Searched for: subject:"Tumor%5C%2BCells%2C%5C%2BCultured"
(1 - 7 of 7)
document
Heikkilä, P. (author), Teronen, O. (author), Hirn, M.Y. (author), Sorsa, T. (author), Tervahartiala, T. (author), Salo, T. (author), Konttinen, Y.T. (author), Halttunen, T. (author), Moilanen, M. (author), Hanemaaijer, R. (author), Laitinen, M. (author)
Background. Bisphosphonates reduce the bone metastasis formation and angiogenesis but the exact molecular mechanisms involved are unclear. Progelatinase A (proMMP-2; 78 KDa) is activated up during the tumor spread and metastasis by a cell surface-associated matrix metalloproteinase (membrane-type matrix metalloproteinase [MT1-MMP] or MMP-14)....
article 2003
document
Kypreos, K.E. (author), van Dijk, K.W. (author), van der Zee, A. (author), Havekes, L.M. (author), Zannis, V.I. (author), Gaubius Instituut TNO (author)
Apolipoprotein (apo) E has been implicated in cholesterol and triglyceride homeostasis in humans. At physiological concentration apoE promotes efficient clearance of apoE-containing lipoprotein remnants. However, high apoE plasma levels correlate with high plasma triglyceride levels. We have used adenovirus-mediated gene transfer in apoE...
article 2001
document
Bajramović, J.J. (author), Geutskens, S.B. (author), Bsibsi, M. (author), Boot, M. (author), Hassankhan, R. (author), Verhulst, K.C. (author), van Noort, J.M. (author), Div. Immunological and Infect. Dis., TNO Prevention and Health, PO Box 2215, 2301 CE Leiden, Netherlands (author)
We describe a reverse transcriptase-polymerase chain reaction method for the semiquantitative detection of mRNAs encoding the human heat shock proteins αβ-crystallin, Hsp27, and Hsp60. The method involves the coamplification of cellular mRNA-derived cDNA with a dilution series of a competitor fragment (internal standard), using 1 primer pair...
article 2000
document
Arts, J. (author), Grimbergen, J. (author), Toet, K. (author), Kooistra, T. (author), Gaubius instituut TNO (author)
We have characterized the regulation of plasminogen activator inhibitor- 1 (PAI-1) gene expression by phorbol 12-myristate 13-acetate (PMA), serum, and interleukin-1α (IL-1α) in the human hepatoma cell line HepG2. PMA, serum, and IL-1α induced a rapid and transient 28-fold (PMA), 9-fold (serum), and 23-fold (IL-1α) increase in PAI-1 mRNA,...
article 1999
document
Kaptein, A. (author), Roodenburg, L. (author), Princen, H.M.G. (author), Gaubius Institute TNO Instituut voor verouderings- en vaatziekten onderzoek TNO (author)
Addition of sodium butyrate to the culture medium of the human hepatoma cell line Hep G2 resulted in a time- and dose-dependent increase in the secretion of apolipoprotein A-I (apo A-I) and apolipoprotein B100 (apo B100). After a 24 h preincubation period, a 2.4- and 2.2-fold increase in the secretion of apo A-I and apo B100 respectively was...
article 1991
document
Bosma, P.J. (author), Kooistra, T. (author), Gaubius Instituut TNO (author)
In man, the plasminogen activator inhibitor 1 (PAI-1) gene codes for two mRNA species, one of 3.2 kilobases (kb) and the other of 2.4 kb. We report that the protein kinase C activating phorbol ester, phorbol 12-myristate-13-acetate (PMA), causes a different induction of the two PAI-1 mRNA species in the human hepatoma cell line, HepG2. Upon...
article 1991
document
Quax, P.H.A. (author), van Leeuwen, R.T.J. (author), Verspaget, H.W. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)
In 22 human tumor cell lines the regulation of production of plasminogen activators urokinase (u-PA) and tissue-type (u-PA) and their inhibitors PAI-1 and PAI-2 was studied. These four components may determine the net plasminogen activator activity, which is often associated with tumor development and metastatic processes. The amount of specific...
article 1990
Searched for: subject:"Tumor%5C%2BCells%2C%5C%2BCultured"
(1 - 7 of 7)