Proteomic analysis of human whole and parotid salivas following stimulation by different tastes

Whole and parotid salivas, collected after stimulation with tastants, were analyzed by 2D electrophoresis and mass spectrometry. In whole saliva, the number of proteins affected by taste stimulation increased in the order sweet < umami < bitter < acid. Annexin A1 and calgranulin A, involved in inflammation, were overrepresented after umami,...

Butyrate stimulates tissue-type plasminogen-activator synthesis in cultured human endothelial cells

Incubation of cultured human endothelial cells with 5-mM-dibutyryl cyclic AMP led to an approx. 2-fold increase in tissue-type plasminogen-activator (t-PA) production over a 24 h incubation period. The stimulating effect of dibutyryl cyclic AMP could be explained by the slow liberation of butyrate, as the effect could be reproduced by addition...

Concomitant increase in hepatic triacylglycerol biosynthesis and cytosolic fatty-acid-binding-protein content after feeding rats with a cholestyramine-containing diet

Cholestyramine feeding of rats increased the rate of palmitate and glycerol incorporation into triacylglycerols of isolated hepatocytes. Concomitantly an increase of fatty-acid binding by hepatic cytosolic proteins was observed, which could be attributed to an elevation of the content of the fatty-acid-binding protein (M(r) 12,000). The...

Protein metabolism in the rat cerebral cortex in vivo and in vitro as affected by the acquisition enhancing drug piracetam

The effect of Piracetam on rat cerebral protein metabolism in vivo and in vitro was studied. It was found that the drug stimulates the uptake of labelled leucine by cerebral cortex slices, has no effect on the incorporation of leucine into cerebral protein, neither in slices nor in vivo, but inhibits the breakdown of newly formed protein in...

Selective early activation of a sodium dependent amino acid transport system in stimulated rat lymphocytes

Chemicals/CAS: 2 amino 2 methylpropionic acid, 62-57-7; amino acid, 65072-01-7; concanavalin A, 11028-71-0; sodium, 7440-23-5; Amino Acids; Aminoisobutyric Acids; Concanavalin A, 11028-71-0; Cyclopentanes; Sodium, 7440-23-5