Searched for: subject%3A%22Tumor%255C%252BCells%252C%255C%252BCultured%22
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Rompelberg, C.J.M. (author), Evertz, S.J.C.J. (author), Bruijntjes-Rozier, G.C.D.M. (author), van den Heuvel, P.D. (author), Verhagen, H. (author), TNO Voeding (author)
The influence of in vivo treatment with eugenol on established mutagens was studied to determine whether eugenol has antigenotoxic potential. The effects of eugenol in rats was investigated in the unscheduled DNA synthesis (UDS) assay with established mutagens and the Salmonella typhimurium mutagenicity assay. In addition, the effect of in vitro...
article 1996
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van den Akker, Th.W. (author), Radl, J. (author), Franken-Postma, E. (author), Hagemeijer, A. (author), Gaubius Instituut TNO (author)
Multiple myeloma (MM) and Waldenström's macroglobulinemia-like lymphoma (MW) appear spontaneously in C57BL/KaLwRij mice at a frequency of 0.5% and 0.2%, respectively. They can readily be propagated by intravenous transfer of mainly bone marrow or spleen cells into syngeneic recipients. Previous studies demonstrated that these mouse malignant...
article 1996
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Vergelli, M. (author), Le, H. (author), van Noort, J.M. (author), Dhib-Jalbut, S. (author), McFarland, H. (author), Martin, R. (author), TNO Preventie en Gezondheid (author)
CD56 is a member of the neural cell adhesion molecule family expressed on cells of the central nervous system and also on NK cells. Previous studies suggest the involvement of CD56 in effector-to-target cell conjugation mediated by NK cells. It was shown recently that CD56 is also expressed by subpopulations of CD8+ and CD4+ T cells. The present...
article 1996
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van Iersel, M.L.P.S. (author), Ploemen, J.P.H.T.M. (author), Struik, I. (author), van Amersfoort, C. (author), Keyzer, A.E. (author), Schefferlie, J.G. (author), van Bladeren, P.J. (author), TNO Voeding (author)
The glutathione S-transferase (GST) activity towards 1-chloro-2,4-dinitrobenzene in intact human IGR-39 melanoma cells was determined by the quantification by HPLC-analysis of the excreted glutathione (GSH) conjugate (S-(2,4-dinitrophenyl)glutathione; DNPSG). The major GST subunit expressed in these melanoma cells is the π-class GST subunit P1....
article 1996
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Instituut CIVO-Toxicologie en Voeding TNO (author), Zaman, G.J.R. (author), Cnubben, N.H.P. (author), van Bladeren, P.J. (author), Evers, R. (author), Borst, P. (author)
The multidrug resistance protein MRP has been shown to mediate the transport of glutathione S-conjugates across membranes. In this study we demonstrate that the glutathione S-conjugate of the diuretic drug ethacrynic acid, which is an efficient inhibitor of glutathione S-transferases, is a high-affinity substrate and inhibitor of the glutathione...
article 1996
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Garrett, I.R. (author), Dallas, S. (author), Radl, J. (author), Mundy, G.R. (author), TNO Preventie en Gezondheid (author)
Myeloma causes a devastating and unique form of osteolytic bone disease. Although osteoclast activation is responsible for bone destruction, the precise mechanisms by which myeloma cells increase osteoclast activity have not been defined. An animal model of human myeloma bone disease mould help in clarification of these mechanisms. Multiple...
article 1997
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Braakhuis, B.J.M. (author), Klaassen, I. (author), van der Leede, B.M. (author), Cloos, J. (author), Brakenhoff, R.H. (author), Copper, M.P. (author), Teerlink, T. (author), Hendriks, H.F.J. (author), van der Saag, P.T. (author), Snow, G.B. (author)
Retinoids can reverse potentially premalignant lesions and prevent second primary tumours in patients with head and neck squamous cell carcinoma (HNSCC). Furthermore, it has been reported that acquired resistance to all-trans retinoic acid (RA) in leukaemia is associated with decreased plasma peak levels, probably the result of enhanced retinoid...
article 1997
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Gaubius Instituut TNO (author), van Dane-Oorschot, A.A.A.M. (author), Fischer, D.F. (author), Grimbergen, J.M. (author), Klein, B. (author), Zhuang, S.M. (author), Falkenburg, J.H.F. (author), Backendorf, C. (author), Quax, P.H.A. (author), van der Eb, A.J. (author), Noteborn, M.H.M. (author)
The chicken anemia virus protein apoptin induces a p53-independent, Bcl- 2-insensitive type of apoptosis in various human tumor cells. Here, we show that, in vitro, apoptin fails to induce programmed cell death in normal lymphoid, dermal, epidermal, endothelial, and smooth-muscle cells. However, when normal cells are transformed they become...
article 1997
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Zhu, D. (author), van Arkel, C. (author), King, C.A. (author), van Meirvenne, S. (author), de Greef, C. (author), Thielemans, K. (author), Radl, J. (author), Stevenson, F.K. (author), TNO Preventie en Gezondheid (author)
The 5T series of multiple myelomas (MM) and Waldenstrsom's macroglobulinaemia-like lymphomas (WM), which developed spontaneously in ageing mice of the C57BL/KaLwRij strain, shows clinical and biological features that closely resemble their corresponding human diseases. In order to compare the patterns of somatic mutation in V(H) genes of mouse...
article 1998
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Gaubius Instituut TNO (author), Vu-Dac, N. (author), Gervois, P. (author), Torra, I.P. (author), Fruchart, J.C. (author), Kosykh, V. (author), Kooistra, T. (author), Princen, H.M.G. (author), Dallongeville, J. (author), Staels, B. (author)
Hypertriglyceridemia is a metabolic complication of retinoid therapy. In this study, we analyzed whether retinoids increase the expression of apo C- III, an antagonist of plasma triglyceride catabolism. In men, isotretinoin treatment (80 mg/d; 5 d) resulted in elevated plasma apo C-III, but not apo E concentrations. In human hepatoma HepG2 cells...
article 1998
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Gaubius Instituut TNO (author), Koopman, J.L. (author), Slomp, J. (author), de Bart, A.C.W. (author), Quax, P.H.A. (author), Verheijent, J.H. (author)
The structural and functional properties of the urokinase-type plasminogen activator (u-PA) that are involved in the mitogenic effect of this proteolytic enzyme on human melanoma cells M14 and IF6 and the role of the u-PA receptor (u-PAR) in transducing this signal were analyzed. Native u- PA purified from urine induced a mitogenic response in...
article 1998
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Centraal Instituut voor Voedingsonderzoek TNO (author), van Iersel, M.L.P.S. (author), van Lipzig, M.M.H. (author), Rietjens, I.M.C.M. (author), Vervoort, J. (author), van Bladeren, P.J. (author)
Using 1H NMR two diastereoisomers of the ethacrynic acid glutathione conjugate (EASG) as well as ethacrynic acid (EA) could be distinguished and quantified individually. Chemically prepared EASG consists of equal amounts of both diastereoisomers. GSTP1-1 stereospecifically catalyzes formation of one of the diastereoisomers (A). The GSTP1-1...
article 1998
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Braakhuis, B.J.M. (author), Welters, M.J.P. (author), Cloos, J. (author), Pankras, J.E. (author), Smeets, S.J. (author), Fichtinger-Schepman, A.-M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The aim of the present study was to investigate how normal head and neck epithelial cells (NHNEC) respond to cisplatin compared to their neoplastic counterparts with respect to intracellular platinum (Pt) levels and growth inhibition. A colorimetric assay was used to assess growth inhibition after exposure to cisplatin for 72 h. Growth...
article 1999
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van Iersel, M.L.P.S. (author), Cnubben, N.H.P. (author), Smink, N. (author), Koeman, J.H. (author), van Bladeren, P.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
The cyclopentenone prostaglandin A2 (PGA2) is known to inhibit cell proliferation, and metabolism of this compound thus might be important in controlling its ultimate function. The glutathione-related metabolism of PGA2 was therefore investigated both with purified glutathione S-transferase P1-1 (GSTP1-1) and with IGR-39 human melanoma cells....
article 1999
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Arts, J. (author), Grimbergen, J. (author), Toet, K. (author), Kooistra, T. (author), Gaubius instituut TNO (author)
We have characterized the regulation of plasminogen activator inhibitor- 1 (PAI-1) gene expression by phorbol 12-myristate 13-acetate (PMA), serum, and interleukin-1α (IL-1α) in the human hepatoma cell line HepG2. PMA, serum, and IL-1α induced a rapid and transient 28-fold (PMA), 9-fold (serum), and 23-fold (IL-1α) increase in PAI-1 mRNA,...
article 1999
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Turnbull, D. (author), Frankos, V.H. (author), Leeman, W.R. (author), Jonker, D. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)
To test for potential estrogenic activity of plant stanols and plant stanol esters, two short-term tests were performed. These were the E-screen test, which measures a substance's ability to induce proliferation of estrogen-responsive human breast adenocarcinoma (MCF-7) cells in culture, and an in vivo test, which measures uterotrophic activity...
article 1999
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Welters, M.J.P. (author), Fichtinger-Schepman, A.M.J. (author), Baan, R.A. (author), Jacobs-Bergmans, A.J. (author), Kegel, A. (author), van der Vijgh, W.J.F. (author), Braakhuis, B.J.M. (author)
Total platinum contents and cisplatin-DNA adduct levels were determined in vivo in xenografted tumour tissues in mice and in vitro in cultured tumour cells of head and neck squamous cell carcinoma (HNSCC), and correlated with sensitivity to cisplatin. In vivo, a panel of five HNSCC tumour lines growing as xenografts in nude mice was used. In...
article 1999
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Meijer, C. (author), Timmer, A. (author), de Vries, E.G.E. (author), Groten, J.P. (author), Knol, A. (author), Zwart, N. (author), Dam, W.A. (author), Sleijfer, D.Th. (author), Mulder, N.H. (author)
Cisplatin (CDDP) is an extremely active drug in the treatment of germ- cell tumours. Earlier, we found an unexpected inverse correlation between the total amount of sulfhydryl groups and CDDP sensitivity in a panel of 3 human germ-cell tumour and 3 colon-carcinoma cell lines. Major components of the sulfhydryl groups are glutathione and...
article 2000
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Bajramović, J.J. (author), Geutskens, S.B. (author), Bsibsi, M. (author), Boot, M. (author), Hassankhan, R. (author), Verhulst, K.C. (author), van Noort, J.M. (author), Div. Immunological and Infect. Dis., TNO Prevention and Health, PO Box 2215, 2301 CE Leiden, Netherlands (author)
We describe a reverse transcriptase-polymerase chain reaction method for the semiquantitative detection of mRNAs encoding the human heat shock proteins αβ-crystallin, Hsp27, and Hsp60. The method involves the coamplification of cellular mRNA-derived cDNA with a dilution series of a competitor fragment (internal standard), using 1 primer pair...
article 2000
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Geutskens, S.B. (author), van der Eb, M.M. (author), Plomp, A.C. (author), Jonges, L.E. (author), Cramer, S.J. (author), Ensink, N.G. (author), Kuppen, P.J.K. (author), Hoeben, R.C. (author)
The host-immune response against adenoviruses forms a major obstacle for their use as gene therapy vectors for treatment of genetic defects. None the less, they are the preferred vectors for in vivo gene transfer in experimental gene therapy protocols for cancer. In this article we demonstrate the antitumor efficacy of adenovirus-mediated...
article 2000
Searched for: subject%3A%22Tumor%255C%252BCells%252C%255C%252BCultured%22
(1 - 20 of 27)

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