Modification of Glutamine and Lysine Residues in Holo and Apo α-Lactalbumin with Microbial Transglutaminase

The molecular structures determine the physical properties of milk proteins and are important for the texture of many dairy-based foods. Bovine α-lactalbumin (α-LA) is a globular 123 amino acid Ca2+ binding milk protein. Modification with microbial Ca2+ independent transglutaminase (MTGase) was used to modify lysines and glutamines in holo and...

Purification and substrate specificity of transglutaminases from blood and Streptoverticillium mobaraense

A procedure for a fast and simple purification of bovine plasma transglutaminase was developed, which resulted in a homogeneous enzyme preparation. Two different procedures were developed for the purification of pig erythrocyte transglutaminase, both of which resulted in partial purification. Both enzymes were used in cross-linking reactions of...

Microbial transglutaminase production by Streptoverticillium mobaraense: Analysis of amino acid metabolism using mass balances

Metabolic flows, especially those of amino acids, were determined and analyzed at different stages of a batch fermentation for microbial transglutaminase production by Streptoverticillium mobaraense. The method is mainly based on mass balances and measurements of amino acids and other metabolites. The measurements included the consumption rate...