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Beker-van Woudenberg, A.C.R.L. (author), Gröllers-Mulderij, M. (author), Snel, C. (author), Jeurissen, N. (author), Stierum, R. (author), Wolterbeek, A. (author)Reproductive toxicity testing according to the present guidelines requires a high number of animals. Therefore, the development of alternative in vitro methods is urgently required. The aim of the present study was to investigate the applicability domain of the bovine oocyte in vitro maturation assay (bIVM) to study female reproductive...article 2012
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Louisse, J. (author), Verwei, M. (author), Woutersen, R.A. (author), Blaauboer, B.J. (author), Rietjens, I.M.C.M. (author)Introduction: Reliable in vitro and in silico assays as alternatives for in vivo developmental toxicity studies are urgently needed, for the replacement, reduction and refinement (3Rs) of animal use in toxicological research. Therefore, relevant biomarkers for in vivo developmental toxicity in in vitro assays are needed. Areas covered: The...article 2012
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Berg, N. (author), de Wever, B. (author), Fuchs, H.W. (author), Gaca, M. (author), Krul, C.A.M. (author), Roggen, E.L. (author)In November 2009 the In Vitro Testing Industrial Platform (IVTIP) organized a meeting entitled '. Toxicology in the 21st century - working our way towards a visionary reality'. Participating delegates included scientists, key opinion leaders, developers and users of 3Rs-related tests and testing strategies. This paper summarizes the discussions...article 2011
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Punt, A. (author), Schiffelers, M.J.W.A. (author), Jean Horbach, G. (author), van de Sandt, J.J.M. (author), Groothuis, G.M.M. (author), Rietjens, I.M.C.M. (author), Blaauboer, B.J. (author)The present paper aims at identifying strategies to increase the impact and applicability of alternative testing strategies in risk assessment. To this end, a quantitative and qualitative literature evaluation was performed on (a) current research efforts in the development of in vitro methods aiming for alternatives to animal testing, (b) the...article 2011
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Respiratory sensitization: Advances in assessing the risk of respiratory inflammation and irritationVandebriel, R. (author), Callant Cransveld, C. (author), Crommelin, D. (author), Diamant, Z. (author), Glazenburg, B. (author), Joos, G. (author), Kuper, F. (author), Natsch, A. (author), Nijkamp, F. (author), Noteborn, H. (author), Pieters, R. (author), Roberts, D. (author), Roggen, E. (author), Rorije, E. (author), Seed, M. (author), Sewald, K. (author), van den Heuvel, R. (author), van Engelen, J. (author), Verstraelen, S. (author), van Loveren, H. (author)Respiratory sensitization provides a case study for a new approach to chemical safety evaluation, as the prevalence of respiratory sensitization has increased considerably over the last decades, but animal and/or human experimental/predictive models are not currently available. Therefore, the goal of a working group was to design a road map to...article 2011
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- Zhu, Y. (author) article 2011
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Louisse, J. (author), Gönen, S. (author), Rietjens, I.M.C.M. (author), Verwei, M. (author)The present study determines the relative developmental toxicity potencies of retinoids in the embryonic stem (ES)-D3 cell differentiation assay of the embryonic stem cell test, and compares the outcomes with their relative potencies in in vivo and two other in vitro assays for developmental toxicity. The results reveal that the potency ranking...article 2011
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Martinez, R.C.R. (author), Aynaou, A.E. (author), Albrecht, S. (author), Schols, H.A. (author), de Martinis, E.C.P. (author), Zoetendal, E.G. (author), Venema, K. (author), Saad, S.M.I. (author), Smidt, H. (author)Probiotic properties of Lactobacillus amylovorus DSM 16698 were previously demonstrated in piglets. Here, its potential as a human probiotic was studied in vitro, using the TIM-1 system, which is fully validated to simulate the human upper gastrointestinal tract. To evaluate the effect of the food matrix composition on the survival of L....article 2011
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Scott, L. (author), Eskes, C. (author), Hoffmann, S. (author), Adriaens, E. (author), Alepée, N. (author), Bufo, M. (author), Clothier, R. (author), Facchini, D. (author), Faller, C. (author), Guest, R. (author), Harbell, J. (author), Hartung, T. (author), Kamp, H. (author), Varlet, B.L. (author), Meloni, M. (author), McNamee, P. (author), Osborne, R. (author), Pape, W. (author), Pfannenbecker, U. (author), Prinsen, M. (author), Seaman, C. (author), Spielmann, H. (author), Stokes, W. (author), Trouba, K. (author), Berghe, C.V.d. (author), Goethem, F.V. (author), Vassallo, M. (author), Vinardell, P. (author), Zuang, V. (author), TNO Kwaliteit van Leven (author)In spite of over 20 years of effort, no single in vitro assay has been developed and validated as a full regulatory replacement for the Draize Eye Irritation test. However, companies have been using in vitro methods to screen new formulations and in some cases as their primary assessment of eye irritation potential for many years. The present...article 2010
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Buist, H.E. (author), van Burgsteden, J.A. (author), Freidig, A.P. (author), Maas, W.J.M. (author), van de Sandt, J.J.M. (author), TNO Kwaliteit van Leven (author)Most QSARs for dermal absorption predict the permeability coefficient, K<sub>p</sub>, of a molecule, which is valid for infinite dose conditions. In practice, dermal exposure mostly occurs under finite dose conditions. Therefore, a simple model to predict finite dose dermal absorption from infinite dose data (K<sub>p</sub> and lag time) and the...article 2010
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van de Briel, R.J. (author), Pennings, J.L.A. (author), Baken, K.A. (author), Pronk, T.E. (author), Boorsma, A. (author), Gottschalk, R. (author), van Loveren, H. (author), TNO Kwaliteit van Leven (author)Many chemicals can induce allergic contact dermatitis. Because evaluation of skin sensitizing potential by animal testing is prohibited for cosmetics, and screening of many chemicals is required within Registration, Evaluation, Authorisation and Restriction of Chemicals, urgent need exists for predictive in vitro assays to identify contact...article 2010
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de Leeuw van Weenen, J.E. (author), Parlevliet, E.T. (author), Maechler, P. (author), Havekes, L.M. (author), Romijn, J.A. (author), Ouwens, D.M. (author), Pijl, H. (author), Guigas, B. (author), TNO Kwaliteit van Leven (author)Treatment with the dopamine receptor D2 (DRD2) agonist bromocriptine improves metabolic features in obese patients with type 2 diabetes by a still unknown mechanism. In the present study, we investigated the acute effect of bromocriptine and its underlying mechanism(s) on insulin secretion both in vivo and in vitro. For this purpose, C57Bl6/J...article 2010
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Hemery, Y.M. (author), Anson, N.M. (author), Havenaar, R. (author), Haenen, G.R.M.M. (author), Noort, M.W.J. (author), Rouau, X. (author), TNO Kwaliteit van Leven (author)This study evaluated the potential of using ultra-fine grinding and electrostatic separation of wheat bran as methods to improve the bioaccessibility of para-coumaric acid (pCA), sinapic acid (SA) and ferulic acid (FA) from bran-rich breads. Bran fractions were produced and used to bake white bread, whole-grain bread, and seven different bran...article 2010
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Hamer, H.M. (author), Jonkers, D.M.A.E. (author), Renes, I.B. (author), Vanhoutvin, S.A.L.W. (author), Kodde, A. (author), Troost, F.J. (author), Venema, K. (author), Brummer, R.-J.M. (author), TNO Kwaliteit van Leven (author)INTRODUCTION: The colonic mucus layer plays an important role in the protection of the intestinal epithelium and mainly consists of mucin glycoproteins (primarily MUC2 in the colon) trefoil factor 3 (TFF3) and secretory IgA. Butyrate is a major end product of fermentation of dietary fibres and is associated with beneficial effects on colonic...article 2010
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Fernandes, H. (author), Mentink, A. (author), Bank, R. (author), Stoop, R. (author), van Blitterswijk, C. (author), de Boer, J. (author), TNO Kwaliteit van Leven (author)Human multipotent mesenchymal stromal cells (hMSCs) are multipotent cells that, in the presence of appropriate stimuli, can differentiate into different lineages such as the osteogenic, chondrogenic, and adipogenic lineages. In the presence of ascorbic acid, MSCs secrete an extracellular matrix mainly composed of collagen type I. Here we...article 2010
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Eefting, D. (author), Bot, I. (author), de Vries, M.R. (author), Schepers, A. (author), van Bockel, J.H. (author), van Berkel, T.J.C. (author), Biessen, E.A.L. (author), Quax, P.H.A. (author), TNO Kwaliteit van Leven (author)Objective: Inflammatory responses to vascular injury are key events in vein graft disease and accelerated atherosclerosis, which may result in bypass failure. The monocyte chemoattractant protein-1 (MCP-1)/CC-chemokine receptor (CCR)-2 pathway is hypothesized to play a central role. A murine model for vein graft disease was used to study the...article 2009
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Rubbens, M.P. (author), Mol, A. (author), Boerboom, R.A. (author), Bank, R.A. (author), Baaijens, F.P.T. (author), Bouten, C.V.C. (author), TNO Industrie en Techniek TNO Kwaliteit van Leven (author)Tissue-engineered heart valves lack sufficient amounts of functionally organized structures and consequently do not meet in vivo mechanical demands. To optimize tissue architecture and hence improve mechanical properties, various in vitro mechanical conditioning protocols have been proposed, of which intermittent straining is most promising in...article 2009
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End, C. (author), Bikker, F.J. (author), Renner, M. (author), Bergmann, G. (author), Lyer, S. (author), Blaich, S. (author), Hudler, M. (author), Helmke, B. (author), Gassler, N. (author), Autschbach, F. (author), Ligtenberg, A.J.M. (author), Benner, A. (author), Holmskov, U. (author), Schirmacher, P. (author), Nieuw Amerongen, A.V. (author), Rosenstiel, P. (author), Sina, C. (author), Franke, A. (author), Hafner, M. (author), Kioschis, P. (author), Schreiber, S. (author), Poustka, A. (author), Mollenhauer, J. (author)Deleted in malignant brain tumors 1 (DMBT1) is a secreted glycoprotein displaying a broad bacterial-binding spectrum. Recent functional and genetic studies linked DMBT1 to the suppression of LPS-induced TLR4-mediated NF-kappaB activation and to the pathogenesis of Crohn's disease. Here, we aimed at unraveling the molecular basis of its function...article 2009
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Buist, H.E. (author), Schaafsma, G. (author), van de Sandt, J.J.M. (author), TNO Kwaliteit van Leven (author)Quantification of skin absorption is an essential step in reducing the uncertainty of dermal risk assessment. Data from literature indicate that the relative dermal absorption of substances is dependent on dermal loading. Therefore, an internal exposure calculated with absorption data determined at a dermal loading not comparable to the actual...article 2009
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Kienhuis, A.S. (author), van de Poll, M.C.G. (author), Wortelboer, H. (author), van Herwijnen, M. (author), Gottschalk, R. (author), de Jong, C.H.C. (author), Boorsma, A. (author), Paules, R.S. (author), Kleinjans, J.C.S. (author), Stierum, R.H. (author), van Delft, J.H.M. (author), TNO Kwaliteit van Leven (author)The frequent use of rodent hepatic in vitro systems in pharmacological and toxicological investigations challenges extrapolation of in vitro results to the situation in vivo and interspecies extrapolation from rodents to humans. The toxicogenomics approach may aid in evaluating relevance of these model systems for human risk assessment by direct...article 2009