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Zancanella, V. (author), Vallès, A. (author), Liefhebber, J.M.P. (author), Paerels, L. (author), Vendrell Tornero, C. (author), Wattimury, H. (author), van der Zon, T. (author), van Rooijen, K. (author), Golinska, M. (author), Grevelink, T. (author), Ehlert, E. (author), Pieterman, E.J. (author), Keijzer, N. (author), Princen, H.M.G. (author), Stokman, G. (author), Liu, Y.P. (author)A gene-silencing platform (miQURE) has been developed and successfully used to deliver therapeutic microRNA (miRNA) to the brain, reducing levels of neurodegenerative disease-causing proteins/RNAs via RNA interference and improving the disease phenotype in animal models. This study evaluates the use of miQURE technology to deliver therapeutic...article 2023
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Inia, J.A. (author), Stokman, G. (author), Pieterman, E.J. (author), Morrison, M.C. (author), Menke, A.L. (author), Verschuren, L. (author), Caspers, M.P.M. (author), Giera, M. (author), Jukema, J.W. (author), van den Hoek, A.M. (author), Princen, H.M.G. (author)Patients with metabolic syndrome are often prescribed statins to prevent the development of cardiovascular disease. Conversely, data on their effects on non-alcoholic steatohepatitis (NASH) are lacking. We evaluated these effects by feeding APOE*3-Leiden mice a Western-type diet (WTD) with or without atorvastatin to induce NASH and hepatic...article 2023
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Inia, J.A. (author), Stokman, G. (author), Morrisoon, M.C. (author), Worms, N. (author), Verschuren, L. (author), Caspers, M.P.M. (author), Menke, A.L. (author), Petitjean, L. (author), Chen, L. (author), Petitjean, M. (author), Julema, J.W. (author), Princen, H.M.G. (author), van den Hoek, A.M. (author)Semaglutide, a glucagon-like peptide-1 receptor agonist, is an antidiabetic medication that has recently been approved for the treatment of obesity as well. Semaglutide is postulated to be a promising candidate for the treatment of non-alcoholic steatohepatitis (NASH). Here, Ldlr-/-.Leiden mice received a fast-food diet (FFD) for 25 weeks,...article 2023
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Suchowerska, A.K. (author), Stokman, G. (author), Palmer, J.T. (author), Coghlan, P.A. (author), Pieterman, E.J. (author), Keijzer, N. (author), Lambert, G. (author), Chemello, K. (author), Jaafar, A.K. (author), Parmar, J. (author), Yan, L. (author), Tong, Y. (author), Mu, L. (author), Princen, H.M.G. (author), Bonnar, J. (author), Evison, B.J. (author)Proprotein convertase subtilisin kexin type 9 (PCSK9) inhibits the clearance of low-density lipoprotein (LDL) cholesterol (LDL-C) from plasma by directly binding with the LDL receptor (LDLR) and sending the receptor for lysosomal degradation. As the interaction promotes elevated plasma LDL-C levels, and therefore a predisposition to...article 2022
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Grinberg, M. (author), Stöber, R.M. (author), Edlund, K. (author), Rempel, E. (author), Godoy, P. (author), Reif, R. (author), Widera, A. (author), Madjar, K. (author), Schmidt-Heck, W. (author), Marchan, R. (author), Sachinidis, A. (author), Spitkovsky, D. (author), Hescheler, J. (author), Carmo, H. (author), Arbo, M.D. (author), van de Water, B. (author), Wink, S. (author), Vinken, M. (author), Rogiers, V. (author), Escher, S. (author), Hardy, B. (author), Mitic, D. (author), Myatt, G. (author), Waldmann, T. (author), Mardinoglu, A. (author), Damm, G. (author), Seehofer, D. (author), Nüssler, A. (author), Weiss, T.S. (author), Oberemm, A. (author), Lampen, A. (author), Schaap, M.M. (author), Luijten, M. (author), van Steeg, H. (author), Thasler, W.E. (author), Kleinjans, J.C.S. (author), Stierum, R.H. (author), Leist, M. (author), Rahnenführer, J. (author), Hengstler, J.G. (author)Correction to: Arch Toxicol (2014) 88:2261–2287 https://doi.org/10.1007/s00204-014-1400-x.article 2022
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Krishnan, S. (author), Vermeulen, R. (author), Pronk, A. (author), Gyori, B.M. (author), Bachman, J.A. (author), Vlaanderen, J. (author), Stierum, R. (author)BACKGROUND: Mechanistic data is increasingly used in hazard identification of chemicals. However, the volume of data is large, challenging the efficient identification and clustering of relevant data. OBJECTIVES: We investigated whether evidence identification for hazard assessment can become more efficient and informed through an automated...article 2022
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Syvertsson, S. (author), Wang, B. (author), Staal, J. (author), Gao, Y. (author), Kort, R. (author), Hamoen, L.W. (author)To cope with sudden changes in their environment, bacteria can use a bet-hedging strategy by dividing the population into cells with different properties. This so-called bimodal or bistable cellular differentiation is generally controlled by positive feedback regulation of transcriptional activators. Due to the continuous increase in cell volume...article 2021
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van Bilsen, J.H.M. (author), Dulos, R. (author), van Stee, M.F. (author), Meima, M.Y. (author), Rouhani Rankouhi, T. (author), Neergaard Jacobsen, L. (author), Staudt Kvistgaard, A. (author), Garthoff, J.A. (author), Knippels, L.M.J. (author), Knipping, K. (author), Houben, G.F. (author), Verschuren, L. (author), Meijerink, M. (author), Krishnan, S. (author)A healthy immune status is strongly conditioned during early life stages. Insights into the molecular drivers of early life immune development and function are prerequisite to identify strategies to enhance immune health. Even though several starting points for targeted immune modulation have been identified and are being developed into...article 2020
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Oosterman, J.E. (author), Wopereis, S. (author), Kalsbeek, A. (author)Obesity and type 2 diabetes (T2D) have become a global health concern. The prevalence of obesity and T2D is significantly higher in shift workers compared to people working regular hours. An accepted hypothesis is that the increased risk for metabolic health problems arises from aberrantly timed eating behavior, that is, eating out of synchrony...article 2020
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Tans, R. (author), van Diepen, J.A. (author), Bijlsma, S. (author), Verschuren, L. (author), Suppers, A. (author), Stienstra, R. (author), Wevers, R.A. (author), Tack, C.J. (author), Gloerich, J. (author), van Gool, A.J. (author)Background: Overweight and obesity can lead to adipose tissue inflammation, which causes insulin resistance and on the long-term type 2 diabetes mellitus (T2D). The inflammatory changes of obese-adipose tissue are characterized by macrophage infiltration and activation, but validated circulating biomarkers for adipose tissue inflammation for...article 2019
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Remels, A.H.V. (author), Derks, W.J.A. (author), Cillero-Pastor, B. (author), Verhees, K.J.P. (author), Kelders, M.C. (author), Heggermont, W. (author), Carai, P. (author), Summer, G. (author), Ellis, S.R. (author), de Theije, C.C. (author), Heeren, R.M.A. (author), Heymans, S. (author), Papageorgiou, A.P. (author), van Bilsen, M. (author)Acute viral myocarditis (VM), characterised by leukocyte infiltration and dysfunction of the heart, is an important cause of sudden cardiac death in young adults. Unfortunately, to date, the pathological mechanisms underlying cardiac failure in VM remain incompletely understood. In the current study, we investigated if acute VM leads to cardiac...article 2018
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Morrison, M.C. (author), Kleemann, R. (author), van Koppen, A. (author), Hanemaaijer, R. (author), Verschuren, L. (author)Introduction: It is generally accepted that metabolic inflammation in the liver is an important driver of disease progression in NASH and associated matrix remodeling/fibrosis. However, the exact molecular inflammatory mechanisms are poorly defined in human studies. Investigation of key pathogenic mechanisms requires the use of pre-clinical...article 2018
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Fiamoncini, J. (author), Yiorkas, A.M. (author), Gedrich, K. (author), Rundle, M. (author), Alsters, S.I. (author), Roeselers, G. (author), van den Broek, T.J. (author), Clavel, T. (author), Lagkouvardos, I. (author), Wopereis, S. (author), Frost, G. (author), van Ommen, B. (author), Blakemore, A.I. (author), Daniel, H. (author)Bile acids (BA) are signaling molecules with a wide range of biological effects, also identified among the most responsive plasma metabolites in the postprandial state. We here describe this response to different dietary challenges and report on key determinants linked to its interindividual variability. Healthy men and women (n = 72, 62 ± 8 yr,...article 2017
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Hoeke, G. (author), Wang, Y. (author), van Dam, A.D. (author), Mol, M. (author), Gart, E. (author), Klop, H.G. (author), van den Berg, S.M. (author), Pieterman, E.H. (author), Princen, H.M.G. (author), Groen, A.K. (author), Rensen, P.C.N. (author), Berbée, J.F.P. (author), Boon, M.R. (author)Background and aims Activation of brown adipose tissue (BAT) reduces both hyperlipidemia and atherosclerosis by increasing the uptake of triglyceride-derived fatty acids by BAT, accompanied by formation and clearance of lipoprotein remnants. We tested the hypothesis that the hepatic uptake of lipoprotein remnants generated by BAT activation...article 2017
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Kooter, I.M. (author), Grollers-Mulderij, M. (author), Duistermaat, E. (author), Kuper, C.F. (author)Mucilair 3D bronchial airway models, cultured at an air-liquid interface, were exposed to aerosols of copper oxide (CuO) nanoparticles in Vitrocell air exposure modules. Four cell donors, four exposure modules and four exposure concentrations were varied within four different exposure sessions using a statistical experimental design called a...article 2017
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Mariman, R. (author), Reefman, E. (author), Tielen, F. (author), Persoon-Deen, C. (author), van de Mark, K. (author), Worms, N. (author), Koning, F. (author), Nagelkerken, L. (author)Genetic predisposition and environmental factors, including the gut microbiota, have been suggested as major factors in the development and progression of atopic dermatitis. Hyperlipidemic human APOC1+/+ transgenic mice display many features of human atopic dermatitis, such as scaling, lichenification, excoriations, and pruritus, along with a...article 2016
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Higuera, G.A. (author), Fernandes, H. (author), Spitters, T.W.G.M. (author), van de Peppel, J. (author), Aufferman, N. (author), Truckenmueller, R. (author), Stoop, R. (author), van Leeuwen, J.P. (author), de Boer, J. (author), Subramaniam, V. (author), Karperien, M. (author), van Blitterswijk, C. (author), van Boxtel, A. (author), Moroni, L. (author)Cells and tissues are intrinsically adapted to molecular gradients and use them to maintain or change their activity. The effect of such gradients is particularly important for cell populations that have an intrinsic capacity to differentiate into multiple cell lineages, such as bone marrow derived mesenchymal stromal cells (MSCs). Our results...article 2015
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Liang, W. (author), Verschuren, L. (author), Mulder, P. (author), van der Hoorn, J.W.A. (author), Verheij, J. (author), van Dam, A.D. (author), Boon, M.R. (author), Princen, H.M.G. (author), Havekes, L.M. (author), Kleemann, R. (author), van den Hoek, A.M. (author)BACKGROUND AND PURPOSE: Salsalate (salicylsalicylic acid) is an anti-inflammatory drug that was recently found to exert beneficial metabolic effects on glucose and lipid metabolism. Although its utility in the prevention and management of a wide range of vascular disorders, including type 2 diabetes and metabolic syndrome has been suggested...article 2015
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Mariman, R. (author), Tielen, F. (author), Koning, F. (author), Nagelkerken, L. (author)Background: Probiotic bacteria may render mice resistant to the development of various inflammatory and infectious diseases. Objective: This study aimed to identify mechanisms by which probiotic bacteria may influence intestinal immune homeostasis in noninflammatory conditions. Methods: The effect of VSL#3, a mixture of 8 probiotic bacteria, on...article 2015
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Goelela, V.S. (author), Kelder, T. (author), Adriaens, M.E. (author), Evelo, C.T. (author), Radonijc, M. (author)Background Illumina whole-genome expression bead arrays are a widely used platform for transcriptomics. Most of the tools available for the analysis of the resulting data are not easily applicable by less experienced users. ArrayAnalysis.org provides researchers with an easy-to-use and comprehensive interface to the functionality of R and...article 2015