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TNO Preventie en Gezondheid (author), Hetzel, C. (author), Janssen, R. (author), Ely, S.J. (author), Kristensen, N.M. (author), Bunting, K. (author), Cooper, J.B. (author), Lamb, J.R. (author), Young, D.B. (author), Thole, J.E.R. (author)We have developed a novel epitope delivery system based on the insertion of peptides within a permissive loop of a bacterial superoxide dismutase molecule. This system allowed high-level expression of heterologous peptides in two mycobacterial vaccine strains, Mycobacterium bovis bacille Calmette- Guerin (BCG) and Mycobacterium vaccae. The...article 1998
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TNO Preventie en Gezondheid (author), Geluk, A. (author), Taneja, V. (author), van Meijgaarden, K.E. (author), Zanelli, E. (author), Abou-Zeid, C. (author), Thole, J.E.R. (author), de Vries, R.R.P. (author), David, C.S. (author), Ottenhoff, T.H.M. (author)T helper 1 cells play a major role in protective immunity against mycobacterial pathogens. Since the antigen (Ag) specificity of CD4+ human T cells is strongly controlled by HLA class II polymorphism, the immunogenic potential of candidate Ags needs to be defined in the context of HLA polymorphism. We have taken advantage of class II-deficient ...article 1998
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Laman, J.D. (author), van Meurs, M. (author), de Smet, B.J.G.L. (author), Schoneveld, A. (author), TNO Preventie en Gezondheid (author)The abundant presence of macrophages and activated T cells in atherosclerotic plaques suggests an active involvement of the immune system in this cardiovascular disease. However, insight into cellular interactions inducing relevant effector functions is still limited. Here, Jon Laman and colleagues propose that local interactions between CD40...article 1997
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Gaubius Instituut TNO (author), van Dane-Oorschot, A.A.A.M. (author), Fischer, D.F. (author), Grimbergen, J.M. (author), Klein, B. (author), Zhuang, S.M. (author), Falkenburg, J.H.F. (author), Backendorf, C. (author), Quax, P.H.A. (author), van der Eb, A.J. (author), Noteborn, M.H.M. (author)The chicken anemia virus protein apoptin induces a p53-independent, Bcl- 2-insensitive type of apoptosis in various human tumor cells. Here, we show that, in vitro, apoptin fails to induce programmed cell death in normal lymphoid, dermal, epidermal, endothelial, and smooth-muscle cells. However, when normal cells are transformed they become...article 1997
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Medisch Biologisch Laboratorium TNO (author), Allaerts, W. (author), Jeucken, P.H.M. (author), Debets, R. (author), Hoefakker, S. (author), Claassen, E. (author), Drexhage, H.A. (author)The population of folliculo-stellate (FS) cells of the rat anterior pituitary has been shown to be ultrastructurally and immunohistochemically heterogeneous. Based on the overlap of ultrastructural characteristics, the localization in the anterior pituitary and the co-expression within the same cel of the S-100 protein (a marker for FS cells)...article 1997
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Nijhuis, E.W.P. (author), Oostervink, F. (author), Hinloopen, B. (author), Rozing, J. (author), Nagelkerken, L. (author), Gaubius Instituut TNO (author)Peripheral blood mononuclear cells (PBMC) from 40 consecutive patients entering a screening program on cognitive impairment were studied in vitro with respect to their sensitivity to dexamethasone (DEX). Phytohemagglutinin-induced proliferation by PBMC from patients with senile dementia of the Alzheimer type (SDAT) was less sensitive to the...article 1996
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Dolhain, R.J.E.M. (author), ter Haar, N.T. (author), Hoefakker, S. (author), Tak, P.P. (author), de Ley, M. (author), Claassen, E. (author), Breedveld, F.C. (author), Miltenburg, A.M.M. (author), TNO Preventie en Gezondheid (author)Data concerning the presence of T-cell-derived cytokines in the rheumatic joint are conflicting, challenging the hypothesis that rheumatoid arthritis (RA) is a T-cell-mediated disease. In this study synovial tissue specimens of 11 patients with RA and eight patients with osteoarthritis (OA) were stained for interferon-gamma IFN-γ) and its...article 1996
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van Noort, J.M. (author), TNO Preventie en Gezondheid (author)The pathogenesis of multiple sclerosis (MS), the major neurological disease of young adults in the Western world, is still poorly understood, and no effective therapy to block MS is available as yet. The clinical symptoms of MS result from inflammatory damage to the insulating myelin sheath of axons in the CNS and - at later stages - to axons...article 1996
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de Jong, E.C. (author), Spanhaak, S. (author), Martens, B.P.M. (author), Kapsenberg, M.L. (author), Penninks, A.H. (author), Wierenga, E.A. (author), TNO Voeding (author)Background: Increasing evidence indicates a prominent role of allergen-specific TH2 cells, with high IL-4 and IL-5 production and low interferon-γ production, in the regulation of IgE and eosinophil production in allergic disorders. However, most studies have concentrated on T cells reactive with inhalation allergens, whereas little is known...article 1996
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Laman, J.D. (author), Claassen, E. (author), Noelle, R.J. (author), TNO Preventie en Gezondheid (author)Initially, a role for the interaction between CD40, expressed on B cells, and gp39 (CD40L), expressed on activated T cells, has been defined in humoral immunity. CD40-CD40L interaction is an essential signal for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching. CD40-CD40L interaction is...article 1996
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Vergelli, M. (author), Le, H. (author), van Noort, J.M. (author), Dhib-Jalbut, S. (author), McFarland, H. (author), Martin, R. (author), TNO Preventie en Gezondheid (author)CD56 is a member of the neural cell adhesion molecule family expressed on cells of the central nervous system and also on NK cells. Previous studies suggest the involvement of CD56 in effector-to-target cell conjugation mediated by NK cells. It was shown recently that CD56 is also expressed by subpopulations of CD8+ and CD4+ T cells. The present...article 1996
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Henwood, J. (author), van Eggermond, M.C.J.A. (author), Boxel van-Dezaire, A.H.H. (author), Schipper, R. (author), den Hoedt, M. (author), Peijnenburg, A. (author), Sanal, Ö. (author), Ersoy, F. (author), Rijkers, G.T. (author), Zegers, B.J.M. (author), Vossen, J.M. (author), van Tol, M.J.D. (author), van den Elsen, P.J. (author), TNO Preventie en Gezondheid (author)In this study, we have investigated the impact of deficient MHC class II expression on the use of TCRBV6 and TCRBJ gene elements, and on the pattern of amino acid incorporation exhibited in the N1-D-N2 segments of the third complementarity-determining region (CDR3) of these TCRBV6 rearrangements. To this end, we have analyzed circulating T cells...article 1996
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Remarque, E.J. (author), Nijhuis, E.W.P. (author), Hinloopen, B. (author), Nagelkerken, L. (author), van der Velde, E.A. (author), Ligthart, G.J. (author), Instituut voor Experimentele Gerontologie TNO (author)To investigate the effects of the altered composition of the helper T cell compartment in ageing on the humoral response to influenza vaccine, we investigated correlations between helper T cell subsets and anti-influenza antibody responses in 23 JUNIEUR healthy young and 41 SENIEUR healthy elderly subjects. Naive helper T cell numbers (CD4...article 1996
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Offermans, M.T.C. (author), Struyk, L. (author), de Geus, B. (author), Breedveld, F.C. (author), van den Elsen, P.J. (author), Rozing, J. (author), TNO Preventie en Gezondheid (author)In order to define the extent of T cell heterogeneity and clonality, unique DNA sequences in the junctional region in rearranged T cell receptor (TcR) genes can be studied. For this purpose we have adapted a non-denaturing nucleic acid gel electrophoresis procedure to detect TcR junctional diversity. Detection of junctional diversity is based...article 1996
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Gaubius Instituut TNO (author), Gerritse, K. (author), Laman, J.D. (author), Noelle, R.J. (author), Aruffo, A. (author), Ledbetter, J.A. (author), Boersma, W.J.A. (author), Claassen, E. (author)We investigated the role of CD40-CD40 ligand (CD40L) interactions in multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE). Activated helper T cells expressing CD40L (gp39) surface protein were found in MS patient brain sections, but not in brain tissue sections of normal controls or patients with other neurological diseases....article 1996
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Primatencentrum TNO (author), Bottius, E. (author), BenMohamed, L. (author), Brahimi, K. (author), Gras, H. (author), Lepers, J.P. (author), Raharimalala, L. (author), Aikawa, M. (author), Meis, J. (author), Slierendregt, B. (author), Tartar, A. (author), Thomas, A. (author), Druilhe, P. (author)In the search for subunit vaccines that are able to induce the type of sterile, protective immunity achieved by irradiated sporozoites, there is increasing evidence that defense mechanisms directed at the intrahepatic stage and Ags expressed at this stage are critical. We have initiated a systematic search for such molecules and report here the...article 1996
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Instituut voor Toegepaste Radiobiologie en Immunologie TNO (author), Lagadec, P. (author), Reveneau, S. (author), Lejeune, P. (author), Pinard, D. (author), Borman, T. (author), Bauer, J. (author), Jeannin, J.F. (author)Although PROb colonic tumor cells are immunosuppressive, the immunomodulator OM 163 induced the disappearance of macroscopic peritoneal nodules in 50% of rats bearing a peritoneal carcinomatosis (p.c.) induced by PROb cells. When the p.o. developed, the number of T lymphocytes was low and the expression of interferon (IFN)-γ mRNA in tumor...article 1996
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van Noort, J.M. (author), van Sechel, A.C. (author), Bajramovic, J.J. (author), El Quagmiri, M. (author), Polman, C.H. (author), Lassmann, H. (author), Ravid, R. (author), TNO Preventie en Gezondheid (author)The identification of key antigens in human autoimmune diseases is a crucial step towards the development of specific intervention. The autoantigen(s) relevant to multiple sclerosis (MS) probably reside in myelin of the central nervous system, the target of the disease. Here we examine proliferative responses of human peripheral blood T cells to...article 1995
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Dobber, R. (author), Tielemans, M. (author), Nagelkerken, L. (author), TNO Preventie en Gezondheid (author)In the present study we investigated whether age-related changes in the composition and functional properties of murine CD4+ T cells are reflected in vivo by a changed humoral response to influenza vaccine in aged mice. After the primary immunization, the titers of influenza-specific IgM, IgG1, IgG2a, and IgG2b, but not of IgG3 and IgE, were...article 1995
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Nijhuis, E.W.P. (author), Hinloopen, B. (author), van Lier, R.A.W. (author), Nagelkerken, L. (author), TNO Preventie en Gezondheid (author)Human CD45RA+ ('naive') and CD45RO+ ('memory') CD4+ T cells were compared with respect to their sensitivity to dexamethasone (DEX). In three different activation pathways, i.e. (i) immobilized anti-CD3, (ii) immobilized anti-CD3 plus soluble anti-CD28 and (iii) soluble anti-CD2 plus soluble anti-CD28, naive CD4+ T cells appeared more sensitive...article 1995