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de Mik, G. (author), de Groot, I. (author), Gerbrandy, J.L.F. (author), Medisch Biologisch Laboratorium TNO Chemisch Laboratorium TNO (author)The effects of ozone and ozonized olefins on aerosol survival of bacteriophage phiX174 were studied. The ozone concentrations used were between 0 and 110 parts/109, giving decay rates up to 0.03 min-1. The olefins used were trans 2 butene and cyclohexene in concentrations of 500 parts/109 and 2.4 parts/106, respectively. Olefins alone have no...article 1977
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Kluft, C. (author), Jenks, R.L. (author), Astrup, T. (author), Gaubius instituut TNO (author)The authors studied the production of fibrinolytic activity in Hageman trait plasma, and conclude that the development of activator activity in euglobulin fractions is delayed, particularly in the Hageman trait plasma, by the presence of C1 inactivator, an inhibitor whose effect can be abolished by the synthetic compound flufenamic acid. Using...article 1977
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Gaubius instituut TNO (author), van Gent, C.M. (author), van der Voort, H.A. (author), de Bruyn, A.M. (author), Klein, F. (author)Two enzymatic methods for the determination of cholesterol in serum have been modified for automatic use. These methods utilize the colour reactions according to Kageyama or Trinder to measure the hydrogen peroxide resulting from the enzymatic reaction. The procedures have been compared with the classical methods according to Abell and to Huang....article 1977
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Veldhuisen, G. (author), Bacchetti, S. (author), van der Valk, M.J. (author), Medisch Biologisch Laboratorium TNO (author)Chemicals/CAS: DNA, Viralarticle 1976
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Davidse, L.C. (author), Werkgroep voor Inwendige Therapie van Plantenziekten TNO (author)Methyl benzimidazol 2 yl carbamate was metabolized by Aspergillus nidulans mycelium to two metabolites, one of which was identified as methyl 5 hydroxybenzimidazol 2 yl carbamate. This compound was further converted to a second metabolite which was not identified. Conversion rate was highest when the culture medium was depleted of nutrients....article 1976
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Lubbe, F.H. (author), Rossi, G. (author), Zaalberg, O.B. (author), Medisch Biologisch Laboratorium TNO (author)A method is described for isolation of antibody forming cells with the help of cluster formation. This was achieved by incubating spleen cells from immunized mice with sheep erythrocytes at 37°C. During incubation rabbit anti mouse immunoglobulin serum was present in order to facilitate the formation of clusters. Thereafter the clusters were...article 1976
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Vos, O. (author), Budke, L. (author), Grant, G.A. (author), Medisch Biologisch Laboratorium TNO (author)In tissue culture, protection against X irradiation by a number of cysteamine derivatives was studied and the results were compared with data obtained in mice. Compounds with a covered SH group, like WR 638, cysteamine phosphate, WR 2721 and AE AE 48527, showed practically no protection when dissolved in tissue culture medium, but developed a...article 1976
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Pouwels, P.H. (author), Pannekoek, H. (author), Medisch Biologisch Laboratorium TNO Chemisch Laboratorium TNO (author)Chemicals/CAS: tryptophan, 6912-86-3, 73-22-3; RNA, Bacterial; RNA, Viral; Tryptophan, 73-22-3; Tryptophan-tRNA Ligase, EC 6.1.1.2article 1976
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- Mattern, I.E. (author), Medisch Biologisch Laboratorium RVO-TNO (author) article 1976
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Nickolson, V.J. (author), Wolthuis, O.L. (author), Medisch Biologisch Laboratorium TNO (author)The effects of Piracetam, Naftidrofuryl and methamphetamine on several parameters of cerebral energy metabolism have been studied. At variance with some reports in the literature neither Piracetam nor Naftidrofuryl affected the cerebral contents of adenine nucleotides and, accordingly, both substances were without effect on the adenylate energy...article 1976
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Nickolson, V.J. (author), Wolthuis, O.L. (author), Medisch Biologisch Laboratorium TNO (author)The effect of Piracetam on rat cerebral protein metabolism in vivo and in vitro was studied. It was found that the drug stimulates the uptake of labelled leucine by cerebral cortex slices, has no effect on the incorporation of leucine into cerebral protein, neither in slices nor in vivo, but inhibits the breakdown of newly formed protein in...article 1976
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Vos, O. (author), Grant, G.A. (author), Budke, L. (author), Medisch Biologisch Laboratorium RVO-TNO (author)The effect of diamide on the radiosensitivity of T cells was investigated under oxic and anoxic conditions. The compound was found to sensitize the cells under both conditions. Under oxic conditions, exposure for 10 min before and during irradiation to 0.1, 0.5 and 1.0 mM diamide produced dose modifying factors of 0.81, 0.60 and 0.55,...article 1976
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Instituut voor experimentele gerontologie TNO (author), Bloemmen, F.J. (author), Radl, J. (author), Haaijman, J.J. (author)Highly purified MOPC 21 IgG1, MOPC 173 IgG(2a), MOPC 195 IgG(2b), MOPC 104 E IgM, and MOPC 315 IgA paraproteins, heterogeneous mouse IgG, Fab and Fc fragments of heterogeneous IgG were prepared and coupled to Sepharose beads. These beads were then used as artificial substrates to test the specificity of fluorescent antisera against mouse...article 1976
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Instituut voor experimentele gerontologie TNO (author), Kruisbeek, A.M. (author)Age related changes in lipopolysaccharide (LPS) and concanavalin A (ConA) responsiveness of rat spleen lymphocytes as judged by 14C TdR incorporation were studied. It was found that responses to both mitogens decreased with advancing age. This report shows that the reduced 14C TdR incorporation could not be attributed to decreased cell survival...article 1976
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Radiobiologisch Instituut TNO (author), Bentvelzen, P. (author), Aarssen, A.M. (author), Brinkhof, J. (author)Primary Rauscher leukemia virus (RLV) induced myeloid leukemias can produce many small clones in agar in the absence of a factor needed for the proliferation of normal myeloid cells. It seems that leukemic cells can more efficiently utilize the small amount of colony stimulating factor (CSF) that it produced by them. At optimal stimulation by...article 1975
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van Hemmen, J.J. (author), Meuling, W.J.A. (author), Bleichrodt, J.F. (author), Medisch Biologisch Laboratorium TNO (author)The radiosensitization by oxygen of biologically active bacteriophage DNA in bacterial extracts was studied. The oxygen effect in such a system appeared not to be due or only to a minor extent to the presence of endogenous sulphydryl compounds. The components in a cell extract which enable oxygen and other sensitizers to sensitize DNA could not...article 1974
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Bleichrodt, J.F. (author), Verheij, W.S.D. (author), Medisch Biologisch Laboratorium TNO (author)Amber mutants of bacteriophage can be reverted by ionizing radiation to pseudo wild type particles, i.e. particles able to propagate in a suppressorless host. Since the DNA of phi X 174 is single stranded mutation cannot be due to errors in repair of radiation damage by the excision repair or by the recombination repair system of the host cell,...article 1973
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Molenaar, P.C. (author), Polak, R.L. (author), Nickolson, V.J. (author), Medisch Biologisch Laboratorium TNO (author)Slices from rat brain cortex were incubated for either 5 or 60 min in a medium containing [3H]choline and 4.7 or 25 mM KCl. Bioassayable ACh and labelled ACh were determined in the incubation medium, in the total tissue homogenate and in subcellular fractions. Raising the KCl concentration from 4.7 to 25 mM stimulated the release and synthesis...article 1973
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Organisch Chemisch Instituut TNO Centraal Instituut voor Voedingsonderzoek TNO (author), Vonk, J.W. (author), Kaars Sijpesteijn, A. (author)Chemicals/CAS: Chlorides; Mercury, 7439-97-6article 1973
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Medisch Biologisch Labortorium TNO (author), Kleijer, W.J. (author), Lohman, P.H.M. (author), Mulder, M.P. (author), Bootsma, D. (author)Repair replication and rejoining of single-strand breaks after X-irradiation in human-skin fibroblasts from normal donors and several patients with xeroderma pigmentosum have been compared. The xeroderma strains showed different levels of repair replication following UV exposure. Repair replication and rejoining of breaks, which are considered...article 1970