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Wang, Y. (author), Xu, H. (author), Wei, X. (author), Chai, C. (author), Xiao, Y. (author), Zhang, Y. (author), Chen, B. (author), Xiao, G. (author), Ouwerkerk, P.B.F. (author), Wang, M. (author), Zhu, Z. (author), TNO Kwaliteit van Leven (author)Monosaccharide transporters mediate the membrane transport of a variable range of monosaccharides, which plays a crucial role in sugar distribution throughout the plant. To investigate the significance of monosaccharide transporters for rice (Oryza sativa L.) seed development, cDNA of a new putative monosaccharide transporter gene OsMST4 was...article 2007
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Weijenberg, M.P. (author), Lüchtenborg, M. (author), de Goeij, A.F.P.M. (author), Brink, M. (author), van Muijen, G.N.P. (author), de Bruïne, A.P. (author), Goldbohm, R.A. (author), van den Brandt, P.A. (author), TNO Kwaliteit van Leven (author)Objective: To investigate baseline fat intake and the risk of colon and rectal tumors lacking MLH1 (mutL homolog 1, colon cancer, nonpolyposis type 2) repair gene expression and harboring mutations in the APC (adenomatous polyposis coli) tumor suppressor gene and in the KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog) oncogene....article 2007
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Emeis, J.J. (author), Jirouskova, M. (author), Muchitsch, E.-M. (author), Shet, A.S. (author), Smyth, S.S. (author), Johnson, G.J. (author), TNO Preventie en Gezondheid (author)Murine blood coagulation factors and function are quite similar to those of humans. Because of this similarity and the adaptability of mice to genetic manipulation, murine coagulation factors and inhibitors have been extensively studied. These studies have provided significant insights into human hemostasis. They have also provided useful...article 2007
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Beulens, J.W.J. (author), Rimm, E.B. (author), Hendriks, H.F.J. (author), Hu, F.B. (author), Manson, J.E. (author), Hunter, D.J. (author), Mukamal, K.J. (author), TNO Kwaliteit van Leven (author)OBJECTIVE - We sought to investigate whether a polymorphism in the alcohol dehydrogenase 1c (ADH1C) gene modifies the association between alcohol consumption and type 2 diabetes. RESEARCH DESIGN AND METHODS - In nested case-control studies of 640 women with incident diabetes and 1,000 control subjects from the Nurses' Health Study and 383 men...article 2007
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Ruijssenaars, H.J. (author), Sperling, E.M.G.M. (author), Wiegerinck, P.H.G. (author), Brands, F.T.L. (author), Wery, J. (author), de Bont, J.A.M. (author), TNO Kwaliteit van Leven (author)A steroid 15β-hydroxylating whole-cell solvent tolerant biocatalyst was constructed by expressing the Bacillus megaterium steroid hydroxylase CYP106A2 in the solvent tolerant Pseudomonas putida S12. Testosterone hydroxylation was improved by a factor 16 by co-expressing Fer, a putative Fe-S protein from Bacillus subtilis. In addition, the...article 2007
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Prado, M. (author), Berben, G. (author), Fumière, O. (author), van Duijn, G. (author), Mensinga-Kruize, J. (author), Reaney, S. (author), Boix, A. (author), von Holst, C. (author), TNO Kwaliteit van Leven (author)The commercialization of animal feeds infected by prions proved to be the main cause of transmission of bovine spongiform encephalopathy (BSE). Therefore, feed bans were enforced, initially for ruminant feeds, and later for all feeds for farmed animals. The development and validation of analytical methods for the species-specific detection of...article 2007
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Goosen, C. (author), Yuan, X.L. (author), van Munster, J.M. (author), Ram, A.F.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)A novel subfamily of putative intracellular invertase enzymes (glycoside hydrolase family 32) has previously been identified in fungal genomes. Here, we report phylogenetic, molecular, and biochemical characteristics of SucB, one of two novel intracellular invertases identified in Aspergillus niger. The sucB gene was expressed in Escherichia...article 2007
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Nijkamp, K. (author), Westerhof, R.G.M. (author), Ballerstedt, H. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven (author)A Pseudomonas putida S12 strain was constructed that is able to convert glucose to p-coumarate via the central metabolite l-tyrosine. Efficient production was hampered by product degradation, limited cellular L-tyrosine availability, and formation of the by-product cinnamate via L-phenylalanine. The production host was optimized by inactivation...article 2007
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Nanni, S. (author), Melandri, G. (author), Hanemaaijer, R. (author), Cervi, V. (author), Tomasi, L. (author), Altimari, A. (author), van Lent, N. (author), Tricoci, P. (author), Bacchi, L. (author), Branzi, A. (author), TNO Kwaliteit van Leven (author)Matrix metalloproteinases (MMPs) are thought to participate in the pathogenesis of coronary artery disease (CAD), particularly in the occurrence of acute coronary syndrome (ACS). Little is known about human in vivo MMP regulation in CAD. The expression and regulation of MMPs and their tissue inhibitors (TIMPs) were evaluated in premature CAD....article 2007
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- van Breda, S.G. (author), Hogervorst, J.G. (author), Schouten, L.J. (author), Knaapen, A.M. (author), van Delft, J.H. (author), Goldbohm, R.A. (author), van Schooten, F.J. (author), van den Brandt, P.A. (author), TNO Kwaliteit van Leven (author) article 2007
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Meulenbelt, I. (author), Kloppenburg, M. (author), Kroon, H.M. (author), Houwing-Duistermaat, J.J. (author), Garnero, P. (author), Hellio-Le Graverand, M.-P. (author), de Groot, J. (author), Slagboom, P.E. (author), TNO Kwaliteit van Leven (author)Objective: To assess the relationship of biochemical markers and radiographic signs of osteoarthritis (ROA) in the subjects with symptomatic osteoarthritis (OA) at multiple sites of the Genetics osteoARthritis and Progression (GARP) study. Methods: We have measured eight biochemical markers, representing tissue turnover of cartilage, bone,...article 2007
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van der Kaaij, R.M. (author), Yuan, X.L. (author), Franken, A. (author), Ram, A.F.J. (author), Punt, P.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)In the genome sequence of Aspergillus niger CBS 513.88, three genes were identified with high similarity to fungal α-amylases. The protein sequences derived from these genes were different in two ways from all described fungal α-amylases: they were predicted to be glycosylphosphatidylinositol anchored, and some highly conserved amino acids of...article 2007
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Smulders, Y.M. (author), Smith, D.E.C. (author), Kok, R.M. (author), Teerlink, T. (author), Gellekink, H. (author), Vaes, W.H.J. (author), Stehouwer, C.D.A. (author), Jakobs, C. (author), TNO Kwaliteit van Leven (author)Background: Red blood cells (RBCs) represent a storage pool for folate. In contrast to plasma, RBC folate can appear in different biochemical isoforms. So far, only the methylenetetrahydrofolate reductase (MTHFR) 677 TT genotype has been identified as a determinant of RBC folate vitamer distribution. Objective: The purpose of this study is to...article 2007
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Monraats, P.S. (author), Kurreeman, F.A.S. (author), Pons, D. (author), Sewgobind, V.D.K.D. (author), de Vries, F.R. (author), Zwinderman, A.H. (author), de Maat, M.P.M. (author), Doevendans, P.A. (author), de Winter, R.J. (author), Tio, R.A. (author), Waltenberger, J. (author), Huizinga, T.W.J. (author), Eefting, D. (author), Quax, P.H.A. (author), Frants, R.R. (author), van der Laarse, A. (author), van der Wall, E.E. (author), Jukema, J.W. (author), TNO Kwaliteit van Leven (author)Genetic factors appear to be important in the process of restenosis after percutaneous coronary intervention (PCI), as well as in inflammation, a pivotal factor in restenosis. An important mediator in the inflammatory response is interleukin (IL)-10. Our aim was to study whether genetic variants in IL-10 predispose to the risk of restenosis. The...article 2007
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Einhorn, R. (author), van Eekhof, J.A.H. (author), Engelberts, A.C. (author), Groeneveld, Y. (author), Verkerk, P.H. (author), Wit, J.M. (author), TNO Kwaliteit van Leven (author)Background: In the Netherlands, preventive child health service (CHS) screening plays an important role in the early detection of congenital, developmental, physical, and mental disorders. Objective: To obtain insight into the referral patterns of children from CHS to general practitioners and from general practitioners to medical specialists....article 2007
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Henneman, P. (author), Schaap, F.G. (author), Havekes, L.M. (author), Rensen, P.C.N. (author), Frants, R.R. (author), van Tol, A. (author), Hattori, H. (author), Smelt, A.H.M. (author), van Dijk, K.W. (author), TNO Kwaliteit van Leven (author)Objective: The recently discovered apoAV is hypothesized to affect triglyceride metabolism by stimulating the lipolysis of triglycerides in VLDL and chylomicrons. We set out to determine the association between increased serum TG levels, plasma apoAV levels, and polymorphism of the APOA5 gene, with specific emphasis on the APOA5 S19W variation....article 2007
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Oude Nijhuis, M.M. (author), van Keulen, J.K. (author), Pasterkamp, G. (author), Quax, P.H. (author), de Kleijn, D.P.V. (author), TNO Kwaliteit van Leven (author)Innate immunity is the first line of defence against invading micro-organisms. The family of Toll-like receptors (TLRs) recognizes pathogen-associated molecular patterns (PAMPs) that are carried by the invading micro-organisms. Infectious pathogens have been implicated to play an important role in atherosclerosis. Nowadays, evidence is...article 2007
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Nueda, M.J. (author), Conesa, A. (author), Westerhuis, J.A. (author), Hoefsloot, H.C.J. (author), Smilde, A.K. (author), Talón, M. (author), Ferrer, A. (author), TNO Kwaliteit van Leven (author)Motivation: Designed microarray experiments are used to investigate the effects that controlled experimental factors have on gene expression and learn about the transcriptional responses associated with external variables. In these datasets, signals of interest coexist with varying sources of unwanted noise in a framework of (co)relation among...article 2007
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Keijser, B.J.F. (author), ter Beek, A. (author), Rauwerda, H. (author), Schuren, F. (author), Montijn, R. (author), van der Spek, H. (author), Brul, S. (author), TNO Kwaliteit van Leven (author)Bacillus subtilis forms dormant spores upon nutrient depletion. Under favorable environmental conditions, the spore breaks its dormancy and resumes growth in a process called spore germination and outgrowth. To elucidate the physiological processes that occur during the transition of the dormant spore to an actively growing vegetative cell, we...article 2007
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Vlaminckx, B.J.M. (author), Schuren, F.H.J. (author), Montijn, R.C. (author), Caspers, M.P.M. (author), Fluit, A.C. (author), Wannet, W.J.B. (author), Schouls, L.M. (author), Verhoef, J. (author), Jansen, W.T.M. (author), TNO Kwaliteit van Leven (author)Group A streptococci (GAS), or Streptococcus pyogenes, are associated with a remarkable variety of diseases, ranging from superficial infections to life-threatening diseases such as toxic-shock-like syndrome (TSS). GAS strains belonging to M types M1 and M3 are associated with TSS. This study aims to obtain insight into the gene profiles...article 2007