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van Zanden, J.J. (author), van der Woude, H. (author), Vaessen, J. (author), Usta, M. (author), Wortelboer, H.M. (author), Cnubben, N.H.P. (author), Rietjens, I.M.C.M. (author), TNO Kwaliteit van Leven (author)The present study characterises the effect of phase II metabolism, especially methylation and glucuronidation, of the model flavonoid quercetin on its capacity to inhibit human MRP1 and MRP2 activity in Sf9 inside-out vesicles. The results obtained reveal that 3′-O-methylation does not affect the MRP inhibitory potential of quercetin. However, 4...article 2007
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Smith, R.L. (author), Cohen, S.M. (author), Doull, J. (author), Feron, V.J. (author), Goodman, J.I. (author), Marnett, L.J. (author), Portoghese, P.S. (author), Waddell, W.J. (author), Wagner, B.M. (author), Hall, R.L. (author), Higley, N.A. (author), Lucas-Gavin, C. (author), Adams, T.B. (author), TNO Kwaliteit van Leven (author)A scientifically based guide has been developed to evaluate the safety of naturally occurring mixtures, particularly essential oils, for their intended use as flavor ingredients. The approach relies on the complete chemical characterization of the essential oil and the variability of the composition of the oil in the product intended for...article 2005
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- Zuang, V. (author), Alonso, M.A. (author), Botham, P.A. (author), Eskes, C. (author), Fentem, J. (author), Liebsch, M. (author), van de Sandt, J.J.M. (author), TNO Kwaliteit van Leven (author) article 2005
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Mulder, P.P.J. (author), Zuidema, T. (author), Keestra, N.G.M. (author), Kooij, P.J.F. (author), Elbers, I.J.W. (author), van Rhijn, J.A. (author), TNO Kwaliteit van Leven (author)A method is described for the identification and quantitative determination of 3,5-dinitrosalicylic acid hydrazide (DSH), the marker residue of nifursol metabolites in poultry (turkey, broiler) muscle and liver tissue. The method is based on the acid-catalysed hydrolysis of tissue-bound metabolites to free DSH and in situ derivatisation with 2...article 2005
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Adams, T.B. (author), Cohen, S.M. (author), Doull, J. (author), Feron, V.J. (author), Goodman, J.I. (author), Marnett, L.J. (author), Munro, I.C. (author), Portoghese, P.S. (author), Smith, R.L. (author), Waddell, W.J. (author), Wagner, B.M. (author), TNO Kwaliteit van Leven (author)This publication is the ninth in a series of safety evaluations performed by the Expert Panel of the Flavor and Extract Manufacturers Association (FEMA). In 1993, the Panel initiated a comprehensive program to re-evaluate the safety of more than 1700 GRAS flavoring substances under conditions of intended use. Elements that are fundamental to the...article 2005
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Adams, T.B. (author), Cohen, S.M. (author), Doull, J. (author), Feron, V.J. (author), Goodman, J.I. (author), Marnett, L.J. (author), Munro, I.C. (author), Portoghese, P.S. (author), Smith, R.L. (author), Waddell, W.J. (author), Wagner, B.M. (author), TNO Kwaliteit van Leven (author)This publication is the ninth in a series of safety evaluations performed by the Expert Panel of the Flavor and Extract Manufacturers Association (FEMA). In 1993, the Panel initiated a comprehensive program to re-evaluate the safety of more than 1700 GRAS flavoring substances under conditions of intended use. Elements that are fundamental to the...article 2005
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Adams, T.B. (author), Cohen, S.M. (author), Doull, J. (author), Feron, V.J. (author), Goodman, J.I. (author), Marnett, L.J. (author), Munro, I.C. (author), Portoghese, P.S. (author), Smith, R.L. (author), Waddell, W.J. (author), Wagner, B.M. (author), TNO Kwaliteit van Leven (author)This publication is the eighth in a series of safety evaluations performed by the Expert Panel of the Flavor and Extract Manufacturers Association (FEMA). In 1993, the panel initiated a comprehensive program to re-evaluate the safety of more than 1700 GRAS flavoring substances under conditions of intended use. Elements that are fundamental to...article 2005
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van der Veen, M. (author), Norde, W. (author), Stuart, M.C. (author)The influence of succinylation on lysozyme is studied using circular dichroism, fluorescence spectroscopy, and differential scanning calorimetry. The spectroscopic data reveal that at room temperature the structures of succinylated lysozyme and native lysozyme are similar. However, the calorimetric results show that the thermal stability of...article 2005
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de Brabander, H.F. (author), Poelmans, S. (author), Schilt, R. (author), Stephany, R.W. (author), le Bizec, B. (author), Draisci, R. (author), Sterk, S.S. (author), van Ginkel, L.A. (author), Courtheyn, D. (author), van Hoof, N. (author), Macrì, A. (author), de Wasch, K. (author), TNO Voeding (author)The review summarizes current knowledge on the possible illegal use of the anabolic steroid boldenone. The presence of boldenone and metabolites in different animal species and the possibility of the occurrence of endogenous boldenone and metabolites is assessed, as are the methods of analysis used for detection. Different laboratories in the...article 2004
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Wolken, W.A.M. (author), Tramper, J. (author), van der Werf, M.J. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)The amino acid-catalysed retroaldol condensation previously described for citral has been extended to other α,β -unsaturated aldehydes. In the presence of glycine and an elevated pH, six other α,β-unsaturated aldehydes also underwent retroaldol condensation. Crotonaldehyde, as well as its proposed intermediate, aldol, were converted into...article 2004
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van der Woude, H. (author), Boersma, M.G. (author), Vervoort, J. (author), Rietjens, I.M.C.M. (author), TNO Voeding (author)In this study, the HPLC, UV-vis, LC-MS, and 1H NMR characteristics of 14 different phase II mono- and mixed conjugates of quercetin were determined, providing a useful tool in the identification of quercetin phase II metabolite patterns in various biological systems. Using these data, the phase II metabolism of quercetin by different rat and...article 2004
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Prins Maurits Laboratorium TNO (author), van der Schans, M.J. (author), Polhuijs, M. (author), van Dijk, C. (author), Degenhardt, C.E.A.M. (author), Pleijsier, K. (author), Langenberg, J.P. (author), Benschop, H.P. (author)The utility was explored of a new approach to detect retrospectively exposure to nerve agents by means of conversion of the inhibitor moiety bound to the active site of the enzyme BuChE in plasma with fluoride ions into a phosphofluoridate which is subsequently analyzed by means of gas chromatography (GC). This quantifies ≥0.01% inhibition of...article 2004
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TNO Preventie en Gezondheid (author), Rensen, P.C.N. (author), van Leeuwen, S.H. (author), Sliedregt, L.A.J.M. (author), van Berkel, T.J.C. (author), Biessen, E.A.L. (author)A novel glycolipid has been prepared that contains a cluster glycoside with an unusually high affinity for the asialoglycoprotein receptor (ASGPr) and a bile acid moiety that mediates stable incorporation into lipidic particles. The glycolipid spontaneously associated with low-density lipoproteins (LDL) and high-density lipoproteins (HDL) within...article 2004
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Lakemond, C.M.M. (author), de Jongh, H.H.J. (author), Paques, M. (author), van Vliet, T. (author), Gruppen, H. (author), Voragen, A.G.J. (author), Centre for Protein Technology TNO-WU (author)Formation and structure of glycinin gels were studied in relation to protein conformation for two pH values and three ionic strengths. While at I = 0.03 the gels were found to be fine stranded, gel coarseness increased when the ionic strength was higher. At I = 0.03 finer gel network structures were formed at pH 3.8 than at pH 7.6, whereas for I...article 2003
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Wilderbeek, H.T.A. (author), van der Meer, M.G.M. (author), Jansen, M.A.G. (author), Nelissen, L. (author), Fischer, H.R. (author), van Es, J.J.G.S. (author), Bastiaansen, C.W.M. (author), Lub, J. (author), Broer, D.J. (author), Technisch Physische Dienst TNO - TH (author)Novel phenyl benzoate-based and biphenyl-based liquid crystalline thiol-ene monomers were synthesized and their properties investigated. By varying the bridging unit and spacer length, the type of mesophase can be tuned from the low ordered nematic and smectic A phase in the case of the phenyl benzoate-based monomers, to the highly ordered...article 2003
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Derksen, G.C.H. (author), Naayer, M. (author), van Beek, T.A. (author), Capelle, A. (author), Haaksman, I.K. (author), van Doren, H.A. (author), de Groot, Æ. (author)For the production of a commercially useful dye extract from madder, the glycoside ruberythric acid has to be hydrolysed to the aglycone alizarin which is the main dye component. An intrinsic problem is the simultaneous hydrolysis of the glycoside lucidin pritneveroside to the unwanted mutagenic aglycone lucidin. Madder root was treated with...article 2003
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Soffers, A.E.M.F. (author), Boersma, M.G. (author), Vaes, W.H.J. (author), Vervoort, J. (author), Tyrakowska, B. (author), Hermens, J.L.M. (author), Rietjens, I.M.C.M. (author), Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author)Over the past decades the description of quantitative structure-activity relationships (QSARs) has been undertaken in order to find predictive models and/or mechanistic explanations for chemical as well as biological activities. This includes QSAR studies in toxicology. In an approach beyond the classical QSAR approaches, attempts have been made...article 2001
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Hooijschuur, E.W.J. (author), Kientz, C.E. (author), Hulst, A.G. (author), Brinkman, U.A.T. (author), Prins Maurits Laboratorium TNO (author)On-line coupling of reversed-phase microcolumn liquid chromatography (micro-RPLC) and sulfur-selective flame photometric detection (S-FPD) was studied for the selective and direct determination of thiodiglycol, bis(2- hydroxyethylthio)methane, 1,2-bis(2-hydroxyethylthio)ethane, 1,3-bis(2- hydroxyethylthio)propane, and 1,4-bis(2-hydroxyethylthio...article 2000
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van der Werf, M.J. (author), de Bont, J.A.M. (author), Swarts, H.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Limonene-1,2-epoxide hydrolase from Rhodococcus erythropolis DCL14, an enzyme involved in the limonene metabolism of this microorganism, catalyzes the enantioselective hydrolysis of 1-methylcyclohexene oxide. (1R,2S)-1- Methylcyclohexene oxide was the preferred substrate and it was mainly hydrolyzed to (1S,2S)-1-methylcyclohexane-1,2-diol, while...article 1999
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van den Nieuwendijk, A.M.C.H. (author), Benningshof, J.C.J. (author), Wegmann, V. (author), Bank, R.A. (author), te Koppele, J.M. (author), Brussee, J. (author), van der Gen, A. (author), TNO Preventie en Gezondheid (author)A new synthetic route to reduced collagen crosslinks (LNL and HLNL) is described in this report. It enables an enantioselective synthesis of LNL. HLNL was obtained as a mixture of two diastereoisomers. This method also provides the possibility to introduce radio-labels during the synthesis.article 1999