Searched for: subject%3A%22calcimycin%22
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document
Nelson, G. (author), Kozlova-Zwinderman, O. (author), Collis, A.J. (author), Knight, M.R. (author), Fincham, J.R.S. (author), Stanger, C.P. (author), Renwick, A. (author), Hessing, J.G.M. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Read, N.D. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)
Calcium signalling is little understood in filamentous fungi largely because easy and routine methods for calcium measurement in living hyphae have previously been unavailable. We have developed the recombinant aequorin method for this purpose. High levels of aequorin expression were obtained in Neurospora crassa, Aspergillus niger and...
article 2004
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Ngiam, C. (author), Jeenes, D.J. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Archer, D.B. (author)
Protein disulfide isomerase (PDI) is important in assisting the folding and maturation of secretory proteins in eukaryotes. A gene, pdiA, encoding PDIA was previously isolated from Aspergillus niger, and we report its functional characterization here. Functional analysis of PDIA showed that it catalyzes the refolding of denatured and reduced...
article 2000
document
Emeis, J.J. (author), van den Hoogen, C.M. (author), Diglio, C.A. (author), Gaubius Instituut TNO (author)
The synthesis, storage and regulated secretion (acute release) of tissue-type plasminogen activator (tPA) were studied in cultured rat heart endothelial (RHE) cells. In this study, special attention was paid to the correspondence between tPA metabolism in RHE cells in vitro and tPA metabolism in rats in vivo. RHE cells synthetized tPA, as shown...
article 1998
document
Lupu, C. (author), Goodwin, C.A. (author), Westmuckett, A.D. (author), Emeis, J.J. (author), Scully, M.F. (author), Kakkar, V.V. (author), Lupu, F. (author), Gaubius Instituut TNO (author)
Tissue factor pathway inhibitor (TFPI), the main downregulator of the procoagulant activity of tissue factor·factor VIIa complex, locates in human endothelial cells (EC) in culture as well-defined clusters uniformly distributed both on the cell surface and intracellularly. We here demonstrate by immunofluorescence that TFPI colocalizes in EC...
article 1997
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Gaubius Instituut TNO (author), Schalkwijk, C.G. (author), van der Heijden, M.A.G. (author), Bunt, G. (author), Maas, R. (author), Tertoolen, L.G.J. (author), van Bergen Henegouwen, P.M.P. (author), Verkleij, A.J. (author), van den Bosch, H. (author), Boonstra, J. (author)
The 85 kDa cytosolic phospholipase A2 (cPLA2) preferentially catalyses the hydrolysis of arachidonic acid from the sn-2 position of phospholipids. cPLA2 can be activated by extracellular stimuli such as thrombin, platelet-derived growth factor and epidermal growth factor (EGF). A full activation of cPLA2 requires an increase of intracellular Ca2...
article 1996
Searched for: subject%3A%22calcimycin%22
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