Searched for: subject:"Tumor%5C+Cells%2C%5C+Cultured"
(1 - 20 of 38)

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Zaman, G.J.R. (author), Cnubben, N.H.P. (author), van Bladeren, P.J. (author), Evers, R. (author), Borst, P. (author), Instituut CIVO-Toxicologie en Voeding TNO (author)
The multidrug resistance protein MRP has been shown to mediate the transport of glutathione S-conjugates across membranes. In this study we demonstrate that the glutathione S-conjugate of the diuretic drug ethacrynic acid, which is an efficient inhibitor of glutathione S-transferases, is a high-affinity substrate and inhibitor of the glutathione...
article 1996
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Braakhuis, B.J.M. (author), Klaassen, I. (author), van der Leede, B.M. (author), Cloos, J. (author), Brakenhoff, R.H. (author), Copper, M.P. (author), Teerlink, T. (author), Hendriks, H.F.J. (author), van der Saag, P.T. (author), Snow, G.B. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
Retinoids can reverse potentially premalignant lesions and prevent second primary tumours in patients with head and neck squamous cell carcinoma (HNSCC). Furthermore, it has been reported that acquired resistance to all-trans retinoic acid (RA) in leukaemia is associated with decreased plasma peak levels, probably the result of enhanced retinoid...
article 1997
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Kaptein, A. (author), Roodenburg, L. (author), Princen, H.M.G. (author), Gaubius Institute TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
Addition of sodium butyrate to the culture medium of the human hepatoma cell line Hep G2 resulted in a time- and dose-dependent increase in the secretion of apolipoprotein A-I (apo A-I) and apolipoprotein B100 (apo B100). After a 24 h preincubation period, a 2.4- and 2.2-fold increase in the secretion of apo A-I and apo B100 respectively was...
article 1991
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Meijer, C. (author), Timmer, A. (author), de Vries, E.G.E. (author), Groten, J.P. (author), Knol, A. (author), Zwart, N. (author), Dam, W.A. (author), Sleijfer, D.Th. (author), Mulder, N.H. (author)
Cisplatin (CDDP) is an extremely active drug in the treatment of germ- cell tumours. Earlier, we found an unexpected inverse correlation between the total amount of sulfhydryl groups and CDDP sensitivity in a panel of 3 human germ-cell tumour and 3 colon-carcinoma cell lines. Major components of the sulfhydryl groups are glutathione and...
article 2000
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Zhu, D. (author), van Arkel, C. (author), King, C.A. (author), van Meirvenne, S. (author), de Greef, C. (author), Thielemans, K. (author), Radl, J. (author), Stevenson, F.K. (author), TNO Preventie en Gezondheid (author)
The 5T series of multiple myelomas (MM) and Waldenstrsom's macroglobulinaemia-like lymphomas (WM), which developed spontaneously in ageing mice of the C57BL/KaLwRij strain, shows clinical and biological features that closely resemble their corresponding human diseases. In order to compare the patterns of somatic mutation in V(H) genes of mouse...
article 1998
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Garrett, I.R. (author), Dallas, S. (author), Radl, J. (author), Mundy, G.R. (author), TNO Preventie en Gezondheid (author)
Myeloma causes a devastating and unique form of osteolytic bone disease. Although osteoclast activation is responsible for bone destruction, the precise mechanisms by which myeloma cells increase osteoclast activity have not been defined. An animal model of human myeloma bone disease mould help in clarification of these mechanisms. Multiple...
article 1997
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van Zanden, J.J. (author), Geraets, L. (author), Wortelboer, H.M. (author), van Bladeren, P.J. (author), Rietjens, I.M.C.M. (author), Cnubben, N.H.P. (author), TNO Voeding (author)
The objective of this study was to investigate the structural requirements necessary for inhibition of glutathione S-transferase P1-1 (GSTP1-1) and GS-X pump (MRP1 and MRP2) activity by structurally related flavonoids, in GSTP1-1 transfected MCF7 cells (pMTG5). The results reveal that GSTP1-1 activity in MCF7 pMTG5 cells can be inhibited by some...
article 2004
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Braakhuis, B.J.M. (author), Welters, M.J.P. (author), Cloos, J. (author), Pankras, J.E. (author), Smeets, S.J. (author), Fichtinger-Schepman, A.-M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The aim of the present study was to investigate how normal head and neck epithelial cells (NHNEC) respond to cisplatin compared to their neoplastic counterparts with respect to intracellular platinum (Pt) levels and growth inhibition. A colorimetric assay was used to assess growth inhibition after exposure to cisplatin for 72 h. Growth...
article 1999
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Bosma, P.J. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)
In man, the plasminogen activator inhibitor 1 (PAI-1) gene codes for two mRNA species, one of 3.2 kilobases (kb) and the other of 2.4 kb. We report that the protein kinase C activating phorbol ester, phorbol 12-myristate-13-acetate (PMA), causes a different induction of the two PAI-1 mRNA species in the human hepatoma cell line, HepG2. Upon...
article 1991
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Otter, M. (author), van Berkel, T.J.C. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)
In this study, binding and degradation of tissue-type plasminogen activator (t-PA) by the human hepatoma cell line Hep G2 was investigated. Binding at 4°C was time-dependent and reached a maximum after ca. 2 hours. Scatchard analysis of saturation experiments showed about 170,000 high affinity binding sites for t-PA per cell with an apparent Kd...
article 1989
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Rompelberg, C.J.M. (author), Evertz, S.J.C.J. (author), Bruijntjes-Rozier, G.C.D.M. (author), van den Heuvel, P.D. (author), Verhagen, H. (author), TNO Voeding (author)
The influence of in vivo treatment with eugenol on established mutagens was studied to determine whether eugenol has antigenotoxic potential. The effects of eugenol in rats was investigated in the unscheduled DNA synthesis (UDS) assay with established mutagens and the Salmonella typhimurium mutagenicity assay. In addition, the effect of in vitro...
article 1996
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Bajramović, J.J. (author), Geutskens, S.B. (author), Bsibsi, M. (author), Boot, M. (author), Hassankhan, R. (author), Verhulst, K.C. (author), van Noort, J.M. (author), Div. Immunological and Infect. Dis., TNO Prevention and Health, PO Box 2215, 2301 CE Leiden, Netherlands (author)
We describe a reverse transcriptase-polymerase chain reaction method for the semiquantitative detection of mRNAs encoding the human heat shock proteins αβ-crystallin, Hsp27, and Hsp60. The method involves the coamplification of cellular mRNA-derived cDNA with a dilution series of a competitor fragment (internal standard), using 1 primer pair...
article 2000
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Mulder, M. (author), de Wit, E. (author), Havekes, L.M. (author), Gaubius Instituut TNO (author)
It has been suggested that besides the LDL-receptor, hepatocytes possess an apo E or remnant receptor. To evaluate which hepatic lipoprotein receptor is involved in VLDL remnant catabolism, we studied the binding of VLDL remnants to HepG2 cells. Native VLDL was obtained from type IIb hyperlipidemic patients and treated with bovine milk...
article 1991
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Welters, M.J.P. (author), Fichtinger-Schepman, A.M.J. (author), Baan, R.A. (author), Jacobs-Bergmans, A.J. (author), Kegel, A. (author), van der Vijgh, W.J.F. (author), Braakhuis, B.J.M. (author)
Total platinum contents and cisplatin-DNA adduct levels were determined in vivo in xenografted tumour tissues in mice and in vitro in cultured tumour cells of head and neck squamous cell carcinoma (HNSCC), and correlated with sensitivity to cisplatin. In vivo, a panel of five HNSCC tumour lines growing as xenografts in nude mice was used. In...
article 1999
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Mulder, M. (author), Lombardi, P. (author), Jansen, H. (author), van Berkel, T.J.C. (author), Frants, R.R. (author), Havekes, L.M. (author), Gaubius Instituut TNO (author)
It has previously been shown that lipoprotein lipase (LPL) enhances the binding of low density lipoproteins (LDL) and very low density lipoproteins (VLDL) to HepG2 cells and fibroblasts, up to 80-fold. This increase in binding is LDL receptor-independent and is due to a bridging of LPL between extracellular heparan sulfate proteoglycans (HSPG)...
article 1993
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van Iersel, M.L.P.S. (author), Cnubben, N.H.P. (author), Smink, N. (author), Koeman, J.H. (author), van Bladeren, P.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The cyclopentenone prostaglandin A2 (PGA2) is known to inhibit cell proliferation, and metabolism of this compound thus might be important in controlling its ultimate function. The glutathione-related metabolism of PGA2 was therefore investigated both with purified glutathione S-transferase P1-1 (GSTP1-1) and with IGR-39 human melanoma cells....
article 1999
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El-Beltagi, H.M. (author), Martens, A.C.M. (author), Lelieveld, P. (author), Haroun, E.A. (author), Hagenbeek, A. (author), Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author)
Acetyldinaline [CI-994; GOE 5549; PD 123 654; 4-acetylamino-N-(2'- aminophenyl)-benzamide] is the acetylated derivative form of the original compound Dinaline (GOE 1734; PD 104 208). The efficacy and toxicity of Acetyldinaline for remission-induction treatment of leukemia were evaluated and compared with those observed in previous studies of...
article 1993
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Lang, M.S. (author), Oostendorp, R.A.J. (author), Simons, P.J. (author), Boersma, W. (author), Knegt, P. (author), van Ewijk, W. (author)
Both retroviral infections as well as human tumors may cause immunosuppression. One of the factors involved in immunosuppression in patients with squamous cell carcinoma of the head and neck (SCC-HN) is a protein related in the retroviral protein p15E. A conserved, 17-amino acid sequence represents the immunosuppressive epitope of retroviral...
article 1994
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van den Akker, Th.W. (author), Radl, J. (author), Franken-Postma, E. (author), Hagemeijer, A. (author), Gaubius Instituut TNO (author)
Multiple myeloma (MM) and Waldenström's macroglobulinemia-like lymphoma (MW) appear spontaneously in C57BL/KaLwRij mice at a frequency of 0.5% and 0.2%, respectively. They can readily be propagated by intravenous transfer of mainly bone marrow or spleen cells into syngeneic recipients. Previous studies demonstrated that these mouse malignant...
article 1996
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Kypreos, K.E. (author), van Dijk, K.W. (author), van der Zee, A. (author), Havekes, L.M. (author), Zannis, V.I. (author), Gaubius Instituut TNO (author)
Apolipoprotein (apo) E has been implicated in cholesterol and triglyceride homeostasis in humans. At physiological concentration apoE promotes efficient clearance of apoE-containing lipoprotein remnants. However, high apoE plasma levels correlate with high plasma triglyceride levels. We have used adenovirus-mediated gene transfer in apoE...
article 2001
Searched for: subject:"Tumor%5C+Cells%2C%5C+Cultured"
(1 - 20 of 38)

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