Searched for: subject%3A%22Tumor%255C%252BCells%252C%255C%252BCultured%22
(21 - 40 of 41)
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Gaubius Instituut TNO (author), Koopman, J.L. (author), Slomp, J. (author), de Bart, A.C.W. (author), Quax, P.H.A. (author), Verheijent, J.H. (author)The structural and functional properties of the urokinase-type plasminogen activator (u-PA) that are involved in the mitogenic effect of this proteolytic enzyme on human melanoma cells M14 and IF6 and the role of the u-PA receptor (u-PAR) in transducing this signal were analyzed. Native u- PA purified from urine induced a mitogenic response in...article 1998
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Centraal Instituut voor Voedingsonderzoek TNO (author), van Iersel, M.L.P.S. (author), van Lipzig, M.M.H. (author), Rietjens, I.M.C.M. (author), Vervoort, J. (author), van Bladeren, P.J. (author)Using 1H NMR two diastereoisomers of the ethacrynic acid glutathione conjugate (EASG) as well as ethacrynic acid (EA) could be distinguished and quantified individually. Chemically prepared EASG consists of equal amounts of both diastereoisomers. GSTP1-1 stereospecifically catalyzes formation of one of the diastereoisomers (A). The GSTP1-1...article 1998
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Garrett, I.R. (author), Dallas, S. (author), Radl, J. (author), Mundy, G.R. (author), TNO Preventie en Gezondheid (author)Myeloma causes a devastating and unique form of osteolytic bone disease. Although osteoclast activation is responsible for bone destruction, the precise mechanisms by which myeloma cells increase osteoclast activity have not been defined. An animal model of human myeloma bone disease mould help in clarification of these mechanisms. Multiple...article 1997
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Braakhuis, B.J.M. (author), Klaassen, I. (author), van der Leede, B.M. (author), Cloos, J. (author), Brakenhoff, R.H. (author), Copper, M.P. (author), Teerlink, T. (author), Hendriks, H.F.J. (author), van der Saag, P.T. (author), Snow, G.B. (author)Retinoids can reverse potentially premalignant lesions and prevent second primary tumours in patients with head and neck squamous cell carcinoma (HNSCC). Furthermore, it has been reported that acquired resistance to all-trans retinoic acid (RA) in leukaemia is associated with decreased plasma peak levels, probably the result of enhanced retinoid...article 1997
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Gaubius Instituut TNO (author), van Dane-Oorschot, A.A.A.M. (author), Fischer, D.F. (author), Grimbergen, J.M. (author), Klein, B. (author), Zhuang, S.M. (author), Falkenburg, J.H.F. (author), Backendorf, C. (author), Quax, P.H.A. (author), van der Eb, A.J. (author), Noteborn, M.H.M. (author)The chicken anemia virus protein apoptin induces a p53-independent, Bcl- 2-insensitive type of apoptosis in various human tumor cells. Here, we show that, in vitro, apoptin fails to induce programmed cell death in normal lymphoid, dermal, epidermal, endothelial, and smooth-muscle cells. However, when normal cells are transformed they become...article 1997
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Rompelberg, C.J.M. (author), Evertz, S.J.C.J. (author), Bruijntjes-Rozier, G.C.D.M. (author), van den Heuvel, P.D. (author), Verhagen, H. (author), TNO Voeding (author)The influence of in vivo treatment with eugenol on established mutagens was studied to determine whether eugenol has antigenotoxic potential. The effects of eugenol in rats was investigated in the unscheduled DNA synthesis (UDS) assay with established mutagens and the Salmonella typhimurium mutagenicity assay. In addition, the effect of in vitro...article 1996
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van den Akker, Th.W. (author), Radl, J. (author), Franken-Postma, E. (author), Hagemeijer, A. (author), Gaubius Instituut TNO (author)Multiple myeloma (MM) and Waldenström's macroglobulinemia-like lymphoma (MW) appear spontaneously in C57BL/KaLwRij mice at a frequency of 0.5% and 0.2%, respectively. They can readily be propagated by intravenous transfer of mainly bone marrow or spleen cells into syngeneic recipients. Previous studies demonstrated that these mouse malignant...article 1996
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Vergelli, M. (author), Le, H. (author), van Noort, J.M. (author), Dhib-Jalbut, S. (author), McFarland, H. (author), Martin, R. (author), TNO Preventie en Gezondheid (author)CD56 is a member of the neural cell adhesion molecule family expressed on cells of the central nervous system and also on NK cells. Previous studies suggest the involvement of CD56 in effector-to-target cell conjugation mediated by NK cells. It was shown recently that CD56 is also expressed by subpopulations of CD8+ and CD4+ T cells. The present...article 1996
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van Iersel, M.L.P.S. (author), Ploemen, J.P.H.T.M. (author), Struik, I. (author), van Amersfoort, C. (author), Keyzer, A.E. (author), Schefferlie, J.G. (author), van Bladeren, P.J. (author), TNO Voeding (author)The glutathione S-transferase (GST) activity towards 1-chloro-2,4-dinitrobenzene in intact human IGR-39 melanoma cells was determined by the quantification by HPLC-analysis of the excreted glutathione (GSH) conjugate (S-(2,4-dinitrophenyl)glutathione; DNPSG). The major GST subunit expressed in these melanoma cells is the π-class GST subunit P1....article 1996
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Instituut CIVO-Toxicologie en Voeding TNO (author), Zaman, G.J.R. (author), Cnubben, N.H.P. (author), van Bladeren, P.J. (author), Evers, R. (author), Borst, P. (author)The multidrug resistance protein MRP has been shown to mediate the transport of glutathione S-conjugates across membranes. In this study we demonstrate that the glutathione S-conjugate of the diuretic drug ethacrynic acid, which is an efficient inhibitor of glutathione S-transferases, is a high-affinity substrate and inhibitor of the glutathione...article 1996
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de Bart, A.C.W. (author), Quax, P.H.A. (author), Löwik, C.W.G.M. (author), Verheijen, J.H. (author), TNO Preventie en Gezondheid (author)Plasmin-mediated extracellular proteolysis has been implicated in the degradation of bone in normal and pathological conditions. Normal and malignant osteoblasts can produce both tissue-type plasminogen activator (t- PA) and urokinase-type plasminogen activator (u-PA). We have used the osteosarcoma cell line MG63 to address the question of...article 1995
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Lang, M.S. (author), Oostendorp, R.A.J. (author), Simons, P.J. (author), Boersma, W. (author), Knegt, P. (author), van Ewijk, W. (author)Both retroviral infections as well as human tumors may cause immunosuppression. One of the factors involved in immunosuppression in patients with squamous cell carcinoma of the head and neck (SCC-HN) is a protein related in the retroviral protein p15E. A conserved, 17-amino acid sequence represents the immunosuppressive epitope of retroviral...article 1994
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El-Beltagi, H.M. (author), Martens, A.C.M. (author), Lelieveld, P. (author), Haroun, E.A. (author), Hagenbeek, A. (author), Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author)Acetyldinaline [CI-994; GOE 5549; PD 123 654; 4-acetylamino-N-(2'- aminophenyl)-benzamide] is the acetylated derivative form of the original compound Dinaline (GOE 1734; PD 104 208). The efficacy and toxicity of Acetyldinaline for remission-induction treatment of leukemia were evaluated and compared with those observed in previous studies of...article 1993
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Gaubius Instituut TNO (author), Mulder, M. (author), Lombardi, P. (author), Jansen, H. (author), van Berkel, T.J.C. (author), Frants, R.R. (author), Havekes, L.M. (author)It has previously been shown that lipoprotein lipase (LPL) enhances the binding of low density lipoproteins (LDL) and very low density lipoproteins (VLDL) to HepG2 cells and fibroblasts, up to 80-fold. This increase in binding is LDL receptor-independent and is due to a bridging of LPL between extracellular heparan sulfate proteoglycans (HSPG)...article 1993
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Kaptein, A. (author), Roodenburg, L. (author), Princen, H.M.G. (author), Gaubius Institute TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author)Addition of sodium butyrate to the culture medium of the human hepatoma cell line Hep G2 resulted in a time- and dose-dependent increase in the secretion of apolipoprotein A-I (apo A-I) and apolipoprotein B100 (apo B100). After a 24 h preincubation period, a 2.4- and 2.2-fold increase in the secretion of apo A-I and apo B100 respectively was...article 1991
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Mulder, M. (author), de Wit, E. (author), Havekes, L.M. (author), Gaubius Instituut TNO (author)It has been suggested that besides the LDL-receptor, hepatocytes possess an apo E or remnant receptor. To evaluate which hepatic lipoprotein receptor is involved in VLDL remnant catabolism, we studied the binding of VLDL remnants to HepG2 cells. Native VLDL was obtained from type IIb hyperlipidemic patients and treated with bovine milk...article 1991
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Rijken, D.C. (author), Groeneveld, E. (author), Gaubius Instituut TNO (author)Recent studies suggest that plasminogen activators not only hydrolyse a specific arginine-valine bond in plasminogen, but may also cleave other proteins such as fibronectin. We studied the substrate specificity, particularly the preference for arginyl over lysyl peptide bonds, of tissue-type plasminogen activator (t-PA) as well as of two-chain...article 1991
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Gaubius Instituut TNO (author), Bosma, P.J. (author), Kooistra, T. (author)In man, the plasminogen activator inhibitor 1 (PAI-1) gene codes for two mRNA species, one of 3.2 kilobases (kb) and the other of 2.4 kb. We report that the protein kinase C activating phorbol ester, phorbol 12-myristate-13-acetate (PMA), causes a different induction of the two PAI-1 mRNA species in the human hepatoma cell line, HepG2. Upon...article 1991
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Quax, P.H.A. (author), van Leeuwen, R.T.J. (author), Verspaget, H.W. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)In 22 human tumor cell lines the regulation of production of plasminogen activators urokinase (u-PA) and tissue-type (u-PA) and their inhibitors PAI-1 and PAI-2 was studied. These four components may determine the net plasminogen activator activity, which is often associated with tumor development and metastatic processes. The amount of specific...article 1990
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Otter, M. (author), van Berkel, T.J.C. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)In this study, binding and degradation of tissue-type plasminogen activator (t-PA) by the human hepatoma cell line Hep G2 was investigated. Binding at 4°C was time-dependent and reached a maximum after ca. 2 hours. Scatchard analysis of saturation experiments showed about 170,000 high affinity binding sites for t-PA per cell with an apparent Kd...article 1989
Searched for: subject%3A%22Tumor%255C%252BCells%252C%255C%252BCultured%22
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