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Ballerstedt, H. (author), Volkers, R.J.M. (author), Mars, A.E. (author), Hallsworth, J.E. (author), Santos, V.A.M.D. (author), Puchalka, J. (author), van Duuren, J. (author), Eggink, G. (author), Timmis, K.N. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven (author)Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics and proteomics studies with other P. putida strains, the P. putida KT2440 genomic database serves as standard reference. The utility of KT2440 whole-genome, high-density oligonucleotide microarrays for transcriptomics studies of other Pseudomonas...article 2007
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Lu, Z.F. (author), Zandieh Doulabi, B. (author), Wuisman, P.I. (author), Bank, R.A. (author), Helder, M.N. (author), TNO Kwaliteit van Leven (author)Degenerative disc disease (DDD) is a major cause of chronic low back pain. For mild/intermediate DDD, regeneration by injecting adipose stem cells (ASCs) into the nucleus pulposus (NP) may be considered. The goal of this study is to investigate whether NP cells can direct ASCs towards the NP phenotype. Interactions between NP cells and ASCs were...article 2007
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Bsibsi, M. (author), Bajramovic, J.J. (author), van Duijvenvoorden, E. (author), Persoon, C. (author), Ravid, R. (author), van Noort, J.M. (author), Vogt, M.H.J. (author), TNO Kwaliteit van Leven (author)Human astrocytes express a limited repertoire of Toll-like receptor (TLR) family members including TLR1-4, which are expressed on the cell surface. Also, TLR3 but not TLR4 activation on astrocytes induces expression of several factors involved in neuroprotection and down-regulation of inflammation rather than in the onset of traditional pro...article 2007
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de Mos, M. (author), van El, B. (author), de Groot, J. (author), Jahr, H. (author), van Schie, H.T.M. (author), van Arkel, E.R. (author), Tol, H. (author), Heijboer, R. (author), van Osch, G.J.V.M. (author), Verhaar, J.A.N. (author), TNO Kwaliteit van Leven (author)Background: Understanding biochemical and structural changes of the extracellular matrix in Achilles tendinosis might be important for developing mechanism-based therapies. Hypothesis: In Achilles tendinosis, changes occur in biochemical composition and collagen turnover rate. Study Design: Descriptive laboratory study. Methods: From 10 patients...article 2007
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Kajikawa, A. (author), Satoh, E. (author), Leer, R.J. (author), Yamamoto, S. (author), Igimi, S. (author), TNO Kwaliteit van Leven (author)A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in antigen-specific antibody in...article 2007
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Vis, D.J. (author), Westerhuis, J.A. (author), Smilde, A.K. (author), van der Greef, J. (author), TNO Kwaliteit van Leven (author)Background: Innovative extensions of (M) ANOVA gain common ground for the analysis of designed metabolomics experiments. ASCA is such a multivariate analysis method; it has successfully estimated effects in megavariate metabolomics data from biological experiments. However, rigorous statistical validation of megavariate effects is still...article 2007
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van Vliet, P. (author), Mooijaart, S.P. (author), de Craen, A.J.M. (author), Rensen, P.C.N. (author), van Heemst, D. (author), Westendorp, R.G.J. (author), TNO Kwaliteit van Leven (author)Recently, high plasma apoE levels have been shown to be related to increased cardiovascular mortality, independent of APOE genotype. Here we studied the association of plasma apoE levels with risk of stroke. Within the Leiden 85-plus Study, a prospective population-based study of 561 subjects aged 85 years, we measured plasma apoE level and...bookPart 2007
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Dihal, A.A. (author), Tilburgs, C. (author), van Erk, M.J. (author), Rietjens, I.M.C.M. (author), Woutersen, R.A. (author), Stierum, R.H. (author), TNO Kwaliteit van Leven (author)The aim was to investigate mechanisms contributing to quercetin's previously described effects on cell-proliferation and -differentiation, which contradicted its proposed anticarcinogenic potency. In a 10-day experiment, 40 μM quercetin stabilized by 1 mM ascorbate reduced Caco-2 differentiation up to 50% (p < 0.001). Caco-2 RNA from days 5 and...article 2007
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van Bezooijen, R.L. (author), Svensson, J.P. (author), Eefting, D. (author), Visser, A. (author), van der Horst, G. (author), Karperien, M. (author), Quax, P.H.A. (author), Vrieling, H. (author), Papapoulos, S.E. (author), ten Dijke, P. (author), Löwik, W.G.M.C. (author), TNO Kwaliteit van Leven (author)Sclerostin is an osteocyte-derived negative regulator of bone formation. It inhibits BMP-stimulated bone formation both in vitro and in vivo but has no direct effect on BMP signaling. Instead, sclerostin inhibits Wnt signaling that is required for BMP-stimulated osteoblastic differentiation. Introduction: Sclerostin is a member of the Dan family...article 2007
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Verdoes, J.C. (author), Punt, P.J. (author), Burlingame, R. (author), Bartels, J. (author), van Dijk, R. (author), Slump, E. (author), Meens, M. (author), Joosten, R. (author), Emalfarb, M. (author), TNO Kwaliteit van Leven (author)A self-replicating vector was designed that enables the construction of complex libraries in the fungus Chiysosporium lucknowense. The circular vector is linearized in vivo and results in a transformation frequency up to 13,000 transformants/ug ofplasmid DNA. Upon prolonged cultivation of the transformants in liquid medium, the linearized vector...article 2007
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Kleemann, R. (author), Verschuren, L. (author), van Erk, M.J. (author), Nikolsky, Y. (author), Cnubben, N.H.P. (author), Verheij, E.R. (author), Smilde, A.K. (author), Hendriks, H.F.J. (author), Zadelaar, A.S.M. (author), Smith, G.J. (author), Kaznacheev, V. (author), Nikolskaya, T. (author), Melnikov, A. (author), Hurt-Camejo, E. (author), van der Greef, J. (author), van Ommen, B. (author), Kooistra, T. (author), TNO Kwaliteit van Leven (author)Background: Increased dietary cholesterol intake is associated with atherosclerosis. Atherosclerosis development requires a lipid and an inflammatory component. It is unclear where and how the inflammatory component develops. To assess the role of the liver in the evolution of inflammation, we treated ApoE* 3Leiden mice with cholesterol-free ...article 2007
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Grenier, D. (author), Elliott, E.J. (author), Zurynski, Y. (author), Rodrigues Pereira, R. (author), Preece, M. (author), Lynn, R. (author), von Kries, R. (author), Zimmermann, H. (author), Dickson, N.P. (author), Virella, D. (author), TNO Kwaliteit van Leven (author)Paediatric Surveillance Units (PSUs) have been established in 14 countries and facilitate national, prospective, active surveillance for a range of conditions, with monthly reporting by child health specialists. The International Network of Paediatric Surveillance Units (INoPSU) was established in 1998 and facilitates international collaboration...article 2007
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TNO Kwaliteit van Leven (author), Joost, H.G. (author), Gibney, M.J. (author), Cashman, K.D. (author), Görman, U. (author), Hesketh, J.E. (author), Mueller, M. (author), van Ommen, B. (author), Williams, C.M. (author), Mathers, J.C. (author)Personalised, genotype-based nutrition is a concept that links genotyping with specific nutritional advice in order to improve the prevention of nutrition-associated, chronic diseases. This review describes the current scientific basis of the concept and discusses its problems. There is convincing evidence that variant genes may indeed determine...article 2007
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TNO Kwaliteit van Leven TNO Voeding (author), Leavis, H.L. (author), Willems, R.J.L. (author), van Wamel, W.J.B. (author), Schuren, F.H. (author), Caspers, M.P.M. (author), Bonten, M.J.M. (author)Enterococcus faecium, an ubiquous colonizer of humans and animals, has evolved in the last 15 years from an avirulent commensal to the third most frequently isolated nosocomial pathogen among intensive care unit patients in the United States. E. faecium combines multidrug resistance with the potential of horizontal resistance gene transfer to...article 2007
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TNO Kwaliteit van Leven (author), Pons, D. (author), Monraats, P.S. (author), de Maat, M.P.M. (author), Pires, N.M.M. (author), Quax, P.H.A. (author), van Vlijmen, B.J.M. (author), Rosendaal, F.R. (author), Zwinderman, A.H. (author), Doevendans, P.A.F.M. (author), Waltenberger, J. (author), de Winter, R.J. (author), Tio, R.A. (author), Frants, R.R. (author), van der Laarse, A. (author), van der Wall, E.E. (author), Jukema, J.W. (author)Since activation of the haemostatic system is an important feature of the wound healing response triggered by arterial injury, variations in genes involved in thrombus formation may play a role in restenosis after percutaneous coronary interventions (PCI). Therefore, our aim was to examine the relationship between polymorphisms that are known to...article 2007
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TNO Kwaliteit van Leven (author), Bongaerts, B.W.C. (author), de Goeij, A.F.P.M. (author), de Vogel, S. (author), van den Brandt, P.A. (author), Goldbohm, R.A. (author), Weijenberg, M.P. (author)High alcohol consumption is related to colorectal cancer (CRC). Our objective was to study associations between alcohol consumption and risk of CRC according to characteristics of aetiological pathways: the chromosomal instability (CIN) and the microsatellite instability (MIN) pathway. We classified CIN+ tumours (tumours with either a truncating...article 2007
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TNO Kwaliteit van Leven (author), Alkemade, F.E. (author), de Gittenberger-Groot, A.C. (author), Schiel, A.E. (author), van Munsteren, J.C. (author), Hogers, B. (author), van Vliet, L.S.J. (author), Poelmann, R.E. (author), Havekes, L.M. (author), van Dijk, K.W. (author), de Ruiter, M.C. (author)OBJECTIVE - Maternal hypercholesterolemia is associated with a higher incidence and faster progression of atherosclerotic lesions in neonatal offspring. We aimed to determine whether an in utero environment exposing a fetus to maternal hypercholesterolemia and associated risk factors can prime the murine vessel wall to accelerated development of...article 2007
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TNO Kwaliteit van Leven (author), Moen, C.J.A. (author), Tholens, A.P. (author), Voshol, P.J. (author), de Haan, W. (author), Havekes, L.M. (author), Gargalovic, P. (author), Lusis, A.J. (author), van Dyk, K.W. (author), Frants, R.R. (author), Hofker, M.H. (author), Rensen, P.C.N. (author)The Hyplip2 congenic mouse strain contains part of chromosome 15 from MRL/MpJ on the BALB/cJ background. Hyplip2 mice show increased plasma levels of cholesterol and predominantly triglycerides (TGs) and are susceptible to diet-induced atherosclerosis. This study aimed at elucidation of the mechanism(s) explaining the hypertriglyceridemia....article 2007
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- TNO Kwaliteit van Leven (author), van Ommen, B. (author) article 2007
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Volkers, R.J.M. (author), de Jong, A.L. (author), Hulst, A.G. (author), van Baar, B.L.M. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven TNO Defensie en Veiligheid (author)The aim of this study was to assess the cellular response of the solvent-tolerant Pseudomonas putida S12 to toluene as the single effector. Proteomic analysis (two-dimensional difference-in-gel-electrophoresis) was used to assess the response of P. putida S12 cultured in chemostats. This approach ensures constant growth conditions, both in the...article 2006