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Familiaire hyperlipoproteinemie: Pathofysiologie, genetica, klinische verschijnselen en criteria voor diagnostiek

Havekes, L.M. (author), Frants, R.R. (author), van Stiphout, W.A.H.J. (author), Stalenhoef, A.F.H. (author), Gaubius instituut TNO (author)

Erfelijke vormen van hyperlipoproteïnemie komen voor bij naar schatting 150.000 personen in Nederland. In dit artikel wordt een poging gedaan om een overzicht en diagnostische criteria te geven van een viertal erfelijke hyperlipoproteïnemieën, te weten: 1. familiaire hypercholesterolemie (FH), 2. familiaire dysbètalipoproteïnemie (FD), 3....

article 1989

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In situ hybridization as a tool in cytogenetics

Gezondheidsorganisatie TNO Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author), van Dekken, H. (author)

doctoral thesis 1989

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Regulation of 3-hydroxy-3-methylglutaryl-CoA reductase mRNA contents in human hepatoma cell line Hep G2 by distinct classes of mevalonate-derived metabolites

Gaubius instituut TNO (author), Cohen, L.H. (author), Griffioen, M. (author)

Hep G2 cells were incubated under conditions known to influence the HMG-CoA (3-hydroxy-3-methylglutaryl-CoA) reductase activity, e.g. in the presence of compactin (a competitive inhibitor of HMG-CoA reductase itself) and U18666A (a squalene-2,3-epoxide cyclase inhibitor). We studied the effects of these conditions both on the HMG-CoA reductase...

article 1988

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Human plasminogen activator inhibitor-1 gene. Promoter and structural gene nucleotide sequences

Gaubius Institute TNO, 2313 AD Leiden, Netherlands (author), Bosma, P.J. (author), van den Berg, E.A. (author), Kooistra, T. (author), Siemieniak, D.R. (author), Slightom, J.L. (author)

We have determined the nucleotide sequence of the human plasminogen activator inhibitor-1 (PAI-1) gene and significant stretches of DNA which extend into its 5'- and 3'-flanking DNA regions; a total sequence of 15,867 base pairs (bp) is presented. The sequenced 5'-flanking DNA (1,520 bp) contains the essential eukaryotic cis-type proximal...

article 1988

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Apolipoprotein A-IV polymorhism and its effect on plasma lipid and apolipoprotein concentrations

Gaubius Instituut TNO (author), de Knijff, P. (author), Rosseneu, M. (author), Beisiegel, U. (author), de Keersgieter, W. (author), Frants, R.R. (author), Havekes, L.M. (author)

Recently, we determined the apolipoprotein E (apoE) phenotype distribution in 2,000 randomly selected 35-year-old male individuals by slab gel isoelectric focusing of delipidated plasma samples, followed by immunoblotting using anti-apoE antiserum. These blots have been successfully re-used for immunovisualization of apoA-IV isoelectric focusing...

article 1988

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Nucleotide sequence of the Aspergillus niger trpC gene: Structural relationship with analogous genes of other organisms

Medisch Biologisch Laboratorium TNO (author), Kos, T. (author), Kuijvenhoven, A. (author), Hessing, H.G.M. (author), Pouwels, P.H. (author), van den Hondel, C.A.M.J.J. (author)

Molecular Sequence Numbers: GENBANK: X07071; Chemicals/CAS: tryptophan, 6912-86-3, 73-22-3; DNA Restriction Enzymes, EC 3.1.21; Tryptophan, 73-22-3

article 1988

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Assignment of the human tissue-type plasminogen activator gene (PLAT) to chromosome 8

Verheijen, J.H. (author), Visse, R. (author), Wijnen, T.J. (author), Gaubius Instituut TNO (author)

Using 1.2kb 3'-terminal Pst-I fragment of a full length tissue-type plasminogen activator (t-PA) cDNA clone (ptPA-8FL) and a set of rodent human somatic cell hydrids, the corresponding human gene PLAT was localized on chromosome 8. Chemicals/CAS: plasminogen activator, 9039-53-6; DNA Restriction Enzymes, EC 3.1.21; DNA, 9007-49-2; Genetic...

article 1986

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Changes in the sequence content of albumin mRNA and in its translational activity in the rat liver with age

Horbach, G.J.M.J. (author), Princen, H.M.G. (author), van der Kroef, M. (author), van Bezooijen, C.F.A. (author), Yap, S.H. (author)

To investigate the regulation of age-related changes in albumin synthesis in the rat liver, total postnuclear RNA and polyribosomes, both membrane-bound and free, were prepared from livers of rats of different ages. By the use of a specific complementary DNA probe, the albumin mRNA sequence content was quantitated in these RNA fractions. These...

article 1984

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Effects of amounts and type of agar on the number of spontaneous revertants in the Ames test

Centraal Instituut voor Voedingsonderzoek TNO (author), de Raat, W.K. (author), Willems, M.I. (author), de Meijere, F.A. (author)

It has been shown that the spontaneous revertants in the Ames test can depend on the amount and type of bottom agar on the plates. The most clear-cut effect was found with his G46 strains, carrying the ??uvrB mutation, and with the new hisG428 strain, TA102. Evidence of mutagenic impurities in agar has been found. Chemicals/CAS: agar, 9002-18-0;...

article 1984

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Absence of an effect of lead acetate on sperm morphology, sister chromatid exchanges or on micronuclei formation in rabbits

Willems, M.I. (author), de Schepper, G.G. (author), Wibowo, A.A.E. (author), Immel, H.R. (author), Dietrich, A.J.J. (author), Zielhuis, R.L. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

The influence of lead on sperm morphology, sister chromatid exchanges or on micronuclei formation was studied on male rabbits after exposure to doses of 0, 0.25, and 0.50 mg lead acetate/kg body weight subcutaneously injected three times a week during 14 weeks, each on a group of five rabbits. At the end of exposure phase the lead in blood...

article 1982

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The construction of new vehicles for the cloning of transcription termination signals

Enger-Valk, B.E. (author), van Rotterdam, J. (author), Pouwels, P.H. (author), Medisch Biologisch Laboratorium TNO (author)

Chemicals/CAS: DNA Restriction Enzymes, EC 3.1.21; DNA, Recombinant; Plasmids; Tryptophan, 73-22-3

article 1981

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Micrococcus luteus UV-endonuclease-sensitive sites and sister-chromatid exchanges in Chinese hamster ovary cells

Reynolds, R.J. (author), Natarajan, A.T. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)

Chemicals/CAS: endonuclease, 9055-11-2; Endonucleases, EC 3.1.

article 1979

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Repair of UV-endonuclease-susceptible sites in the 7 complementation groups of xeroderma pigmentosum A through G

Zelle, B. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)

Chemicals/CAS: endonuclease, 9055-11-2; DNA, 9007-49-2; Pyrimidine Dimers

article 1979

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Escherichia coli mutant strain with altered expression of the tryptophan operon: Isolation and preliminary characterization

Pouwels, P.H. (author), Scholten, H.J. (author), Medisch Biologisch Laboratorium TNO (author)

Chemicals/CAS: tryptophan, 6912-86-3, 73-22-3; Anthranilate Synthase, EC 4.1.3.27; Lactose, 63-42-3; RNA, Bacterial; RNA, Messenger; Tryptophan Synthase, EC 4.2.1.20; Tryptophan, 73-22-3

article 1979

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Mutagenicity screening: General principles and minimal criteria. Report of a committee of the European Environmental Mutagen Society

Kilbey, B.J. (author), Igali, S. (author), Lohman, P.H.M. (author), Medisch Biologisch Laboratorium TNO (author)

A statement of general principles and minimal criteria for the screening of chemicals for potential mutagenicity in man that may be used as guidelines for regulatory agencies and industrial organisations. To make clear the potentialities and current limitations of short-term mutagenicity testing for the prediction of genetic and carcinogenic...

article 1978

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Induction of mutations in bacteriophage T7 by γ rays: Independence of host repair mechanisms

Bleichrodt, J.F. (author), Roos, A.L.M. (author), Roos Verheij, W.S.D. (author), Medisch Biologisch Laboratorium TNO (author)

Amber mutants of bacteriophage T7 are reverted by γ-rays to pseudo wild-type particles, i.e. particles able to propagate in a suppressorless host. The yield of revertants is much higher when the phage is irradiated in the presence of oxygen than when irradiated anoxically. Under particular gas conditions the efficiency of mutation induction...

article 1977

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A transcriptional barrier in the regulatory region of the tryptophan operon of Escherichia coli: Its role in the regulation of repressor independent RNA synthesis

Pouwels, P.H. (author), Pannekoek, H. (author), Medisch Biologisch Laboratorium TNO Chemisch Laboratorium TNO (author)

Chemicals/CAS: tryptophan, 6912-86-3, 73-22-3; RNA, Bacterial; RNA, Viral; Tryptophan, 73-22-3; Tryptophan-tRNA Ligase, EC 6.1.1.2

article 1976

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Reversion of cistron A amber mutants of bacteriophage phiX 174 by ionizing radiation

Bleichrodt, J.F. (author), Verheij, W.S.D. (author), Medisch Biologisch Laboratorium TNO (author)

Amber mutants of bacteriophage can be reverted by ionizing radiation to pseudo wild type particles, i.e. particles able to propagate in a suppressorless host. Since the DNA of phi X 174 is single stranded mutation cannot be due to errors in repair of radiation damage by the excision repair or by the recombination repair system of the host cell,...

article 1973

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Evidence for linkage of 3-phosphoglycerate kinase, hypoxanthine-guanine-phosphoribosyl transferase, and glucose 6-phosphate dehydrogenase loci in Chinese hamster cells studied by using a relationship between gene multiplicity and enzyme activity

Medisch Biologisch Labortorium TNO (author), Westerveld, A. (author), Visser, R.P.L.S. (author), Freeke, M.A. (author), Bootsma, D. (author)

The PGK activity was assayed in diploid, hyperdiploid, tetraploid, and hybrid cells all originating from the same Chinese hamster cell line (DON line). A relationship between gene multiplicity and enzyme activity was observed. Selective pressure on the HGPRT locus by growth of hybrid cells in the presence of 8-azaguanine resulted in decreased...

article 1972

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Infection of spheroplasts of Pseudomonas with DNA of bacteriophage PM2

van der Schans, G.P. (author), Weyermans, J.P. (author), Bleichrodt, J.F. (author), Medisch Biologisch Laboratorium TNO (author)

Spheroplasts of Pseudomonas BAL-31/PM2, obtained by treatment of the bacteria with lysozyme, can be infected with purified DNA from bacteriophage PM2. After 4 h of incubation the yield of progeny phage reaches a value of 107-6×107 plaque forming units/μg PM2 DNA. The yield increases linearly with the concentration of DNA over at least 3 orders...

article 1971