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Gaubius Instituut TNO (author), van Hinsbergh, V.W.M. (author)Chemicals/CAS: Cytokines; Plasminogen Activator Inhibitor 1; plasminogen activator, urokinase receptors; Plasminogen, 9001-91-6; Receptors, Cell Surface; Urinary Plasminogen Activator, EC 3.4.21.73.article 1992
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Gaubius Instituut TNO (author), Kluft, C. (author)Chemicals/CAS: Biological Markers; Plasminogen Activator Inhibitor 1; Tissue Plasminogen Activator, EC 3.4.21.68.article 1992
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de Fouw, N.J. (author), Haverkate, F. (author), Bertina, R.M. (author), Gaubius Instituut TNO (author)The effect of purifed human activated protein C (APC) on fibrinolysis was studied by using in vitro clot lysis techniques. Clots were formed from citrated blood or plasma (supplemented with 125I-labeled fibrinogen) by adding thrombin and Ca2+-ions; lysis of the clots was achieved by the addition of tissue-type plasminogen activator before clot...article 1991
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Kooistra, T. (author), Opdenberg, J.P. (author), Toet, K. (author), Hendriks, H.F.J. (author), van den Hoogen, R.M. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)Tissue-type plasminogen activator (t-PA) and its inhibitor, plasminogen activator inhibitor 1 (PAI-1), play an important role in regulating the fibrinolytic capacity of plasma. Both t-PA and PAI-1 are synthesized by the endothelium. We report that retinoic acid (vitamin A acid) and other retinoids rather specifically stimulate the production of...article 1991
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Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author), de Munk, G.A.W. (author), Groeneveld, E. (author), Rijken, D.C. (author)The in vitro effects of thrombomodulin on the inactivation of single chain urokinase-type plasminogen activator (scu-PA) by thrombin were investigated by incubating scu-PA with varying concentrations of human thrombin, in both the absence and presence of soluble rabbit thrombomodulin. 50% inactivation of scu-PA occurred in 45 min at 160 ng/ml...article 1991
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van Hinsbergh, V.W.M. (author), Bauer, K.A. (author), Kooistra, T. (author), Kluft, C. (author), Dooijewaard, G. (author), Sherman, M.L. (author), Nieuwenhuizen, W. (author), Gaubius instituut TNO (author)Several investigators have reported that tumor necrosis factor (TNF) can alter the production of plasminogen activator type-1 (PAI-1) and plasminogen activators (PAs) by endothelial cells in vitro. We have examined the in vivo effects of recombinant human TNF administration on fibrinolysis as assessed by parameters in plasma during a 24-hour...article 1990
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van Hinsbergh, V.W.M. (author), Kooistra, T. (author), Scheffer, M.A. (author), van Bockel, J.H. (author), van Muijen, G.N.P. (author), Gaubius Instituut TNO (author)It has been reported that omental fat tissue is a good source of human microvascular endothelial cells. By characterization we demonstrate that the epithelioid cells isolated from omental tissue are not endothelial cells, but mesothelial cells. They contain abundant cytokeratins 8 and 18, which are absent in endothelial cells, and vimentin. No...article 1990
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Gaubius Instituut TNO (author), Wasser, M.N.J.M. (author), Pauwels, E.K.J. (author), Nieuwenhuizen, W. (author)This paper describes the production and characterization of an antifibrin monoclonal antibody, produced by immunizing mice with fibrinogen degradation fragment by the antibody (designated Y22) is directed against a conformation dependent epitope in the D-domain of fibrin Y22 was labeled with the radionuclide Tc-99m. Experiments in vitro and in...article 1990
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Tranquille, N. (author), Emeis, J.J. (author), Gaubius Instituut TNO (author)The acute release of tissue-type plasminogen activator (t-PA) was studied in perfused rat hindlegs. Pretreatment of rats with the protein synthesis inhibitor cycloheximide (2 mg/kg) at 1, 3 or 5 h prior to perfusion of rat hindlegs did not influence the amount of t-PA released by platelet-activating factor (20 nM) or bradykinin (1 μM). The...article 1989
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Emeis, J.J. (author), van Houwelingen, A.C. (author), van den Hoogen, C.M. (author), Hornstra, G. (author), Gaubius Institute TNO, 2300 AP Leiden, Netherlands (author)For a period of 6 weeks, 76 healthy male volunteers consumed during their daily main meal the contents of one tin (~ 135 g) of either fish (mackerel) paste or meat paste. Fibrinolytic parameters were determined in plasma samples obtained at the beginning and at the end of the experimental period. No changes were found in plasminogen, α2...article 1989
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Brommer, E.J.P. (author), Derkx, F.H.M. (author), Schalekamp, M.A.D.H. (author), Dooijewaard, G. (author), Klaauw, V.D.M.M. (author), Gaubius instituut TNO (author)In eight male patients with normal liver and kidney function fibrinolytic components were measured in arterial blood and in renal and hepatic vein blood, obtained during catheterization for analysis of hypertension. Blood samples were collected simultaneously from veins und corresponding arteries before and 5 minutes after the completion of...article 1988
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Emeis, J.J. (author), Edgell, C.J.S. (author), Gaubius instituut TNO (author)The fibrinolytic characteristics of the endothelial hybrid cell line EA.hy 926, established by fusing a human umbilical vein endothelial cell with a human carcinoma cell line, were studied. The hybrid cell line produced large amounts of tissue-type plasminogen activator (t-PA), plasminogen activator inhibitor type 1, and a small amount of...article 1988
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Gaubius instituut TNO (author), Kluft, C. (author)Fibrinolytic agents used in intravenous thrombolytic therapy of myocardial infarction also exert, at effective dosages, significant side effects on the haemostatic system outside the direct resolution of the target thrombus. The side effects of the agents streptokinase and anisoylated plasminogen-streptokinase activator complex are larger than...article 1988
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Gaubius instituut TNO (author), Nieuwenhuizen, W. (author)The presence of a particular fibrinogen derivative in the circulation can be characteristic of a particular ongoing process: soluble fibrin can be used as a marker for activated coagulation, fibrinogen degradation products for fibrinogenolysis, and crosslinked fibrin degradation products for fibrinolysis. Fibrin(ogen) degradation products are...article 1988
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Pikaar, N.A. (author), Wedel, M. (author), van der Beek, E.J. (author), van Dokkum, W. (author), Kempen, H.J.M. (author), Kluft, C. (author), Ockhuizen, T. (author), Hermus, R.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)To investigate the effect of moderate alcohol consumption on blood constituents related to cardiovascular disease, 12 male volunteers consumed (instead of their usual alcoholic drinks) four different standardized amounts of red wine in addition to their habitual diet. Each dose was given to the subjects during a period of 5 weeks in a randomized...article 1987
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Kluft, C. (author), Nieuwenhuis, H.K. (author), Rijken, D.C. (author), Groeneveld, E. (author), Wijngaards, G. (author), van Berkel, W. (author), Dooijewaard, G. (author), Sixma, J.J. (author), Gaubius instituut TNO (author)??2-Antiplasmin (??2-AP) is a major fibrinolysis inhibitor, whose complete, congenital absence has been found to be associated with a distinct hemorrhagic diathesis. We studied a 15-yr-old male with a hemorrhagic diathesis after trauma from early childhood on. This bleeding tendency was associated with a minimal ??2-AP level recorded...article 1987
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Sprengers, E.D. (author), Kluft, C. (author), Gaubius Instituut TNO (author)Chemicals/CAS: fibrin, 9001-31-4; plasminogen activator inhibitor, 105844-41-5; plasminogen activator, 9039-53-6; Carrier Proteins; Glycoproteins; Plasminogen Activators, EC 3.4.21.-; Plasminogen Inactivators; Protease Inhibitors; protease-nexinarticle 1987
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Gaubius instituut TNO (author), Engesser, L. (author), Kluft, C. (author), Briet, E. (author), Brommer, E.J.P. (author)A patient with spontaneous venous thrombotic events, myocardial infarction and a positive family history of thrombosis was investigated for underlying disorders of haemostasis. Abnormally high levels of histidine-rich glycoprotein were found. No other abnormality known or suspected of increasing the risk for thrombosis was present. Elevated...article 1987
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de Fouw, N.J. (author), Haverkate, F. (author), Bertina, R.M. (author), Gaubius Instituut TNO (author)The effect of purified human activated protein C (APC) and protein S on fibrinolysis was studied by using an in vitro blood clot lysis technique. Blood clots were formed from citrated blood (supplemented with 125I-fibrinogen) by adding thrombin and Ca2+-ions; lysis of the clots was achieved by adding tissue-type plasminogen activator. The...article 1986
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Gaubius instituut TNO (author), Boks, A.L. (author), Brommer, E.J.P. (author), Schalm, S.W. (author), van Vliet, H.H.D.M. (author)In a study of severe, decompensated liver failure, we tried to find a correlation between hemorrhage and parameters of hemostasis and fibrinolysis. Three groups of patients were studied: alcoholic cirrhosis; nonalcoholic cirrhosis, and acute liver failure without known prior liver disease. The two cirrhotic groups did not differ significantly...article 1986