Searched for: subject:"complementary%5C+DNA"
(1 - 20 of 31)

Pages

document
de Haan, W. (author), de Vries-van der Weij, J. (author), Mol, I.M. (author), Hoekstra, M. (author), Romijn, J.A. (author), Jukema, J.W. (author), Havekes, L.M. (author), Princen, H.M.G. (author), Rensen, P.C.N. (author), TNO Kwaliteit van Leven (author)
Pregnane X receptor (PXR) agonism has been shown to affect multiple steps in both the synthesis and catabolism of HDL, but its integrated effect on HDL metabolism in vivo remains unclear. The aim of this study was to evaluate the net effect of PXR agonism on HDL metabolism in ApoE*3-Leiden (E3L) and E3L.CETP mice, well-established models for...
article 2009
document
Plaisier, M. (author), Dennert, I. (author), Rost, E. (author), Koolwijk, P. (author), van Hinsbergh, V.W.M. (author), Helmerhorst, F.M. (author), TNO Kwaliteit van Leven (author)
BACKGROUND : Decidual vascular development is important for implantation. This study analysed decidual vascular adaptation to implantation in correlation with miscarriage in decidual secretory endometrium (DSE), decidua parietalis (DP) and decidua basalis (DB) of miscarriage patients and matched controls. METHODS : Decidua was obtained during...
article 2009
document
Staal, Y.C.M. (author), Pushparajah, D.S. (author), van Herwijnen, M.H.M. (author), Gottschalk, R.W.H. (author), Maas, L.M. (author), Ioannides, C. (author), van Schooten, F.J. (author), van Delft, J.H.M. (author), TNO Kwaliteit van Leven (author)
Although exposure to polycyclic aromatic hydrocarbons (PAHs) occurs mostly through mixtures, hazard and risk assessment are mostly based on the effects caused by individual compounds. The objective of the current study was to investigate whether interactions between PAHs occur, focusing on gene expression (as measured by cDNA microarrays) and...
article 2008
document
Engelse, M.A. (author), Laurens, N. (author), Verloop, R.E. (author), Koolwijk, P. (author), van Hinsbergh, V.W.M. (author), TNO Kwaliteit van Leven (author)
The formation of new tubular structures from a quiescent endothelial lining is one of the hallmarks of sprouting angiogenesis. This process can be mimicked in vitro by inducing capillary-like tubular structures in a three-dimensional (3D) fibrin matrix. We aimed to analyze the differential mRNA expression in two phenotypically distinct cell...
article 2008
document
Stierum, R. (author), Conesa, A. (author), Heijne, W. (author), Ommen, B.v. (author), Junker, K. (author), Scott, M.P. (author), Price, R.J. (author), Meredith, C. (author), Lake, B.G. (author), Groten, J. (author), TNO Kwaliteit van Leven (author), KvL (author)
Transcriptomics was performed to gain insight into mechanisms of food additives butylated hydroxytoluene (BHT), curcumin (CC), propyl gallate (PG), and thiabendazole (TB), additives for which interactions in the liver can not be excluded. Additives were administered in diets for 28 days to Sprague-Dawley rats and cDNA microarray experiments were...
article 2008
document
Wang, Y. (author), Xiao, Y. (author), Zhang, Y. (author), Chai, C. (author), Wei, G. (author), Wei, X. (author), Xu, H. (author), Wang, M. (author), Ouwerkerk, P.B.F. (author), Zhu, Z. (author), TNO Kwaliteit van Leven (author)
Monosaccharides transporters play important roles in assimilate supply for sink tissue development. In this study, a new monosaccharide transporter gene OsMST6 was identified from rice (Oryza sativa L.). The predicted OsMST6 protein shows typical features of sugar transporters and shares 79.6% identity with the rice monosaccharide transporter...
article 2008
document
Wang, Y. (author), Xu, H. (author), Wei, X. (author), Chai, C. (author), Xiao, Y. (author), Zhang, Y. (author), Chen, B. (author), Xiao, G. (author), Ouwerkerk, P.B.F. (author), Wang, M. (author), Zhu, Z. (author), TNO Kwaliteit van Leven (author)
Monosaccharide transporters mediate the membrane transport of a variable range of monosaccharides, which plays a crucial role in sugar distribution throughout the plant. To investigate the significance of monosaccharide transporters for rice (Oryza sativa L.) seed development, cDNA of a new putative monosaccharide transporter gene OsMST4 was...
article 2007
document
Hu, L. (author), Bovenschen, N. (author), Havekes, L.M. (author), van Vlijmen, B.J.M. (author), Tamsma, J.T. (author), TNO Kwaliteit van Leven (author)
article 2007
document
Bsibsi, M. (author), Bajramovic, J.J. (author), van Duijvenvoorden, E. (author), Persoon, C. (author), Ravid, R. (author), van Noort, J.M. (author), Vogt, M.H.J. (author), TNO Kwaliteit van Leven (author)
Human astrocytes express a limited repertoire of Toll-like receptor (TLR) family members including TLR1-4, which are expressed on the cell surface. Also, TLR3 but not TLR4 activation on astrocytes induces expression of several factors involved in neuroprotection and down-regulation of inflammation rather than in the onset of traditional pro...
article 2007
document
Meeuwsen, S. (author), Bsibsi, M. (author), Persoon-Deen, C. (author), Ravid, R. (author), van Noort, J.M. (author), TNO Preventie en Gezondheid (author)
Objectives: Brain microglia are highly responsive cells in the central nervous system that exert key functions in host defense as well as in neuroprotection and regeneration. In this study the gene expression profiles for 268 cytokines, chemokines, growth factors and their receptors were examined in cultures of purified human adult microglia,...
article 2005
document
van Erk, M.J. (author), Krul, C.A.M. (author), Caldenhoven, E. (author), Stierum, R.H. (author), Peters, W.H. (author), Woutersen, R.A. (author), van Ommen, B. (author), TNO Kwaliteit van Leven TNO Voeding (author)
Interest in mechanisms of colon cancer prevention by food compounds is strong and research in this area is often performed with cultured colon cancer cells. In order to assess utility for screening of potential cancer-preventive (food) compounds, expression profiles of 14 human cell lines derived from colonic tissue were measured using cDNA...
article 2005
document
te Biesebeke, R. (author), Levin, A. (author), Sagt, C. (author), Bartels, J. (author), Goosen, T. (author), Ram, A. (author), van den Hondel, C. (author), Punt, P. (author), TNO Kwaliteit van Leven (author)
Aspergillus oryzae requires polarized growth for colonization of solid substrates, and this growth phenotype differs from that seen in liquid medium. Various experimental approaches were used to identify genes that are differentially expressed when A. oryzae is grown on wheat kernels and in a wheat-based liquid medium. Hybridization of A. oryzae...
article 2005
document
Walker, L.C. (author), Overstreet, M.A. (author), Siddiqui, A. (author), de Paepe, A. (author), Ceylaner, G. (author), Malfait, F. (author), Symoens, S. (author), Atsawasuwan, P. (author), Yamauchi, M. (author), Ceylaner, S. (author), Bank, R.A. (author), Yeowell, H.N. (author), TNO Preventie en Gezondheid (author)
The clinical diagnosis of a patient with the phenotype of Ehlers-Danlos syndrome type VI was confirmed biochemically by the severely diminished level of lysyl hydroxylase (LH) activity in the patient's skin fibroblasts. A novel homozygous mutation, a single base change of T1360 → G in exon 13 of the LH1 gene, predicted to result in W446G, was...
article 2005
document
Verhoeckx, K.C.M. (author), Bijlsma, S. (author), de Groene, E.M. (author), Witkamp, R.F. (author), van der Greef, J. (author), Rodenburg, R.J.T. (author), TNO Voeding (author)
The monocyte-like human histiocytic lymphoma cell line U937 can be induced by phorbol 12-myristate 13-acetate (PMA) to undergo differentiation into a macrophage-like phenotype. We have used two-dimensional gel electrophoresis (2-DE), oligonucleotide microarrays and principal component analysis (PCA) to characterize the U937 cell line as a model...
article 2004
document
van Erk, M.J. (author), Teuling, E. (author), Staal, Y.C.M. (author), Huybers, S. (author), van Bladeren, P.J. (author), Aarts, J.M.M.J.G. (author), van Ommen, B. (author), TNO Voeding (author)
Background. Curcumin is a spice and a coloring food compound with a promising role in colon cancer prevention. Curcumin protects against development of colon tumors in rats treated with a colon carcinogen, in colon cancer cells curcumin can inhibit cell proliferation and induce apoptosis, it is an anti-oxidant and it can act as an anti...
article 2004
document
Wijnands, M.V.W. (author), van Erk, M.J. (author), Doornbos, R.P. (author), Krul, C.A.M. (author), Woutersen, R.A. (author)
The effects of different dietary compounds on the formation of aberrant crypt foci (ACF) and colorectal tumours and on the expression of a selection of genes were studied in rats. Azoxymethane-treated male F344 rats were fed either a control diet or a diet containing 10% wheat bran (WB), 0.2% curcumin (CUR), 4% rutin (RUT) or 0.04% benzyl...
article 2004
document
Heijne, W.H.M. (author), Slitt, A.L. (author), van Bladeren, P.J. (author), Groten, J.P. (author), Klaassen, C.D. (author), Stierum, R.H. (author), van Ommen, B. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Rats were exposed to three levels of bromobenzene, sampled at 6, 24, and 48 h, and liver gene expression profiles were determined to identify dose and time-related changes. Expression of many genes changed transiently, and dependent on the dose. Few changes were identified after 6 h, but many genes were differentially expressed after 24 h, while...
article 2004
document
Koppelman, S.J. (author), Knol, E.F. (author), Vlooswijk, R.A.A. (author), Wensing, M. (author), Knulst, A.C. (author), Hefle, S.L. (author), Gruppen, H. (author), Piersma, S. (author)
Background: Peanut allergen Ara h 3 has been the subject of investigation for the last few years. The reported data strongly depend on recombinant Ara h 3, since a purification protocol for Ara h 3 from peanuts was not available. Methods: Peanut allergen Ara h 3 (glycinin), was purified and its posttranslational processing was investigated. Its...
article 2003
document
van der Slot, A.J. (author), Zuurmond, A.M. (author), Bardoel, A.F.J. (author), Wijmenga, C. (author), Pruijs, H.E.H. (author), Sillence, D.O. (author), Brinckmann, J. (author), Abraham, D.J. (author), Black, C.M. (author), Verzijl, N. (author), de Groot, J. (author), Hanemaaijer, R. (author), te Koppele, J.M. (author), Huizinga, T.W.J. (author), Bank, R.A. (author), TNO Preventie en Gezondheid (author)
The hallmark of fibrotic processes is an excessive accumulation of collagen. The deposited collagen shows an increase in pyridinoline cross-links, which are derived from hydroxylated lysine residues within the telopeptides. This change in cross-linking is related to irreversible accumulation of collagen in fibrotic tissues. The increase in...
article 2003
document
Bajramović, J.J. (author), Geutskens, S.B. (author), Bsibsi, M. (author), Boot, M. (author), Hassankhan, R. (author), Verhulst, K.C. (author), van Noort, J.M. (author), Div. Immunological and Infect. Dis., TNO Prevention and Health, PO Box 2215, 2301 CE Leiden, Netherlands (author)
We describe a reverse transcriptase-polymerase chain reaction method for the semiquantitative detection of mRNAs encoding the human heat shock proteins αβ-crystallin, Hsp27, and Hsp60. The method involves the coamplification of cellular mRNA-derived cDNA with a dilution series of a competitor fragment (internal standard), using 1 primer pair...
article 2000
Searched for: subject:"complementary%5C+DNA"
(1 - 20 of 31)

Pages