Searched for: subject:"enzyme%5C+inhibitor"
(1 - 20 of 49)

Pages

document
Ceconi, C. (author), Fox, K.M. (author), Remme, W.J. (author), Simoons, M.L. (author), Deckers, J.W. (author), Bertrand, M. (author), Parrinello, G. (author), Kluft, C. (author), Blann, A. (author), Cokkinos, D. (author), Ferrari, R. (author), TNO Kwaliteit van Leven (author)
The PERTINENT study measured biomarkers of atherosclerosis and thrombosis in a stable coronary artery disease population from EUROPA receiving ACE inhibition with perindopril 8 mg/day or placebo. Biomarkers of inflammation, C-reactive protein (CRP), fibrinogen, and tumor necrosis factor-alpha (TNF-α), and a biomarker of thrombosis, d-dimer, were...
article 2009
document
Lindeman, J.H.N. (author), Abdul-Hussien, H. (author), van Bockel, J.H. (author), Wolterbeek, R. (author), Kleemann, R. (author), TNO Kwaliteit van Leven (author)
Background-Doxycycline has been shown to effectively inhibit aneurysm formation in animal models of abdominal aortic aneurysm. Although this effect is ascribed to matrix metalloproteinase-9 inhibition, such an effect is unclear in human studies. We reevaluated the effect of doxycycline on aortic wall protease content in a inical trial and found...
article 2009
document
Jeurissen, S.M.F. (author), Punt, A. (author), Delatour, T. (author), Rietjens, I.M.C.M. (author), TNO Kwaliteit van Leven (author)
The effects of a basil extract on the sulfation and concomitant DNA adduct formation of the proximate carcinogen 1′-hydroxyestragole were studied using rat and human liver S9 homogenates and the human hepatoma cell line HepG2. Basil was chosen since it contains the procarcinogen estragole that can be metabolized to 1′-hydroxyestragole by...
article 2008
document
Tchetverikov, I. (author), Kraan, M.C. (author), van El, B. (author), Hanemaaijer, R. (author), de Groot, J. (author), Huizinga, T.W.J. (author)
Objective: To analyse the effects of leflunomide and methotrexate treatment on matrix metalloproteinase (MMP) activity levels in a2 macroglobulin/MMP (α2M/MMP) complexes in the systemic circulation of rheumatoid arthritis (RA) patients. Methods: A total of 102 RA patients from a prospective, double-blind, randomised clinical trial comparing...
article 2008
document
Fabriek, B.O. (author), Møller, H.J. (author), Vloet, R.P.M. (author), van Winsen, L.M. (author), Hanemaaijer, R. (author), Teunissen, C.E. (author), Uitdehaag, B.M.J. (author), van den Berg, T.K. (author), Dijkstra, C.D. (author), TNO Kwaliteit van Leven (author)
The scavenger receptor CD163 is selectively expressed on tissue macrophages and human monocytes. CD163 has been implicated to play a role in the clearance of hemoglobin and in the regulation of cytokine production by macrophages. Membrane CD163 can be cleaved by matrix metalloproteinases (MMP) resulting in soluble CD163 (sCD163). In the present...
article 2007
document
Jeurissen, S.M.F. (author), Claassen, F.W. (author), Havlik, J. (author), Bouwmans, E.E. (author), Cnubben, N.H.P. (author), Sudhölter, E.J.R. (author), Rietjens, I.M.C.M. (author), van Beek, T.A. (author), TNO Kwaliteit van Leven (author)
An on-line HPLC screening method for detection of inhibitors of human cytochrome P450 1A2 in extracts was developed. HPLC separation of extracts is connected to a continuous methoxyresorufin-O-demethylation (MROD) assay in which recombinant human P450 1A2 converts methoxyresorufin to its fluorescent metabolite resorufin. The system was tested...
article 2007
document
Jeurissen, S.M.F. (author), Punt, A. (author), Boersma, M.G. (author), Bogaards, J.J.P. (author), Fiamegos, Y.C. (author), Schilter, B. (author), van Bladeren, P.J. (author), Cnubben, N.H.P. (author), Rietjens, I.M.C.M. (author), TNO Kwaliteit van Leven (author)
Human cytochrome P450 enzymes involved in the bioactivation of estragole to its proximate carcinogen 1′-hydroxyestragole were identified and compared to the enzymes of importance for 1′-hydroxylation of the related alkenylbenzenes methyleugenol and safrole. Incubations with Supersomes revealed that all enzymes tested, except P450 2C8, are...
article 2007
document
Houben, E. (author), Uchida, Y. (author), Nieuwenhuizen, W.F. (author), de Paepe, K. (author), Vanhaecke, T. (author), Holleran, W.M. (author), Rogiers, V. (author), TNO Kwaliteit van Leven (author)
It has recently become evident that at least five ceramidase (CDase) isoforms are present in human epidermis, and that specifically acidic CDase (aCDase) and alkaline CDase (alkCDase) activities increase during keratinocyte differentiation, and thus might play a pivotal role(s) in permeability barrier function. Prior to investigating their...
article 2007
document
Korsheninnikova, E. (author), van der Zon, G.C.M. (author), Voshol, P.J. (author), Janssen, G.M. (author), Havekes, L.M. (author), Grefhorst, A. (author), Kuipers, F. (author), Reijngoud, D.-J. (author), Romijn, J.A. (author), Ouwens, D.M. (author), Maassen, J.A. (author), TNO Kwaliteit van Leven (author)
Aims/hypothesis: Activation of nutrient sensing through mammalian target of rapamycin (mTOR) has been linked to the pathogenesis of insulin resistance. We examined activation of mTOR-signalling in relation to insulin resistance and hepatic steatosis in mice. Materials and methods: Chronic hepatic steatosis and hepatic insulin resistance were...
article 2006
document
Jeurissen, S.M.F. (author), Bogaards, J.J.P. (author), Boersma, M.G. (author), ter Horst, J.P.F. (author), Awad, H.M. (author), Fiamegos, Y.C. (author), van Beek, T.A. (author), Alink, G.M. (author), Sudhölter, E.J.R. (author), Cnubben, N.H.P. (author), Rietjens, I.M.C.M. (author), TNO Kwaliteit van Leven (author)
In vitro studies were performed to elucidate the human cytochrome P450 enzymes involved in the bioactivation of methyleugenol to its proximate carcinogen 1′-hydroxymethyleugenol. Incubations with Supersomes, expressing individual P450 enzymes to a high level, revealed that P450 1A2, 2A6, 2C9, 2C19, and 2D6 are intrinsically able to 1′...
article 2006
document
Bildt, M.M. (author), Snoek-van Beurden, A.M.P. (author), de Groot, J. (author), van El, B. (author), Kuijpers-Jagtman, A.M. (author), van den Hoff, J.W. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Background and Objective: The purpose of this study was to investigate the effects of chemically modified tetracyclines (CMTs) on the production of gelatinases [matrix metalloproteinase (MMP)-2 and -9] by human periodontal ligament (PDL) cells, and on the activity of recombinant gelatinases. Material and Methods: Human PDL cells were cultured...
article 2006
document
Lund, S. (author), Porzgen, P. (author), Mortensen, A.L. (author), Hasseldam, H. (author), Bozyczko-Coyne, D. (author), Morath, S. (author), Hartung, T. (author), Bianchi, M. (author), Ghezzi, P. (author), Bsibsi, M. (author), Dijkstra, S. (author), Leist, M. (author), TNO Preventie en Gezondheid (author)
CEP-1347 is a potent inhibitor of the mixed lineage kinases (MLKs), a distinct family of mitogen-activated protein kinase kinase kinases (MAPKKK). It blocks the activation of the c-Jun/JNK apoptotic pathway in neurons exposed to various stressors and attenuates neurodegeneration in animal models of Parkinson's disease (PD). Microglial activation...
article 2005
document
Caspary, F. (author), Elliott, G. (author), Navé, B.T. (author), Verzaal, P. (author), Rohrbach, M. (author), Das, P.K. (author), Nagelkerken, L. (author), Nieland, J.D. (author), Gaubius Instituut TNO (author)
Background: The dogma in psoriasis is that due to pathogen-induced inflammatory responses, an autoreactive immune response is induced that leads to tissue destruction. However, this model might be too simplistic. Literature data suggest that the expression of enzymes crucial for fatty acid oxidation is upregulated in the skin of patients with...
article 2005
document
Nelson, G. (author), Kozlova-Zwinderman, O. (author), Collis, A.J. (author), Knight, M.R. (author), Fincham, J.R.S. (author), Stanger, C.P. (author), Renwick, A. (author), Hessing, J.G.M. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Read, N.D. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Calcium signalling is little understood in filamentous fungi largely because easy and routine methods for calcium measurement in living hyphae have previously been unavailable. We have developed the recombinant aequorin method for this purpose. High levels of aequorin expression were obtained in Neurospora crassa, Aspergillus niger and...
article 2004
document
Sengupta, S. (author), Toh, S.A. (author), Sellers, L.A. (author), Skepper, J.N. (author), Koolwijk, P. (author), Leung, H.W. (author), Yeung, H.W. (author), Wong, R.N.S. (author), Sasisekharan, R. (author), Fan, T.P.D. (author)
Background-Ginseng is a commonly used nutraceutical. Intriguingly, existing literature reports both wound-healing and antitumor effects of ginseng extract through opposing activities on the vascular system. To elucidate this perplexity, we merged a chemical fingerprinting approach with a deconstructional study of the effects of pure molecules...
article 2004
document
de Wijk, R.A. (author), Prinz, J.F. (author), Engelen, L. (author), Weenen, H. (author), TNO Voeding (author)
The role of salivary α-amylase in odour, flavour, and oral texture sensations was investigated in two studies in which the activity of salivary amylase present in the mouth of human subjects was either increased by presenting custards with added α-amylase or decreased by presenting custards with added acarbose, an amylase inhibitor. For starch...
article 2004
document
van Nieuw Amerongen, G.P. (author), Natarajan, K. (author), Yin, G. (author), Hoefen, R.J. (author), Osawa, M. (author), Haendeler, J. (author), Ridley, A.J. (author), Fujiwara, K. (author), van Hinsbergh, V.W.M. (author), Berk, B.C. (author), Gaubius Instituut TNO (author)
Thrombin mediates changes in endothelial barrier function and increases endothelial permeability. A feature of thrombin-enhanced endothelial hyperpermeability is contraction of endothelial cells (ECs), accompanied by formation of focal adhesions (FAs). Recently, a G protein-coupled receptor kinase-interacting protein, GIT1, was shown to regulate...
article 2004
document
van Hensbergen, Y. (author), Broxterman, H.J. (author), Peters, E. (author), Rana, S. (author), Elderkamp, Y.W. (author), van Hinsbergh, V.W.M. (author), Koolwijk, P. (author), Gaubius Instituut TNO (author)
The aminopeptidase inhibitor bestatin has been shown to have anti-angiogenic effects in a number of model systems. These effects are thought to result from inhibition of CD13 activity. Because tumor angiogenesis can evolve in a fibrin-rich stroma matrix we have studied for the first time the effects of bestatin on microvascular endothelial...
article 2003
document
Walker, J.B. (author), Hughes, B. (author), James, I. (author), Haddock, P. (author), Kluft, C. (author), Bajzar, L. (author), Gaubius Instituut TNO (author)
Two competitive inhibitors of TAFIa (activated thrombin-activable fibrinolysis inhibitor), 2-guanidinoethyl-mercaptosuccinic acid and potato tuber carboxypeptidase inhibitor, variably affect fibrinolysis of clotted human plasma. Depending on their concentration, the inhibitors shortened, prolonged, or had no effect on lysis in vitro. The...
article 2003
document
van Nieuw Amerongen, G.P. (author), Koolwijk, P. (author), Versteilen, A. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Objective - Growth factor-induced angiogenesis involves migration of endothelial cells (ECs) into perivascular areas and requires active remodeling of the endothelial F-actin cytoskeleton. The small GTPase RhoA previously has been implicated in vascular endothelial growth factor (VEGF)-induced signaling pathways, but its role has not been...
article 2003
Searched for: subject:"enzyme%5C+inhibitor"
(1 - 20 of 49)

Pages