Searched for: subject%3A%22extracellular%255C%2Bmatrix%22
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Kroon, M.E. (author), Koolwijk, P. (author), van der Vecht, B. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Hypoxia stimulates angiogenesis, the formation of new blood vessels. This study evaluates the direct effect of hypoxia (1% oxygen) on the angiogenic response of human microvascular endothelial cells (hMVECs) seeded on top of a 3-dimensional fibrin matrix, hMVECs stimulated with fibroblast growth factor-2 (FGF-2) or vascular endothelial growth...
article 2000
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Myrup, B. (author), Rossing, P. (author), Jensen, T. (author), Gram, J. (author), Kluft, C. (author), Jespersen, J. (author), Gaubius instituut TNO (author)
Objective: To investigate protein concentration changes during venous occlusion of proteins with reported affinity for extracellular matrix (plasminogen activator inhibitor type 1, antithrombin III, fibronectin and von Willebrand factor) in comparison with proteins with no reported affinity (albumin, tissue-type plasminogen activator,...
article 1999
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de Groot, J. (author), Verzijl, N. (author), Bank, R.A. (author), Lafeber, F.P.J.G. (author), Bijlsma, J.W.J. (author), TeKoppele, J.M. (author)
Objective. To examine the effect of nonenzymatic glycation of cartilage extracellular matrix on the synthetic activity of chondrocytes. Methods. The proteoglycan-synthesis rate (35SO42- incorporation) and levels of advanced nonenzymatic glycation (determined by high-performance liquid chromatography measurement of pentosidine) were evaluated in...
article 1999
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Lansink, M. (author), Koolwijk, P. (author), van Hinsbergh, V. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)
Angiogenesis, the formation of new capillary blood vessels, is a feature of a variety of pathological processes. To study the effects of a specific group of hormones (all ligands of the steroid/retinoid/thyroid hormone receptor superfamily) on the angiogenic process in humans, we have used a model system in which human microvascular endothelial...
article 1998
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van der Kaaden, M.E. (author), Rijken, D.C. (author), van Berkel, T.J.C. (author), Kuiper, J. (author), Gaubius Instituut TNO (author)
article 1998
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Basser, P.J. (author), Schneiderman, R. (author), Bank, R.A. (author), Wachtel, E. (author), Maroudas, A. (author), TNO Preventie en Gezondheid (author)
We have used an isotropic osmotic stress technique to assess the swelling pressures of human articular cartilage over a wide range of hydrations in order to determine from these measurements, for the first time, the tensile stress in the collagen network, P(c), as a function of hydration. Osmotic stress was applied by means of calibrated...
article 1998
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Beekman, B. (author), Verzijl, N. (author), de Roos, J.A.D.M. (author), TeKoppele, J.M. (author), Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author)
Objective: To determine the role of interleukin-1β (IL-1β) in the degradation of proteoglycans and collagen by articular chondrocytes. Design: Chondrocytes were cultured in alginate beads for 2 weeks to produce extracellular matrix, followed by the addition of IL-1β for 1 or 2 days. Breakdown of extracellular matrix (with and without activation...
article 1998
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Beekman, B. (author), Verzijl, N. (author), Bank, R.A. (author), Von Der Mark, K. (author), TeKoppele, J.M. (author), TNO Preventie en Gezondheid (author)
The extracellular matrix synthesized by articular chondrocytes cultured in alginate beads was investigated. Collagen levels increased sigmoidally with time and remained constant after 2 weeks of culture. The presence of cartilage-specific type II collagen was confirmed immunohistochemically. Predominantly type H collagen was present in the...
article 1997
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Gaubius Instituut TNO (author), Verheijen, J.H. (author), Nieuwenbroek, N.M.E. (author), Beekman, B. (author), Hanemaaijer, R. (author), Verspaget, H.W. (author), Ronday, K.H. (author), Bakker, A.H.F. (author)
We describe a new principle for assessment of the activity of proteolytic enzymes of all classes and show the application of this principle for the quantitative assay of bacterial collagenase and human matrix metalloproteinases (MMPs). Central to this new principle is the presence of a proenzyme that can be activated into an active enzyme by a...
article 1997
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Meulenbelt, I. (author), Bijkerk, C. (author), de Wildt, S.C.M. (author), Miedema, H.S. (author), Valkenburg, H.A. (author), Breedveld, F.C. (author), Pols, H.A.P. (author), TeKoppele, J.M. (author), Sloos, V.F.G. (author), Hofman, A. (author), Slagboom, P.E. (author), van Duijn, C.M. (author)
Objective. To investigate whether radiographically evident osteoarthritis (ROA) in 55-65-year-old men and women is associated with specific alleles or genotypes of the cartilage matrix protein (CRTM) and cartilage link protein (CRTL1) genes. Methods. Cases were selected from a population-based study on the presence of ROA of the knee or hip....
article 1997
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de Bart, A.C.W. (author), Quax, P.H.A. (author), Löwik, C.W.G.M. (author), Verheijen, J.H. (author), TNO Preventie en Gezondheid (author)
Plasmin-mediated extracellular proteolysis has been implicated in the degradation of bone in normal and pathological conditions. Normal and malignant osteoblasts can produce both tissue-type plasminogen activator (t- PA) and urokinase-type plasminogen activator (u-PA). We have used the osteosarcoma cell line MG63 to address the question of...
article 1995
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Gaubius Instituut TNO (author), Hanemaaijer, R. (author), Koolwijk, P. (author), Le Clercq, L. (author), de Vree, W.J.A. (author), van Hinsbergh, V.W.M. (author)
Matrix metalloproteinases (MMPs) play a role in tissue remodelling and angiogenesis. We have investigated the expression and regulation of MMP-1 (interstitial collagenase), MMP-2 (gelatinase A), MMP-3 (stromelysin 1), MMP-7 (matrilysin), MMP-9 (gelatinase B) and their inhibitors TIMP-1 and TIMP-2 in human umbilical vein, femoral vein and...
article 1993
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