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Laman, J.D. (author), Claassen, E. (author), Noelle, R.J. (author), TNO Preventie en Gezondheid (author)Initially, a role for the interaction between CD40, expressed on B cells, and gp39 (CD40L), expressed on activated T cells, has been defined in humoral immunity. CD40-CD40L interaction is an essential signal for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching. CD40-CD40L interaction is...article 1996
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Boot, H.J. (author), Kolen, C.P.A.M. (author), Pouwels, P.H. (author), Prins Maurits Laboratorium TNO (author)The most-dominant surface-exposed protein in many bacterial species is the S-protein. This protein crystallises into a regular monolayer on the outside surface of the bacteria: the S-layer. Lactobacillus acidophilus harbours two S-protein-encoding genes, slpA and slpB, only one of which (slpA) is expressed. In this study, we show by polymerase...article 1996
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Hennis, B.C. (author), Hoffmann, J.J.M.L. (author), Kluft, C. (author), Gaubius Laboratory Haemostasis Division, Department of Clinical Laboratories, Catharina Hospital, Eindhoven, Netherlands TNO Preventie en Gezondheid (author)Recently, two molecular weight forms of Histidine-rich Glycoprotein have been described which explain 59% of the variation in plasma HRG levels. Here, we demonstrate that this can partly be ascribed to a difference in specificity of the immuno assay for HRG towards the two alleles of the polymorphism.article 1996
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Gaubius Instituut TNO (author), van Vlijmen, B.J.M. (author), van Dijk, K.W. (author), van 't Hof, H.B. (author), van Gorp, P.J.J. (author), van der Zee, A. (author), van der Boom, H. (author), Breuer, M.L. (author), Hofker, M.H. (author), Havekesf, L.M. (author)Apolipoprotein E*2(Arg-155 → Cys) (APOE*2) transgenic mice were generated and compared to the previously generated apolipoprotein E*3- Leiden (APOE*3-Leiden) transgenic mice to study the variable expression of hyperlipoproteinemia associated with these two APOE variants. In the presence of the endogenous mouse Apoe gene, the expression of the...article 1996
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Gaubius Instituut TNO (author), Gerritse, K. (author), Laman, J.D. (author), Noelle, R.J. (author), Aruffo, A. (author), Ledbetter, J.A. (author), Boersma, W.J.A. (author), Claassen, E. (author)We investigated the role of CD40-CD40 ligand (CD40L) interactions in multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE). Activated helper T cells expressing CD40L (gp39) surface protein were found in MS patient brain sections, but not in brain tissue sections of normal controls or patients with other neurological diseases....article 1996
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Hennis, B.C. (author), Boomsma, D.I. (author), van Boheemen, P.A. (author), Engesser, L. (author), Kievit, P. (author), Dooijewaard, G. (author), Kluft, C. (author), TNO Preventie en Gezondheid (author)A pedigree-based maximum likelihood method developed by Lange et al. (12) was used to study the contribution of a newly defined di-allelic polymorphism in histidine-rich glycoprotein (HRG) to the plasma levels of HRG. In four families (n = 99) and 20 volunteers we found a heritability of 70%, an age effect of 3% and an effect of individual...article 1995
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Twisk, J. (author), Hoekman, M.F.M. (author), Lehmann, E.M. (author), Meijer, P. (author), Mager, W.H. (author), Princen, H.M.G. (author), TNO Preventie en Gezondheid (author)Evidence from in vivo studies indicates that the bile acid pool and bile acid excretion are increased in humans with diabetes mellitus and in experimental diabetic animals, and that both parameters return to normal levels after administration of insulin. To investigate the biochemical background of these changes, the effects of insulin on bile...article 1995
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Hennis, B.C. (author), van Boheemen, P.A. (author), Wakabayashi, S. (author), Koide, T. (author), Hoffmann, J.J.M.L. (author), Kievit, P. (author), Dooijewaard, G. (author), Jansen, J.G. (author), Klutf, C. (author), TNO Preventie en Gezondheid (author)Two forms of histidine-rich glycoprotein (HRG) were detected on SDS-PAGE by silver staining and immunoblotting after isolation of the protein from pooled plasma using immune-affinity chromatography followed by chromatography with heparin-Sepharose. Both forms were single-chain molecules and the apparent molecular weights of form 1 and form 2...article 1995
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Dobber, R. (author), Tielemans, M. (author), Nagelkerken, L. (author), TNO Preventie en Gezondheid (author)In the present study we investigated whether age-related changes in the composition and functional properties of murine CD4+ T cells are reflected in vivo by a changed humoral response to influenza vaccine in aged mice. After the primary immunization, the titers of influenza-specific IgM, IgG1, IgG2a, and IgG2b, but not of IgG3 and IgE, were...article 1995
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Hennis, B.C. (author), van Boheemen, P.A. (author), Koeleman, B.P.C. (author), Boomsma, D.I. (author), Engesser, L. (author), van Wees, A.G.M. (author), Novakova, I. (author), Brommer, E.J.P. (author), Kluft, C. (author), Gaubius Instituut TNO (author)Recent studies describe families with both elevated plasma HRG levels and thrombosis. In order to study the possibility that allelic variants of the HRG locus are associated with differences in HRG level, we studied linkage between HRG levels and a dinucleotide repeat polymorphism in a Dutch family which was selected on the presence of both...article 1995
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- Kocken, C.H.M. (author), Milek, R.L.B. (author), Lensen, T.H.W. (author), Kaslow, D.C. (author), Schoenmakers, J.G.G. (author), Konings, R.N.H. (author) article 1995
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Foy, T.M. (author), Laman, J.D. (author), Ledbetter, J.A. (author), Aruffo, A. (author), Claassen, E. (author), Noelle, R.J. (author), Medisch Biologisch Laboratorium TNO (author)gp39, the ligand for CD40 expressed on activated CD4+ T helper cells, is required for the generation of antibody responses to T-dependent (TD) antigens. Treatment of mice with anti-gp39 in vivo inhibits both primary and secondary antibody formation to TD, but not T-independent antigens. However, the role of this receptor-ligand pair in the...article 1994
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Heeney, J.L. (author), van Els, C. (author), de Vries, P. (author), ten Haaft, P. (author), Otting, N. (author), Koornstra, W. (author), Boes, J. (author), Dubbes, R. (author), Niphuis, H. (author), Dings, M. (author), Cranage, M. (author), Norley, S. (author), Jonker, M. (author), Bontrop, R.E. (author), Osterhaus, A. (author)To evaluate the effectiveness of vaccine protection from infected cells from another individual of the same species, vaccinated rhesus macaques (Macaca mulatta) were challenged with peripheral blood mononuclear cells from another animal diagnosed with acquired immune deficiency syndrome (AIDS). Half of the simian immunodeficiency virus (SIV)...article 1994
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TNO Preventie en Gezondheid (author), van den Eertwegh, A.J.M. (author), Laman, J.D. (author), Noelle, R.J. (author), Boersma, W.J.A. (author), Claassen, E. (author)T-B cell interactions have a central role in the development of humoral immunity. The binding of a 39 kDa protein (gp39), selectively expressed on activated Th cells, to its receptor CD40, on B cells, results in the initial B cell activation. Thereafter, Th cell derived cytokines regulate the differentiation of B cells into antibody-forming...article 1994
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Koopman, J. (author), Haverkate, F. (author), Grimbergen, J. (author), Lord, S.T. (author), Mosesson, M.W. (author), DiOrio, J.P. (author), Siebenlist, K.S. (author), Legrand, C. (author), Soria, J. (author), Soria, C. (author), Caen, J.P. (author), Instituut voor verouderings- en vaatziekten onderzoek TNO (author)The molecular defect in the abnormal fibrinogen Dusart (Paris V) that is associated with thrombophilia was determined by sequence analysis of genomic DNA that had been amplified using the polymerase chain reaction. The propositus was heterozygous for a single base change (C → T) in the Aα- chain gene, resulting in the amino acid substitution Aα...article 1993
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Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author), HogenEsch, H. (author), de Geus, B. (author), Tielen, F. (author), Rozing, J. (author)We investigated the expression of Ly-6.A2 on isolated murine epidermal cells by flow cytometry. Ly-6.A2 was expressed on 61% of keratinocytes and 6% of dendritic epidermal T cells of C57BL mice. Phosphatidylinositol-specific phospholipase C removed Ly-6.A2, indicating that the antigen is anchored to the keratinocyte membrane via a glycosyl...article 1993
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Medisch Biologisch Laboratorium TNO (author), Laman, J.D. (author), Schellekens, M.M. (author), Abacioglu, Y.H. (author), Lewis, G.K. (author), Tersmette, M. (author), Fouchier, R.A.M. (author), Langedijk, J.P.M. (author), Claassen, E. (author), Boersma, W.J.A. (author)The third variable (V3) domain of the human immunodeficiency virus type 1 (HIV-1) external membrane glycoprotein gp120 is of crucial importance in eliciting neutralizing antibodies in infected persons. Polyclonal (PAb) and monoclonal (MAb) antibodies directed against selected epitopes in the V3 domain are valuable tools for analysis of the...article 1992
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Instituut voor Verouderings- en Vaatziekten Onderzoek TNO Gezondheidsorganisatie TNO (author), Geus, B.de (author), HogenEsch, H. (author), Kubo, R.T. (author), Rozing, J. (author)Murine intestinal intraepithelial lymphocytes (ilEL) represent a heterogeneous population of cells which contains both TCRαβ+ and TCRγδ+ cells. In contrast to the TCRαβ+ cell population in peripheral lymphoid organs, the TCRαβ+ ilEL contain high numbers of Thy-1-cells. This negativity of ilEL for Thy-1, a glycosylphosphatidyl inositol (GPI)...article 1992
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Gaubius instituut TNO (author), van den Berg, E.A. (author), Le Clercq, E. (author), Kluft, C. (author), Koide, T. (author), van der Zee, A. (author), Oldenburg, M. (author), Wijnen TH., J. (author), Meera Khan, P. (author)Histidine-rich glycoprotein (HRG) is a monomeric plasma glycoprotein involved in the modulation of coagulation and fibrinolysis. Using Southern analysis of human-rodent somatic cell hybrid DNA with a human HRG-specific cDNA probe, the HRG gene was assigned to chromosome 3. One hybrid that was known to contain only a segment of chromosome 3 also...article 1990
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Emeis, J.J. (author), van Houwelingen, A.C. (author), van den Hoogen, C.M. (author), Hornstra, G. (author), Gaubius Institute TNO, 2300 AP Leiden, Netherlands (author)For a period of 6 weeks, 76 healthy male volunteers consumed during their daily main meal the contents of one tin (~ 135 g) of either fish (mackerel) paste or meat paste. Fibrinolytic parameters were determined in plasma samples obtained at the beginning and at the end of the experimental period. No changes were found in plasminogen, α2...article 1989