Searched for: subject%3A%22cytoskeleton%22
(1 - 10 of 10)
document
Heinzmann, A.C.A. (author), Karel, M.F.A. (author), Coenen, D.M. (author), Vajen, T. (author), Meulendijks, N.M.M. (author), Nagy, M. (author), Suylen, D.P.L. (author), Cosemans, J.M.E.M. (author), Heemskerk, J.W.M. (author), Hackeng, T.M. (author), Koenen, R.R. (author)
Background and aims:Platelets can release extracellular vesicles (EVs) upon stimulation with various agonists.Interestingly, platelets from patients with Glanzmann thrombasthenia have reduced EV release. These plateletslack functional αIIbβ3integrins, indicating that αIIbβ3integrin is critical in vesicle release. Integrin activation iscentral in...
article 2020
document
Zakrzewska, A. (author), Boorsma, A. (author), Beek, A.T. (author), Hageman, J.A. (author), Westerhuis, J.A. (author), Hellingwerf, K.J. (author), Brul, S. (author), Klis, F.M. (author), Smits, G.J. (author), TNO Kwaliteit van Leven (author)
The transcriptional responses of yeast cells to a wide variety of stress conditions have been studied extensively. In addition, deletion mutant collections have been widely used to measure the combined effect of gene loss and stress on growth (fitness). Here we present a comparative analysis of 1,095 publicly available transcription and genome...
article 2010
document
Heijne, W.H.M. (author), Slitt, A.L. (author), van Bladeren, P.J. (author), Groten, J.P. (author), Klaassen, C.D. (author), Stierum, R.H. (author), van Ommen, B. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Rats were exposed to three levels of bromobenzene, sampled at 6, 24, and 48 h, and liver gene expression profiles were determined to identify dose and time-related changes. Expression of many genes changed transiently, and dependent on the dose. Few changes were identified after 6 h, but many genes were differentially expressed after 24 h, while...
article 2004
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Stierum, R. (author), Gaspari, M. (author), Dommels, Y. (author), Ouatas, T. (author), Pluk, H. (author), Jespersen, S. (author), Vogels, J. (author), Verhoeckx, K. (author), Groten, J. (author), van Ommen, B. (author)
Here, we describe a proteomics approach to study protein expression changes in differentiating Caco-2 cells. Caco-2 is a colorectal carcinoma cell line, which upon differentiation loses its tumorigenic phenotype and displays characteristics of mature enterocytes, including brush borders with microvilli. Cells were grown in culture flasks and...
article 2003
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van Nieuw Amerongen, G.P. (author), Koolwijk, P. (author), Versteilen, A. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Objective - Growth factor-induced angiogenesis involves migration of endothelial cells (ECs) into perivascular areas and requires active remodeling of the endothelial F-actin cytoskeleton. The small GTPase RhoA previously has been implicated in vascular endothelial growth factor (VEGF)-induced signaling pathways, but its role has not been...
article 2003
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van Nieuw Amerongen, G.P. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Rho-like small GTPases, with their main representatives (Rho, Rac, and Cdc42), have been recognized in the past decade as key regulators of the F-actin cytoskeleton. Rho-like small GTPases are now known to play a major role in vascular processes caused by changes in the actin cytoskeleton, such as smooth muscle cell contraction, endothelial...
article 2001
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van Nieuw Amerongen, G.P. (author), Delft, S.V. (author), Vermeer, M.A. (author), Collard, J.G. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Endothelial cells (ECs) actively regulate the extravasation of blood constituents. On stimulation by vasoactive agents and thrombin, ECs change their cytoskeletal architecture and small gaps are formed between neighboring cells. These changes partly depend on a rise in [Ca2+](i) and activation of the Ca2+/calmodulin-dependent myosin light chain...
article 2000
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van Nieuw Amerongen, G.P. (author), Vermeer, M.A. (author), van Hinsbergh, V.W. (author), Gaubius Instituut TNO (author)
In the present study, the roles of the small GTPase RhoA and its target Rho kinase in endothelial permeability were investigated in vitro. We have shown previously that, in addition to a rise in the intracellular Ca(2+) concentration ([Ca(2+)](i)), RhoA is involved in the prolonged thrombin-induced barrier dysfunction. To study the role of RhoA...
article 2000
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van Nieuw Amerongen, G.P. (author), Draijer, R. (author), Vermeer, M.A. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
In the present study, we differentiated between short- and long-term effects of vasoactive compounds on human endothelial permeability in an in vitro model. Histamine induced a rapid and transient (<3 minutes) decrease in barrier function, as evidenced by a decreased transendothelial electrical resistance and an increased passage of 22Na ions....
article 1998
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Bruijnzeel, P.L.B. (author)
article 1994
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