Searched for: subject%3A%22fibrinolysis%22
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de Fouw, N.J. (author), Haverkate, F. (author), Bertina, R.M. (author), Gaubius Instituut TNO (author)
The effect of purified human activated protein C (APC) and protein S on fibrinolysis was studied by using an in vitro blood clot lysis technique. Blood clots were formed from citrated blood (supplemented with 125I-fibrinogen) by adding thrombin and Ca2+-ions; lysis of the clots was achieved by adding tissue-type plasminogen activator. The...
article 1986
document
Gaubius instituut TNO (author), Boks, A.L. (author), Brommer, E.J.P. (author), Schalm, S.W. (author), van Vliet, H.H.D.M. (author)
In a study of severe, decompensated liver failure, we tried to find a correlation between hemorrhage and parameters of hemostasis and fibrinolysis. Three groups of patients were studied: alcoholic cirrhosis; nonalcoholic cirrhosis, and acute liver failure without known prior liver disease. The two cirrhotic groups did not differ significantly...
article 1986
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Koppert, P.W. (author), Koopman, J. (author), Haverkate, F. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)
Balb/c mice were immunized with a mixture of fibrin degradation products (XDPs) prepared by complete lysis of a human bloot clot by tissue-type plasminogen activator and purified by immunoaffinity chromatography. Spleen cells of the mice were fused with P3 x 63 Ag 8653 myeloma cells. A clone (FDP 14) was selected that produces monoclonal...
article 1986
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TNO Gaubius Institute for Cardiovascular Research, 2313 AD Leiden, Netherlands (author), Rijken, D.C. (author), Groeneveld, E. (author)
Two-chain tissue-type plasminogen activator (t-PA), which consists of a heavy chain (M(r) ??? 38,000) and a light chain (M(r) ??? 31,000) connected by a disulfide bridge, was reduced with 2-mercaptoethanol and then air-reoxidized at a low protein concentration and carboxamidomethylated. The two chains were separated by means of zinc chelate...
article 1986
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Kluft, C. (author), Verheijen, J.H. (author), Jie, A.F.H. (author), Rijken, D.C. (author), Preston, F.E. (author), Sue-Ling, H.M. (author), Jespersen, J. (author), Aasen, A.O. (author), Gezondheidsorganisatie TNO Gaubius Instituut TNO (author)
The plasma activity level of the recently discovered fast-acting inhibitor of tissue-type plasminogen activator (t-PA) was found to be temporarily increased after surgery, myocardial infarction and severe trauma. Detailed analysis of the postoperative period revealed simultaneously increased t-PA antigen and inhibition and decreased t-PA...
article 1985
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D'Angelo, A. (author), Kluft, C. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)
In nine patients with non-malignant diseases undergoing major upper abdominal surgery, the mechanism of the postoperative fibrinolytic shut-down was investigated because of its potential significance for postoperative deep vein thrombosis by employing new and specific methods for assessing and stimulating the fibrinolytic system. The shut-down...
article 1985
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Emeis, J.J. (author), Kluft, C. (author), Gaubius instituut TNO (author)
Platelet-activating factor (PAF-acether; 1-O-octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) induced the release of plasminogen activator in rat, both in vivo and in perfused hind legs. The released plasminogen activator was shown by immunologic and functional criteria to be tissue-type plasminogen activator (t-PA). Release of t-PA by PAF...
article 1985
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Haverkate, F. (author), Nieuwenhuizen, W. (author), Gaubius Instituut TNO (author)
Chemicals/CAS: calcium chloride, 10043-52-4; calcium, 7440-70-2; citric acid, 126-44-3, 5949-29-1, 77-92-9, 8002-14-0; edetic acid, 150-43-6, 60-00-4
article 1984
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Kluft, C. (author), Vellenga, E. (author), Brommer, E.J.P. (author), Wijngaards, G. (author), Gaubius instituut TNO (author)
This study concerns a case of congenital homozygous deficiency in ??2-antiplasmin associated with a severe hemorrhagic diathesis. Heterozygous family members also show a mild bleeding tendency. The propositus is a 17-yr-old male born of white parents and showing a severe hemorrhagic diathesis characterized by spontaneous bleeding in the joints...
article 1982
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Kluft, C. (author), Traas, D.W. (author), Jie, A.F.H. (author), Hoegee de Nobel, E. (author), Gaubius instituut TNO (author)
Various plasmin preparations were tested for their suitability for use in the assay of ??2-antiplasmin in blood plasma by the immediate plasmin inhibition test. Activation of plasminogen, viz., 1-Glu-plasminogen and/or 77-Lys-plasminogen, by immobilized urokinase results in plasmin preparations suitable for this ??2-antiplasmin test. Plasmins...
article 1982
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Brommer, E.J.P. (author), Leuven, J.A.G. (author), Barrett-Bergshoeff, M.M. (author), Schouten, J.A. (author), Gaubius instituut TNO (author)
Impairment of fibrinolysis is supposed to contribute to CVD. In 38 hyperlipoproteinemic patients, known to be at risk for early CVD, fibrinolytic activity was measured before and after stimulation with DDAVP. A negative correlation was found between serum triglyceride levels and fibrinolytic activity, both before and after DDAVP. A subnormal...
article 1982
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Jenks, R.L. (author), Kluft, C. (author), Astrup, T. (author), Gezondheidsorganisatie TNO Gaubius Instituut TNO (author)
Bovine plasma yields fibrinolytically inactive euglobulin fractions, even when prepared in the presence of dextran sulphate. Addition of flufenamate to these solutions only occasionally elicits a slight activity. However, highly fibrinolytic solutions are produced when euglobulins precipitated in the presence of dextran sulphate are exposed to...
article 1981
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van der Sande, J.J. (author), Emeis, J.J. (author), Lindeman, J. (author), Gezondheidsorganisatie TNO Gaubius Instituut TNO (author)
Fibrin microthrombi were demonstrated by an immunoenzymehistochemical method in the small blood vessels of the lung and, to a lesser extent, in the brain in rats after minor experimental head injury. It was concluded that intravascular coagulation is a common phenomenon in head injury.
article 1981
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Jenks, R.L. (author), Astrup, T. (author), Gaubius instituut TNO (author)
Treatment of human citrated plasma with glass has complex effects on the fibrinolytic system. While the spontaneous euglobulin activity is only slightly affected by the glass treatment, the activity precipitated in the presence of dextran sulphate diminishes rapidly with increasing amounts of glass. With common glass a minimum is reached, and...
article 1980
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Kluft, C. (author), Gezondheidsorganisatie TNO Gaubius Instituut TNO (author)
Factor XII-prekallikrein dependent processes contribute to the fibrinolytic activity of plasma, Evidence for the occurrence of a plasminogen proactivator separate from factor XII, factor XI and prekallikrein is summarized.
conference paper 1980
document
Kluft, C. (author), Michiels, J.J. (author), Wijngaards, G. (author), Gaubius instituut TNO (author)
3 patients with Behcet's disease were studied for their fibrinolytic status during exacerbation of the disease accompanied with thrombotic complications. All 3 patients exhibited low euglobulin fibrinolytic activity. This, however, could be attributed to an artificially increased coprecipitation of inhibitors, i.e., C1-inactivator in the...
article 1980
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Guinan, M.E. (author), Astrup, T. (author), Gaubius instituut TNO (author)
The arteries of the rat usually possess considerably higher endothelium-related fibrinolytic activity than corresponding veins. This finding contrasts observations in most other species, including man. The use of the rat in experimental work on thrombotic vascular disease should be evaluated with due regard to the specific properties of the...
article 1980
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Smokovitis, A. (author), Astrup, T. (author), Gaubius instituut TNO (author)
Rabbit skin, normally of low fibrinolytic activity, and guinea-pig skin, of medium activity, after crush injury or after the intradermal or subcutaneous injection of aluminum hydroxide, show a remarkable increase in fibrinolytic activity. The activity is caused by a plasminogen activator and is related to blood vessels. Occasionally, intense...
article 1980
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Kluft, C. (author), Trumpi-Kalshoven, M.M. (author), Jie, A.F.H. (author), Veldhuyzen-Stolk, E.C. (author), Gaubius instituut TNO (author)
Fibrinolytic studies in euglobulin fractions of Fletcher trait plasma (deficient in prekallikrein) revealed reduced activities as compared to normal plasma. A quantitative assay for total plasminogen activator plus proactivator in plasma showed that the amount in Fletcher trait patients is about half of normal (normal = ?? 100 blood activator...
article 1979
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Smokovitis, A. (author), Astrup, T. (author), Gaubius instituut TNO (author)
Fibrinolytic activity (caused by a plasminogen activator) in the spinal cord was highest in the rat, lowest in the rabbit, and intermediate in the guinea pig. In all species the activity was highest in relation to the pia mater. The central spinal canal was active in the rat and the rabbit, but mostly inactive in the guinea pig. Foci of activity...
article 1978
Searched for: subject%3A%22fibrinolysis%22
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