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Bellou, A. (author), Saint-Laudy, J. (author), Knippels, L. (author), Montémont, C. (author), Vauthier, E. (author), Gerard, P. (author), Pellegrom, H. (author), Koerkamp, E.K. (author), Lesesve, J.F. (author), Guéant, J.L. (author), Lambert, H. (author), Mallié, J.P. (author), TNO Voeding (author)Anaphylactic shock is an immunoglobulin E (IgE)-dependent hypersensitivity. Biological tests like leucocyte histamine release (LHR) and human basophil activation (HBA), frequently used in human allergy, reflect both the amount of IgE fixed on cells and the cellular reactivity. To assess whether serum-specific IgE from Brown Norway (BN) rats...article 2003
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Wortelboer, H.M. (author), Usta, M. (author), van der Velde, A.E. (author), Boersma, M.G. (author), Spenkelink, B. (author), van Zanden, J.J. (author), Rietjens, I.M.C.M. (author), van Bladeren, P.J. (author), Cnubben, N.H.P. (author)The multidrug resistance proteins MRP1 and MRP2 are efflux transporters with broad substrate specificity, including glutathione, glucuronide, and sulfate conjugates. In the present study, the interaction of the dietary polyphenol curcumin with MRP1 and MRP2 and the interplay between curcumin-dependent MRP inhibition and its glutathione-dependent...article 2003
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't Hart, B.A. (author), Vogels, J.T.W.E. (author), Spijksma, G. (author), Brok, H.P.M. (author), Polman, C. (author), van der Greef, J. (author), Primatencentrum TNO (author)Proton nuclear magnetic resonance (1H-NMR) spectroscopy in combination with pattern recognition techniques were used to investigate the composition of organic compounds in urines from patients with multiple sclerosis (MS), patients with other neurological diseases (OND) and healthy controls (H). Using a valid animal model of MS, namely the...article 2003
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TNO Preventie en Gezondheid (author), Robinson, W.H. (author), Fontoura, P. (author), Lee, B.J. (author), de Vegvar, H.E.N. (author), Tom, J. (author), Pedotti, R. (author), DiGennaro, C.D. (author), Mitchell, D.J. (author), Fong, D. (author), Ho, P.P.K. (author), Ruiz, P.J. (author), Maverakis, E. (author), Stevens, D.B. (author), Bernard, C.C.A. (author), Martin, R. (author), Kuchroo, V.K. (author), van Noort, J.M. (author), Genain, C.P. (author), Amor, S. (author), Olsson, T. (author), Utz, P.J. (author), Garren, H. (author), Steinman, L. (author)The diversity of autoimmune responses poses a formidable challenge to the development of antigen-specific tolerizing therapy. We developed 'myelin proteome' microarrays to profile the evolution of autoantibody responses in experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis (MS). Increased diversity of autoantibody...article 2003
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Smit, E. (author), Jager, D. (author), Martinez, B. (author), Tielen, F.J. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The structure of the crystallisation domain, SAN, of the S A-protein of Lactobacillus acidophilus ATCC 4356 was analysed by insertion and deletion mutagenesis, and by proteolytic treatment. Mutant S A-protein synthesised in Escherichia coli with 7-13 amino acid insertions near the N terminus or within regions of sequence variation in SAN (amino...article 2002
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Bsibsi, M. (author), Ravid, R. (author), Gveric, D. (author), van Noort, J.M. (author)The family of Toll-like receptors (TLRs) plays a key role in controlling innate immune responses to a wide variety of pathogen-associated molecules. In this study we investigated expression of TLRs in vitro by purified human microglia, astrocytes, and oligodendrocytes, and in vivo by immunohistochemical examination of brain and spinal cord...article 2002
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Versantvoort, C.H.M. (author), Ondrewater, R.C.A. (author), Duizer, E. (author), van de Sandt, J.J.M. (author), Gilde, A.J. (author), Groten, J.P. (author), TNO Voeding (author)Purpose: previous studies have shown that the rat small intestinal cell line IEC-18 provides a size-selective barrier for paracellularly transported hydrophilic macromolecules. In order to determine the utility of IEC-18 cells as an in vitro model to screen the passive paracellular and transcellular components of the intestinal transport of...article 2002
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Gaubius Instituut TNO (author), Gautier, T. (author), Masson, D. (author), Jong, M.C. (author), Duverneuil, L. (author), Guern, N.L. (author), Deckert, V. (author), de Barros, J.P.P. (author), Dumont, L. (author), Bataille, A. (author), Zak, Z. (author), Jiang, X.C. (author), Tall, A.R. (author), Havekes, L.M. (author), Lagrost, L. (author)Transgenic mice expressing human cholesteryl ester transfer protein (HuCETPTg mice) were crossed with apolipoprotein CI-knocked out (apoCI-KO) mice. Although total cholesterol levels tended to be reduced as the result of CETP expression in HuCETPTg heterozygotes compared with C57BL6 control mice (-13%, not significant), a more pronounced...article 2002
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Smit, E. (author), Pouwels, P.H. (author)The N-terminal repeat (SAC1) of the S-protein of Lactobacillus acidophilus bound efficiently and specifically to cell wall fragments (CWFs) when fused to green fluorescent protein, whereas the C-terminal repeat (SAC2) did not. Treatment of CWFs with hydrofluoric acid, but not phenol, prevented binding. Apparently, SAC1 is necessary and...article 2002
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Smit, E. (author), Oling, F. (author), Demel, R. (author), Martinez, B. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Lactobacillus acidophilus, like many other bacteria, harbors a surface layer consisting of a protein (SA-protein) of 43 kDa. SA-protein could be readily extracted and crystallized in vitro into large crystalline patches on lipid monolayers with a net negative charge but not on lipids with a net neutral charge. Reconstruction of the S-layer from...article 2001
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Voshol, P.J. (author), Koopen, N.R. (author), de Vree, J.M.L. (author), Havinga, R. (author), Princen, H.M.G. (author), Oude Elferink, R.P.J (author), Groen, A.K. (author), Kuipers, F. (author), Gaubius Instituut TNO (author)Background/Aims: Mdr2 P-glycoprotein deficiency in mice (Mdr2(-/-)) leads to formation of cholesterol/cholesterol-depleted bile and reduced plasma HDL cholesterol. We addressed the questions: (1) does HDL in Mdr2(-/-) mice normalize upon phospholipid and/or cholesterol feeding, and (2): is the Mdr2(-/-) liver capable of handling excess dietary...article 2001
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Goedde, M.F. (author), Grimbergen, J.M. (author), Toet, K.H. (author), Sitter, T. (author), Quax, P.H. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)Background. The mesothelium has an important role in maintaining an adequate fibrinolytic capacity in the peritoneal cavity and thus in preventing the formation of fibrinous peritoneal adhesions by secreting the fibrinolytic enzyme tissue-type plasminogen activator (t-PA). The fibrinolytic activity of human mesothelial cells (HMCs) is...article 2001
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Koppelman, S.J. (author), Vlooswijk, R.A.A. (author), Knippels, L.M.J. (author), Hessing, M. (author), Knol, E.F. (author), van Reijsen, F.C. (author), Bruijnzeel-Koomen, C.A.F.M. (author)Background: The serology of peanut allergy seems to be different in various parts of the world. We analyzed the composition of 13 samples of three varieties of peanut in order to compare their allergenic nature. Methods: Peanut cultivars that are commonly processed in the West were analyzed for protein content, protein composition, and Ara h 1...article 2001
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Kruisselbrink, A. (author), Heijne Den Bak-Glashouwer, M.J. (author), Havenith, C.E.G. (author), Thole, J.E.R. (author), Janssen, R. (author)Recent evidence suggests that chronic exposure to lactobacilli, which are part of the normal intestinal flora, inhibits the development of allergic disorders. Allergy is mediated by Th2 cells, which produce high levels of IL4 and IL5, and suppressive effects of lactic acid bacteria on the development of allergy have been attributed to their Th1...article 2001
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TNO Preventie en Gezondheid (author), Janssen, R. (author), Kruisselbrink, A. (author), Hoogteijling, L. (author), Lamb, J.R. (author), Young, D.B. (author), Thole, J.E.R. (author)The potential for development of mycobacteria as T helper type 1 (Th1) vaccines capable of induction of Th1 responses to recombinant antigens was explored in a model system based on an immunodominant peptide from house dust mite. Different recombinant mycobacterial preparations were compared for their ability to induce a Th1 response to the...article 2001
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de Boer, P. (author), Duim, B. (author), Rigter, A. (author), van der Plas, J. (author), Jacobs-Reitsma, W.F. (author), Wagenaar, J.A. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)For epidemiological tracing of the thermotolerant Campylobacter species C. jejuni and C. coli, reliable and highly discriminatory typing techniques are necessary. In this study the genotyping techniques of flagellin typing (flaA typing), pulsed-field gel electrophoresis (PFGE), automated ribotyping, and amplified fragment length polymorphism ...article 2000
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Martínez, B. (author), Sillanpää, J. (author), Smit, E. (author), Korhonen, T.K. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting signal of the proteinase, PrtP, of L. casei, CbsA...article 2000
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van Stipdonk, M.J.B. (author), Willems, A.A. (author), Plomp, A.C. (author), van Noort, J.M. (author), Boog, C.J.P. (author), TNO Preventie en Gezondheid (author)The myelin-associated protein, αB-crystallin, is considered a candidate autoantigen in multiple sclerosis (MS). In the present study, we examined the potential of αB-crystallin to induce experimental autoimmune encephalomyelitis (EAE) in Lewis rats. Attempts to induce EAE with either bovine, rat or murine αB-crystallin or αB-crystallin peptides...article 2000
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TNO Preventie en Gezondheid (author), Huisman, J.G. (author), Carotenuto, A. (author), Labrijn, A.F. (author), Papavoine, C.H.M. (author), Laman, J.D. (author), Schellekens, M.M. (author), Koppelman, M.H.G.M. (author), Hilbers, C.W. (author)To identify structural constraints and amino acid sequences important for antibody recognition of the third variable domain (V3) of HIV-1 gp120, we have studied the solution conformation of three 35-mer circular V3 loop peptides derived from HIV-1 strains which differ in syncytium- (SI) and non-syncytium-inducing (NSI) capacity. In addition to...article 2000
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Gijbels, M.J.J. (author), van der Cammen, M. (author), van der Laan, L.J.W. (author), Emeis, J.J. (author), Havekes, L.M. (author), Hofker, M.H. (author), Kraal, G. (author), Gaubius instituut TNO (author)Apolipoprotein E3-Leiden (APOE3-Leiden) transgenic mice develop hyperlipidemia and are highly susceptible to diet-induced atherosclerosis. We have studied the progression and regression of atherosclerosis using immunohistochemistry. Female transgenic mice were fed a moderate fat diet to study atherosclerosis over a longer time period. Fatty...article 1999