- document
-
te Biesebeke, R. (author), Boussier, A. (author), van Biezen, N. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), TNO Kwaliteit van Leven (author)Filamentous growth of Aspergillus oryzae on solid cereal substrates involves secretion of substrate converting enzymes and a solid substrate specific polarised hyphal growth phenotype. To identify proteins produced under these specific conditions, the extracts of A. oryzae grown on wheat-based media were analysed using N-terminal sequence...article 2006
- document
-
Peighambardoust, S.H. (author), van der Goot, A.J. (author), Boom, R.M. (author), Hamer, R.J. (author), TNO Kwaliteit van Leven (author)The Z-blade mixing behaviour of zero-developed (ZD) doughs from the flours of two wheat cultivars of different gluten strength was compared to that of conventionally mixed dough made from the same flours. In farinograph experiments, use of ZD dough led to shorter development time (with less energy requirement), less stability time, and...article 2006
- document
-
van den Heuvel, E.G.H.M. (author), Wils, D. (author), Pasman, W.J. (author), Saniez, M.H. (author), Kardinaal, A.F.M. (author), TNO Kwaliteit van Leven (author)Background: It is well documented that fermentation of carbohydrates that escape digestion exert several effects supposed to be beneficial for (colonic) health, including an increase in stool volume, a shorter intestinal transit time, production of short chain fatty acids and a decrease of colonic pH (Kritchevsky 1988). NUTRIOSE®FB is a dextrin...article 2005
- document
-
Peighambardoust, S.H. (author), van der Goot, A.J. (author), Hamer, R.J. (author), Boom, R.M. (author)Gluten plays a key role in determining the end-use quality of wheat flour. The important role of the highly aggregated fraction of gluten, glutenin macropolymer (GMP), in dough properties opens up new possibilities for revealing underlying mechanisms related to dough processing. Using a new shear cell and advanced methodology to study the GMP,...article 2005
- document
-
te Biesebeke, R. (author), van Biezen, N. (author), de Vos, W.M. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), TNO Kwaliteit van Leven (author)Solid-state fermentation (SSF) with Aspergillus oryzae results in high levels of secreted protein. However, control mechanisms of gene expression in SSF have been only poorly studied. In this study we show that both glucoamylase (glaB) and protease (alpA, nptB) genes are highly expressed during surface cultivation on wheat-based solid medium,...article 2005
- document
-
te Biesebeke, R. (author), Record, E. (author), van Biezen, N. (author), Heerikhuisen, M. (author), Franken, A. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), TNO Kwaliteit van Leven (author)To study the relation between the number of hyphal tips and protein secretion during growth on a solid substrate, we have constructed two mutant strains of Aspergillus oryzae with increased hyphal branching. We have analysed hydrolytic enzyme activities during growth on wheat kernels (WK) of A. oryzae strains carrying the disrupted allele of the...article 2005
- document
-
Don, C. (author), Lookhart, G. (author), Naeem, H. (author), MacRitchie, F. (author), Hamer, R.J. (author), TNO Voeding (author)Genetic and environmental factors that affect the formation of glutenin macropolymer (GMP) particles were investigated by growing Lance 5+10, Lance 2+12, Warigal 5+10 and Warigal 2+12 lines under widely differing temperature regimes in greenhouse conditions. The GMP characteristics and mixing properties of flour extracted from mature wheat...article 2005
- document
-
te Biesebeke, R. (author), Levin, A. (author), Sagt, C. (author), Bartels, J. (author), Goosen, T. (author), Ram, A. (author), van den Hondel, C. (author), Punt, P. (author), TNO Kwaliteit van Leven (author)Aspergillus oryzae requires polarized growth for colonization of solid substrates, and this growth phenotype differs from that seen in liquid medium. Various experimental approaches were used to identify genes that are differentially expressed when A. oryzae is grown on wheat kernels and in a wheat-based liquid medium. Hybridization of A. oryzae...article 2005
- document
-
TNO Kwaliteit van Leven TNO Voeding (author), Peretz, C. (author), de Pater, N. (author), de Monchy, J. (author), Oostenbrink, J. (author), Heederik, D. (author)In this article, dust and wheat-allergen exposure were assessed among bakers, flour millers, and bakery-ingredient producers, and the risk for sensitization was studied. About 520 inhalable dust and wheat-allergen measurements were made among 270 Dutch workers. The effect of exposure to both inhalable dust and wheat allergens on sensitization...article 2005
- document
-
Avantaggiato, G. (author), Havenaar, R. (author), Visconti, A. (author), TNO Voeding (author)In vitro screening of 14 adsorbent materials, including some commercial products used to detoxify Fusarium-mycotoxins, were tested in the pH range of 3-8 for deoxynivalenol (DON)- and nivalenol (NIV)-binding ability. Only activated carbon showed to be effective with binding capacities of 35.1 μmol and 8.8 μmol DON and NIV/g adsorbent,...article 2004
- document
-
Peighambardoust, S.H. (author), van der Goot, A.J. (author), Hamer, R.J. (author), Boom, R.M. (author), TNO Kwaliteit van Leven (author)This article introduces a new method that uses a shearing device to study the effect of simple shear on the overall properties of pasta-like products made from commercial wheat gluten-starch (GS) blends. The shear-processed GS samples had a lower cooking loss (CL) and a higher swelling index (SI) than unprocessed materials, suggesting the...article 2004
- document
-
Sigoillot, C. (author), Record, E. (author), Belle, V. (author), Robert, J.L. (author), Levasseur, A. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Fournel, A. (author), Sigoillot, J.C. (author), Asther, M. (author), TNO Voeding (author)Three laccases, a natural form and two recombinant forms obtained from two different expression hosts, were characterized and compared for paper pulp bleaching. Laccase from Pycnoporus cinnabarinus, a well known lignolytic fungus, was selected as a reference for this study. The corresponding recombinant laccases were produced in Aspergillus...article 2004
- document
-
Vente-Spreeuwenberg, M.A.M. (author), Verdonk, J.M.A.J. (author), Koninkx, J.F.J.G. (author), Beynen, A.C. (author), Verstegen, M.W.A. (author), TNO Animal Nutrition (author)Two separate experiments, but using the same diets, were designed to study whether the addition to the diet of protein hydrolysates or crystalline glutamine (gln) affect small intestinal integrity (experiment 1) and growth performance (experiment 2). It was hypothesized that dietary supplementation of hydrolysed proteins would increase the...article 2004
- document
-
Wijnands, M.V.W. (author), van Erk, M.J. (author), Doornbos, R.P. (author), Krul, C.A.M. (author), Woutersen, R.A. (author)The effects of different dietary compounds on the formation of aberrant crypt foci (ACF) and colorectal tumours and on the expression of a selection of genes were studied in rats. Azoxymethane-treated male F344 rats were fed either a control diet or a diet containing 10% wheat bran (WB), 0.2% curcumin (CUR), 4% rutin (RUT) or 0.04% benzyl...article 2004
- document
-
Boeriu, C.G. (author), Oudgenoeg, G. (author), Spekking, W.T.J. (author), Berendsen, L.B.J.M. (author), Vancon, L. (author), Boumans, H. (author), Gruppen, H. (author), van Berkel, W.J.H. (author), Laane, C. (author), Voragen, A.G.J. (author), TNO Voeding (author)Heterologous conjugates of wheat arabinoxylan and β-casein were prepared via enzymatic cross-linking, using sequential addition of the arabinoxylan to a mixture of β-casein, peroxidase, and hydrogen peroxide. The maximal formation of adducts between the β-casein and the feruloylated arabinoxylan was reached at a protein-to-arabinoxylan ratio of...article 2004
- document
-
van den Heuvel, E.G.H.M. (author), Wils, D. (author), Pasman, W.J. (author), Bakker, M. (author), Saniez, M.-H. (author), Kardinaal, A.F.M. (author), TNO Voeding (author)Objective: To determine the tolerance of increasing dosages of an incompletely hydrolysed and/or incompletely absorbed food dextrin coming from wheat starch, NUTRIOSE®FB, at daily levels of 10 and 15 g up to 60 and 80 g, respectively. Design: A randomized, double-blind, multiple dose, placebo-controlled, combined crossover and parallel trial....article 2004
- document
-
Wang, M. (author), Oudgenoeg, G. (author), van Vliet, T. (author), Hamer, R.J. (author)In a previous study, we have shown that water unextractable solids (WUS) interfere with gluten formation and affect the quality of the resulting gluten. In this study we aim to explain how WUS can affect the process of gluten formation. To this end, WUS were modified with NaOH, xylanase, horseradish peroxidase (HRP) and hydrogen peroxide (H2O2)....article 2003
- document
-
Record, E. (author), Asther, M. (author), Sigoillot, C. (author), Pagès, S. (author), Punt, P.J. (author), Delattre, M. (author), Haon, M. (author), van den Hondel, C.A.M.J.J. (author), Sigoillot, J.C. (author), Lesage-Meessen, L. (author), Asther, M. (author), TNO Voeding (author)A well-known industrial fungus for enzyme production, Aspergillus niger, was selected to produce the feruloyl esterase FAEA by homologous overexpression for pulp bleaching application. The gpd gene promoter was used to drive FAEA expression. Changing the nature and concentration of the carbon source nature (maltose to glucose; from 2.5 to 60 g l...article 2003
- document
-
Don, C. (author), Lichtendonk, W.J. (author), Plijter, J.J. (author), Hamer, R.J. (author), TNO Voeding (author)Clear correlations exist for glutenin macropolymer (GMP) quantity and rheological properties vs. wheat quality and dough rheological properties, but real insight in understanding these links is still missing. The observation that GMP consists of glutenin particles opens up new possibilities to reveal the underlying mechanism linking glutenin...article 2003
- document
-
Avantaggiato, G. (author), Havenaar, R. (author), Visconti, A. (author), TNO Voeding (author)A novel approach is presented herein to study the intestinal absorption of mycotoxins by using a laboratory model that mimics the metabolic processes of the gastrointestinal (GI) tract of healthy pigs. This model was used to evaluate the small-intestinal absorption of zearalenone from contaminated wheat (4.1 mg/kg) and the effectiveness of...article 2003