Searched for: subject:"Urinary%5C+Plasminogen%5C+Activator"
(1 - 20 of 49)

Pages

document
Guimarães, A.H.C. (author), Laurens, N. (author), Weijers, E.M. (author), Koolwijk, P. (author), van Hinsbergh, V.W.M. (author), Rijken, D.C. (author), TNO Kwaliteit van Leven (author)
OBJECTIVE - Besides having a key role in fibrinolysis, the plasminogen system has been implicated in cell migration and angiogenesis. A common mechanism is the binding of plasminogen to carboxy-terminal lysine residues in partially degraded fibrin or on cellular surfaces. Here we examined the involvement of thrombin activatable fibrinolysis...
article 2007
document
Rijken, D.C. (author), Barrett-Bergshoeff, M.M. (author), Jie, A.F.H. (author), Criscuoli, M. (author), Sakharov, D.V. (author), Gaubius Instituut TNO (author)
Amediplase (K2tu-PA) is a hybrid plasminogen activator, consisting of the kringle 2 domain of alteplase and the protease domain of urokinase. The objective of this study was to determine the in vitro clot penetration of amediplase in relation to its fibrin binding and to compare the properties with those of alteplase. The clot lysis activity of...
article 2004
document
Plaisier, M. (author), Kapiteijn, K. (author), Koolwijk, P. (author), Fijten, C. (author), Hanemaaijer, R. (author), Grimbergen, J.M. (author), Mulder-Stapel, A. (author), Quax, P.H.A. (author), Helmerhorst, F.M. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
In the endometrium, angiogenesis is a physiological process, whereas in most adult tissues neovascularization is initiated only during tissue repair or pathological conditions. Pericellular proteolysis plays an important role in angiogenesis being required for endothelial cell migration, invasion, and tube formation. We studied the expression of...
article 2004
document
van Hensbergen, Y. (author), Broxterman, H.J. (author), Peters, E. (author), Rana, S. (author), Elderkamp, Y.W. (author), van Hinsbergh, V.W.M. (author), Koolwijk, P. (author), Gaubius Instituut TNO (author)
The aminopeptidase inhibitor bestatin has been shown to have anti-angiogenic effects in a number of model systems. These effects are thought to result from inhibition of CD13 activity. Because tumor angiogenesis can evolve in a fibrin-rich stroma matrix we have studied for the first time the effects of bestatin on microvascular endothelial...
article 2003
document
Lamfers, M.L.M. (author), Grimbergen, J.M. (author), Aalders, M.C. (author), Havenga, M.J. (author), de Vries, M.R. (author), Huisman, L.G.M. (author), van Hinsbergh, V.W.M. (author), Quax, P.H.A. (author), Gaubius instituut TNO (author)
Proteases of the plasminogen activator (PA) and matrix metalloproteinase (MMP) system play an important role in smooth muscle cell (SMC) migration and neointima formation after vascular injury. Inhibition of either PAs or MMPs has previously been shown to result in decreased neointima formation in vivo. To inhibit both protease systems...
article 2002
document
van Hensbergen, Y. (author), Broxterman, H.J. (author), Hanemaaijer, R. (author), Jorna, A.S. (author), van Lent, N.A. (author), Verheul, H.M.W. (author), Pinedo, H.M. (author), Hoekman, K. (author), Gaubius Instituut TNO (author)
Purpose: On the basis of the finding of marked overexpression in angiogenic microvessels, aminopeptidase N/CD13 has recently been suggested to play a prominent role in tumor angiogenesis. A soluble form of CD13 (sCD13) is present in human plasma, but its role in cancer has not been addressed. We hypothesized that sCD13 would be shed by tumor...
article 2002
document
Schulter, V. (author), Koolwijk, P. (author), Peters, E. (author), Frank, S. (author), Hrzenjak, A. (author), Graier, W.F. (author), van Hinsbergh, V.W.M. (author), Kostner, G.M. (author), Gaubius Instituut TNO (author)
Angiostatin, which consists of the kringle I-IV domains of plasminogen and which is secreted into urine, is an efficient inhibitor of angiogenesis and tumor growth. Because N-terminal apolipoprotein(a) [apo(a)] fragments, which also contain several types of kringle IV domains, are found in urine as well, we evaluated the potential angiostatic...
article 2001
document
Koolwijk, P. (author), Sidenius, N. (author), Peters, E. (author), Sier, C.F.M. (author), Hanemaaijer, R. (author), Blasi, F. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Pericellular proteolysis plays an important role in cell migration and the formation of new capillary structures. The plasminogen activator/plasmin and matrix degrading metalloproteinase (MMP) cascades act together in the remodeling of matrix and cell-matrix contacts. Previously we have shown that the formation of capillary structures by human...
article 2001
document
de Maat, M.P.M. (author), Dooijewaard, G. (author), Meijer, P. (author), Binder, B.R. (author), Keber, D. (author), Risberg, B. (author), Kluft, C. (author), Jespersen, J. (author), Gaubius Instituut TNO (author)
In most Westernized societies cardiovascular diseases are the leading cause of death over the age of 45 years and one-quarter of these deaths occur in men below the age of 65 years. The haemostasis system has been identified as an important system in cardiovascular disease (CVD). The European Concerted Action on Prevention from Thrombosis by...
article 2001
document
Quax, P.H.A. (author), Lamfers, M.L.M. (author), Lardenoye, J.H.P. (author), Grimbergen, J.M. (author), de Vries, M.R. (author), Slomp, J. (author), de Ruiter, M.C. (author), Kockx, M.M. (author), Verheijen, J.H. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Background - Smooth muscle cell migration, in addition to proliferation, contributes to a large extent to the neointima formed in humans after balloon angioplasty or bypass surgery. Plasminogen activator/plasmin-mediated proteolysis is an important mediator of this smooth muscle cell migration. Here, we report the construction of a novel hybrid...
article 2001
document
Lamfers, M.L.M. (author), Lardenoye, J.H.P. (author), de Vries, M.R. (author), Aalders, M.C. (author), Engelse, M.A. (author), Grimbergen, J.M. (author), van Hinsbergh, V.W.M. (author), Quax, P.H.A. (author), Gaubius Instituut TNO (author)
Injury-induced neointimal development results from migration and proliferation of vascular smooth muscle cells (SMC). Cell migration requires controlled proteolytic degradation of extracellular matrix surrounding the cell. Plasmin is a major contributor to this process by degrading various matrix proteins directly, or indirectly by activating...
article 2001
document
Sier, C.F.M. (author), Casetta, G. (author), Verheijen, J.H. (author), Tizzani, A. (author), Agape, V. (author), Kos, J. (author), Blasi, F. (author), Hanemaaijer, R. (author), Gaubius Instituut TNO (author)
Matrix metalloproteinases (MMPs) are involved in tumor growth and metastasis, promoting the migration and invasion of cells. In this study, the amount of MMP-2 and MMP-9 activity was measured in urine from superficial bladder carcinoma patients (pTa, pT1) to evaluate their possible diagnostic value. The active and total amount of MMP-2 and MMP-9...
article 2000
document
Braat, E.A.M. (author), Jie, A.F.H. (author), Ronday, H.K. (author), Beekman, B. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
Background - Excessive fibrin deposition within the inflamed joints of rheumatoid arthritis (RA) patients suggests that local fibrinolysis is inefficient, which seems to be in contrast with the observed increased levels of urokinase type plasminogen activator (u-PA). Thrombin-mediated inactivation of single chain u-PA (scu-PA) into an inactive...
article 2000
document
Kroon, M.E. (author), Koolwijk, P. (author), van der Vecht, B. (author), van Hinsbergh, V.W.M. (author), Gaubius Instituut TNO (author)
Hypoxia stimulates angiogenesis, the formation of new blood vessels. This study evaluates the direct effect of hypoxia (1% oxygen) on the angiogenic response of human microvascular endothelial cells (hMVECs) seeded on top of a 3-dimensional fibrin matrix, hMVECs stimulated with fibroblast growth factor-2 (FGF-2) or vascular endothelial growth...
article 2000
document
Sitter, T. (author), Toet, K. (author), Quax, P. (author), Kooistra, T. (author), Gaubius instituut TNO (author)
Background. Human mesothelial cells (HMCs) have an important role in maintaining an adequately functioning fibrinolytic system in the peritoneal cavity by secreting the fibrinolytic enzymes tissue-type and urokinase-type plasminogen activator (t-PA and u-PA), as well as a specific PA inhibitor, PA inhibitor type 1 (PAI-1). In this study, we...
article 1999
document
Lansink, M. (author), Koolwijk, P. (author), van Hinsbergh, V. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)
Angiogenesis, the formation of new capillary blood vessels, is a feature of a variety of pathological processes. To study the effects of a specific group of hormones (all ligands of the steroid/retinoid/thyroid hormone receptor superfamily) on the angiogenic process in humans, we have used a model system in which human microvascular endothelial...
article 1998
document
Braat, E.A.M. (author), Los, P. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
Thrombin cleaves single-chain urokinase-type plasminogen activator (scu- PA) into a virtually inactive two-chain form (tcu-PA/T), a process which may contribute to the maintenance of a fresh blood clot. We have examined the inactivation of scu-PA by thrombin in a plasma milieu to get more insight in the physiological relevance of this phenomenon...
article 1998
document
Quax, P.H.A. (author), Grimbergen, J.M. (author), Lansink, M. (author), Bakker, A.H.F. (author), Blatter, M.C. (author), Belin, D. (author), van Hinsbergh, V.W.M. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)
Urokinase-type plasminogen activator (UPA), particularly when bound to its receptor (UPAR), is thought to play a major role in local proteolytic processes, thus facilitating cell migration as may occur during angiogenesis, neointima and atherosclerotic plaque formation, and tumor cell invasion. To facilitate understanding of the need and...
article 1998
document
Koopman, J.L. (author), Slomp, J. (author), de Bart, A.C.W. (author), Quax, P.H.A. (author), Verheijent, J.H. (author), Gaubius Instituut TNO (author)
The structural and functional properties of the urokinase-type plasminogen activator (u-PA) that are involved in the mitogenic effect of this proteolytic enzyme on human melanoma cells M14 and IF6 and the role of the u-PA receptor (u-PAR) in transducing this signal were analyzed. Native u- PA purified from urine induced a mitogenic response in...
article 1998
document
van Kesteren, P.J.M. (author), Kooistra, T. (author), Lansink, M. (author), van Kamp, G.J. (author), Asscheman, H. (author), Gooren, L.J.G. (author), Emeis, J.J. (author), Vischer, U.M. (author), Stehouwer, C.D.A. (author), Gaubius Instituut TNO (author)
We studied thirteen male-to-female (M→F) and ten female-to-male (F→M) transsexuals who, for four months, received cross-sex treatment with, respectively, ethinylestradiol and cyproterone acetate, and with testosterone esters. We assessed the effects of treatment on plasma levels of tissue-type plasminogen activator (tPA), von Willebrand factor ...
article 1998
Searched for: subject:"Urinary%5C+Plasminogen%5C+Activator"
(1 - 20 of 49)

Pages