Searched for: subject%3A%22isotope%255C%2Blabeling%22
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Raaphorst, R.M. (author), Savolainen, H. (author), Cantore, M. (author), van de Steeg, E. (author), van Waarde, A. (author), Colabufo, N.A. (author), Elsinga, P.H. (author), Lammertsma, A.A. (author), Windhorst, A.D. (author), Luurtsema, G. (author)
Positron emission tomography (PET) imaging of P-glycoprotein (P-gp) in the blood-brain barrier can be important in neurological diseases where P-gp is affected, such as Alzheimer’s disease. Radiotracers used in the imaging studies are present at very small, nanomolar, concentration, whereas in vitro assays where these tracers are characterized,...
article 2017
document
van Uhm, J.I.M. (author), Visser, G.W.M. (author), van der Schans, M.J. (author), Geldof, A.A. (author), Meuleman, E.J.H. (author), Nieuwenhuijzen, J.A. (author)
Background: Botulinum neurotoxin A (BoNT-A) is a highly neurotoxic drug and frequently used in patients. Knowledge on the optimal way of administration of BoNT-A and its subsequent distribution is still rather limited. An accurate method for monitoring these processes might be the use of radiolabelled BoNT-A. In this paper, we report our...
article 2015
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Kleinnijenhuis, A.J. (author), Staal, Y.C.M. (author), Duistermaat, E. (author), Engel, R. (author), Woutersen, R.A. (author)
Formaldehyde (FA) is suspected of being associated with the development of leukemia. An inhalation experiment with FA was performed in rats to study whether FA can enter the blood and could thus cause systemic toxicity in remote tissues such as the bone marrow. Therefore, a sophisticated analytical method was developed to detect blood...
article 2013
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Vos, P. (author), Intema, F. (author), van El, B. (author), de Groot, J. (author), Bijlsma, J.W.J. (author), Lafeber, F. (author), Mastbergen, S. (author), TNO Kwaliteit van Leven (author)
Many animal models are used to study osteoarthritis (OA). In these models the role of joint loading in the development of OA is not fully understood. We studied the effect of loading on the development of OA in the canine Groove-model. In ten female beagle dogs OA was induced in one knee according to the Groove-model. The animals were divided in...
article 2009
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de Graaf, A.A. (author), Venema, K. (author), TNO Kwaliteit van Leven (author)
The importance of the human large intestine for nutrition, health, and disease, is becoming increasingly realized. There are numerous indications of a distinct role for the gut in such important issues as immune disorders and obesity-linked diseases. Research on this long-neglected organ, which is colonized by a myriad of bacteria, is a rapidly...
conference paper 2008
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Houben, E. (author), Uchida, Y. (author), Nieuwenhuizen, W.F. (author), de Paepe, K. (author), Vanhaecke, T. (author), Holleran, W.M. (author), Rogiers, V. (author), TNO Kwaliteit van Leven (author)
It has recently become evident that at least five ceramidase (CDase) isoforms are present in human epidermis, and that specifically acidic CDase (aCDase) and alkaline CDase (alkCDase) activities increase during keratinocyte differentiation, and thus might play a pivotal role(s) in permeability barrier function. Prior to investigating their...
article 2007
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Cawello, W. (author), Wolff, H.M. (author), Meuling, W.J.A. (author), Horstmann, R. (author), Braun, M. (author), TNO Kwaliteit van Leven (author)
Background and objective: The dopamine agonist rotigotine has been formulated in a silicone-based transdermal system for once-daily administration. The objective of the present study was to characterise the mass balance of rotigotine in humans after administration of a single transdermal patch containing radiolabelled [14C]rotigotine and to...
article 2007
document
Egert, M. (author), de Graaf, A.A. (author), Maathuis, A. (author), de Waard, P. (author), Plugge, C.M. (author), Smidt, H. (author), Deutz, N.E.P. (author), Dijkema, C. (author), de Vos, W.M. (author), Venema, K. (author), TNO Kwaliteit van Leven (author)
16S rRNA-based stable isotope probing (SIP) and nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling were used to identify bacteria fermenting glucose under conditions simulating the human intestine. The TIM-2 in vitro model of the human intestine was inoculated with a GI tract microbiota resembling that of the small intestine...
article 2007
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Heijboer, A.C. (author), van den Hoek, A.M. (author), Pijl, H. (author), Voshol, P.J. (author), Havekes, L.M. (author), Romijn, J.A. (author), Corssmit, E.P.M. (author), TNO Kwaliteit van Leven (author)
Aims/hypothesis: The present study was conducted to evaluate the effects of central administration of melanotan II (MTII), a melanocortin-3/4 receptor agonist, on hepatic and whole-body insulin sensitivity, independent of food intake and body weight. Methods: Over a period of 24 h, 225 ng of MTII was injected in three aliquots into the left...
article 2005
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TNO Kwaliteit van Leven (author), Goudriaan, J.R. (author), den oer, M.A.M. (author), Rensen, P.C.N. (author), Febbraio, M. (author), Kuipers, F. (author), Romijn, J.A. (author), Havekes, L.M. (author), Voshol, P.J. (author)
CD36 is involved in high-affinity peripheral FFA uptake. CD36-deficient (cd36-/-) mice exhibit increased plasma FFA and triglyceride (TG) levels. The aim of the present study was to elucidate the cause of the increased plasma TG levels in cd36-/- mice. cd36-/- mice showed no differences in hepatic VLDL-TG production or intestinal [3H]TG uptake...
article 2005
document
TNO Voeding (author), van der Woude, H. (author), Alink, G.M. (author), van Rossum, B.E.J. (author), Walle, K. (author), van Steeg, H. (author), Walle, T. (author), Rietjens, I.M.C.M. (author)
This study investigates the role of cellular tyrosinase and/or peroxidase-like oxidative enzyme activity in the covalent binding of quercetin to glutathione, protein, and DNA, as well as the stability of quercetin DNA adducts in time. This was done by studying the formation of glutathionyl quercetin adducts in various in vitro models, and the...
article 2005
document
van Ommen, B. (author), de Bie, A.T.H.J. (author), Bär, A. (author), TNO Voeding (author)
The absorption, disposition, metabolism, and excretion of uniformly 14C-labeled α-cyclodextrin (14C-α-CD) was examined in four separate experiments with Wistar rats. In Experiment 1, 14C-α-CD (25μCi, 50mg/kg bw) was administered intravenously to four male and four female conventional rats. In Experiment 2, 14C-α-CD (25μCi, 200mg/kg bw) was given...
article 2004
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Ronday, H.K. (author), van der Laan, W.H. (author), Tak, P.P. (author), de Roos, J.A.D.M. (author), Bank, R.A. (author), Tekoppele, J.M. (author), Froelich, C.J. (author), Hack, C.E. (author), Hogendoorn, P.C.W. (author), Breedveld, F.C. (author), Verheijen, J.H. (author), Gaubius Instituut (author)
Objective. To investigate the cartilage-degrading capacity of granzyme B and the presence of granzyme B-positive cells at sites of erosion in the rheumatoid synovium. Methods. Granzyme B was added to [3H]proline/[35S]sulphate-labelled cartilage matrices and to cartilage explants. Proteoglycan degradation was assessed by the release of 35S and...
article 2001
document
van de Sandt, J.J.M. (author), Meuling, W.J.A. (author), Elliott, G.R. (author), Cnubben, N.H.P. (author), Hakkert, B.C. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
In vitro and in vivo skin absorption of the pesticide propoxur (2-isopropoxyphenyl N-methyl carbamate, commercially Baygon(TM) and Unden(TM); log Po/w 1.56, MW 209.2) was investigated. In vivo studies were performed in rats and human volunteers, applying the test compound to the dorsal skin and the volar aspect of the forearm, respectively. In...
article 2000
document
van den Heuvel, E.G.H.M. (author), Schoterman, M.H.C. (author), Muijs, T. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The aim of this study was to investigate whether a product rich in transgalactooligosaccharides (TOS, Elix'or) stimulates true Ca absorption in postmenopausal women. The study was a double-blind, randomized crossover study, consisting of two 9-d treatment periods separated by a 19-d washout period. During the treatment periods, 12 subjects drank...
article 2000
document
de Bie, A.T.H.J. (author), van Ommen, B. (author), Bär, A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The absorption, disposition, metabolism, and excretion of 14C-labeled γ-cyclodextrin ([14C]γ-CD) was examined in four separate experiments with Wistar rats. In experiment 1, [14C]γ-CD (25 μCi, 600 mg/kg body wt) was administered intravenously to four male and four female conventional rats. In experiment 2, [14C]γ- CD (25 μCi, 1000 mg/kg body wt)...
article 1998
document
Randerath, K. (author), Sriram, P. (author), Moorthy, B. (author), Aston, J.P. (author), Baan, R.A. (author), van den Berg, P.T.M. (author), Booth, E.D. (author), Watson, W.P. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
32P-postlabelling analysis for detecting DNA adducts formed by polycyclic aromatic compounds is one of the most widely used techniques for assessing genotoxicity associated with these compounds. In cases where the formation of adducts is extremely low, a crucial step in the analysis is an enrichment procedure for adducts prior to the...
article 1998
document
van den Heuvel, E.G.H.M. (author), Schaafsma, G. (author), Muys, T. (author), van Dokkum, W. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The effect of inulin, fructooligosaccharides, and galactooligosaccharides on true intestinal absorption of iron and calcium was measured in men by using double stable-isotope techniques (oral 57Fe and 44Ca and intravenous 58Fe and 48Ca). The incorporation of iron isotopes into erythrocytes and the urinary excretion of calcium isotopes was...
article 1998
document
van den Heuvel, E.G.H.M. (author), Muys, T. (author), Pellegrom, H. (author), Bruyntjes, J.P. (author), van Dokkum, W. (author), Spanhaak, S. (author), Schaafsma, G. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Iron absorption can be measured by the incorporation of stable iron isotopes into erythrocytes, 14 days after isotope administration. The disadvantage of this method is the high dose of isotopes needed to obtain a sufficient enrichment. Therefore, in this study cell fractions rich in young erythroid cells were prepared by using a density...
article 1998
document
Schalkwijk, C.G. (author), Vermeer, M.A. (author), Stehouwer, C.D.A. (author), te Koppele, J. (author), Princen, H.M.G. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
In patients with diabetes, non-enzymatic glycation of low-density lipoprotein (LDL) has been suggested to be involved in the development of atherosclerosis. α-Dicarbonyl compounds were identified as intermediates in the non-enzymatic glycation and increased levels were reported in patients with diabetes. We studied the effect of the α-dicarbonyl...
article 1998
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