Low-density lipoproteins are degraded in HepG2 cells with low efficiency

In previous studies we have shown that in HepG2 cells, as compared with fibroblasts, the low-density lipoprotein (LDL) receptor is only weakly down-regulated upon incubation of the cells with LDL. To explain this difference in down-regulation of the LDL-receptor activity, we studied simultaneously the intracellular processing of 125I-labelled...

Potential of 99mTc-LDLs labeled by two different methods for scintigraphic detection of experimental atherosclerosis in rabbits

In this study we evaluated two different 99mTc-labeling techniques to produce 99mTc-low density lipoprotein (99mTc-LDL) suitable for the scintigraphic delineation of experimental atherosclerotic lesions. The two methods are 1) a procedure that uses stannous chloride and sodium borohydride (borohydride method) and 2) a procedure that uses sodium...

True protein digestibility and amounts of endogenous protein measured with the 15N-dilution technique in piglets fed on peas (Pisum sativum) and common beans (Phaseolus vulgaris)

The faecal and ileal true protein digestibilities of the raw pea (Pisum sativum) varieties finale and frijaune and the ileal true protein digestibility of steam-processed common beans (Phaseolus vulgaris) were measured in piglets using the 15N-dilution technique. The faecal true protein digestibility of both pea varieties was about 97. The ileal...

Partial characterization of low density lipoprotein preparations isolated from fresh and frozen plasma after radiolabeling by seven different methods

Four 99mTc and three 123I labeling methods were evaluated for their suitability to label low density lipoproteins (LDL) for the purpose of scintigraphic biodistribution studies. For 99mTc these methods were: direct incorporation in LDL of 99mTcO4- using sodium dithionite (dithionite method); a method using first N,N-dimethylformamide to prepare...

Reversion of cistron A amber mutants of bacteriophage phiX 174 by ionizing radiation

Amber mutants of bacteriophage can be reverted by ionizing radiation to pseudo wild type particles, i.e. particles able to propagate in a suppressorless host. Since the DNA of phi X 174 is single stranded mutation cannot be due to errors in repair of radiation damage by the excision repair or by the recombination repair system of the host cell,...

Early increase of amino acid transport in stimulated lymphocytes

Chemicals/CAS: 2 amino 2 methylpropionic acid, 62-57-7; carbon, 7440-44-0; thymidine, 50-89-5; tritium, 10028-17-8; Aminoisobutyric Acids; Carbon Isotopes; Lectins; Thymidine, 50-89-5; Tritium, 10028-17-8

The influence of several sterols on the conversion of β-sitosterol into cholesterol in the cockroach

Chemicals/CAS: carbon, 7440-44-0; cholesterol, 57-88-5; Carbon Isotopes; Cholesterol, 57-88-5; Sterols

The binding of sarin in the blood plasma of the rat

Sephadex filtration of serum from rats 10 min after injection of 50 μg/kg of 32P-sarin demonstrated that about 70 per cent of the radioactivity present in the serum was attached to molecules with a molecular weight above 10,000. Incubation of samples of this serum with oximes caused a decrease in the amounts of 32P attached to the large...

The influence of triorthocresylphosphate on the distribution of 32P in the body of the rat after the injection of 32P-sarin

The ChE- and AE- activities and the 32P-concentration of the plasma from rats 1 and 24 hr after s.c. injection of 32P-sarin (200 μg kg) were measured. One hr after the injection the ChE was inhibited to approx. 10 per cent and the AE to 40-50 per cent; 23 hr later 50 per cent of the ChE activity was found to be restored and the AE had recovered...

Radiometric trace analysis of lead