis B virus surface antigen determinants on its surface: Implications for a possible oral vaccine

Schreuder, M.P. (author), Deen, C. (author), Boersma, W.J.A. (author), Pouwels, P.H. (author), Klis, F.M. (author), Gaubius Instituut TNO TNO Voeding (author)

The two major hydrophilic regions of the hepatitis B virus surface antigen (HBsAg) have been expressed in the outer mannoprotein layer of the cell wall of 'Bakers Yeast', Saccharomyces cerevisiae, by fusing them between the yeast invertase signal sequence and the yeast α-agglutinin carboxyterminal cell wall anchoring sequence. The fusion protein...

article 1996

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An expression system based on the promoter region of the Aspergillus awamori 1,4-bèta-endoxylanase A gene

Gouka, R.J. (author), Hessing, J.G.M. (author), Punt, P.J. (author), Stam, H. (author), Musters, W. (author), van den Hondel, C.A.M.J.J. (author), TNO Voeding (author)

A new, highly inducible fungal promoter derived from the Aspergillus awamori 1,4-β-endoxylanase A (exlA) gene is described. Induction analysis, carried out with the wild-type strain in shake flasks, showed that exlA expression is regulated at the transcriptional level. Using a β-glucuronidase (uidA) reporter strategy, D-xylose was shown to be an...

article 1996

document

Cloning and characterization of the NADPH cytochrome P450 oxidoreductase gene from the filamentous fungus Aspergillus niger

van den Brink, J.M. (author), van Zeijl, C.M.J. (author), Brons, J.F. (author), van den Hondel, C.A.M.J.J. (author), van Gorcom, R.F.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

In this paper, we describe the cloning and molecular characterization of the Aspergillus niger cytochrome P450 reductase (CPR) gene, cprA. Attempts to clone the cprA gene by heterologous hybridization techniques were unsuccessful. Using the polymerase chain reaction (PCR) with degenerate primers based on conserved regions found in cpr genes from...

article 1995

document

Transient and stable expression of gusA fusions with rice genes in rice, barley and perennial ryegrass

TNO Preventie en Gezondheid Centre for Phytotechnology RUL/TNO (author), Hensgens, L.A.M. (author), de Bakker, E.P.H.M. (author), Os van-Ruygrok, E.P. (author), Rueb, S. (author), van de Mark, F. (author), van der Maas, H.M. (author), van der Veen, S. (author), Kooman-Gersmann, M. (author), Hart, L. (author), Schilperoort, R.A. (author)

Transcriptional and translational fusions were made between the reading frame coding for β-D-glucuronidase and sequences of either a constitutively expressed rice gene (GOS2) involved in initiation of translation or a light-inducible rice gene (GOS5). The transient expression of the fusions was studied via particle bombardment of seedling...

article 1993

document

The promoter of the rice gene GOS2 is active in various different monocot tissues and binds rice nuclear factor ASF-1

de Pater, Sylvia B. (author), van der Mark, F. (author), Rueb, S. (author), Katagiri, F. (author), Chua, N.H. (author), Schilperoort, R.A. (author), Hensgens, L.A.M. (author), Centrum voor Plantenfysiologisch Onderzoek (author)

A single copy gene has been isolated, termed GOS2, from rice. Sequence comparison revealed highly similar genes in mammals and yeast, indicating that GOS2 encodes an evolutionary conserved protein. GOS2 mRNA was detected in all tissues examined. When the upstream region was translationally fused to the reporter gene gusA it was found to drive...

article 1992

document

The construction of new vehicles for the cloning of transcription termination signals

Enger-Valk, B.E. (author), van Rotterdam, J. (author), Pouwels, P.H. (author), Medisch Biologisch Laboratorium TNO (author)

Chemicals/CAS: DNA Restriction Enzymes, EC 3.1.21; DNA, Recombinant; Plasmids; Tryptophan, 73-22-3

article 1981