Searched for: subject:"Spectrometry%2C%5C+Mass%2C%5C+Electrospray%5C+Ionization"
(1 - 19 of 19)
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Jeurissen, S.M.F. (author), Punt, A. (author), Delatour, T. (author), Rietjens, I.M.C.M. (author), TNO Kwaliteit van Leven (author)
The effects of a basil extract on the sulfation and concomitant DNA adduct formation of the proximate carcinogen 1′-hydroxyestragole were studied using rat and human liver S9 homogenates and the human hepatoma cell line HepG2. Basil was chosen since it contains the procarcinogen estragole that can be metabolized to 1′-hydroxyestragole by...
article 2008
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Noort, D. (author), van Zuylen, A. (author), Fidder, A. (author), van Ommen, B. (author), Hulst, A.G. (author), TNO Defensie en Veiligheid (author)
Biomonitoring of exposure to the insecticide permethrin is usually performed by analysis of its urinary metabolites 3-phenoxybenzoic acid (3-PBA) or cis/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid (Cl2CA). We are engaged in the development of a methodology to assess the cumulative internal dose of exposure to...
article 2008
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TNO Kwaliteit van Leven (author), Draisma, H.H.M. (author), Reijmers, T.H. (author), Bobeldijk-Pastorova, I. (author), Meulman, J.J. (author), van Estourgie-Burk, G.F. (author), Bartels, M. (author), Ramaker, R. (author), van der Greef, J. (author), Boomsma, D.I. (author), Hankemeier, T. (author)
Differences in genetic background and/or environmental exposure among individuals are expected to give rise to differences in measurable characteristics, or phenotypes. Consequently, genetic resemblance and similarities in environment should manifest as similarities in phenotypes. The metabolome reflects many of the system properties, and is...
article 2008
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Koster, S. (author), Verpoorte, E. (author), TNO Kwaliteit van Leven (author)
This review presents a thorough overview covering the period 1997-2006 of microfluidic chips coupled to mass spectrometry through an electrospray interface. The different types of fabrication processes and materials used to fabricate these chips throughout this period are discussed. Three 'eras' of interfaces are clearly distinguished. The...
article 2007
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Nielen, M.W.F. (author), van Engelen, M.C. (author), Zuiderent, R. (author), Ramaker, R. (author), TNO Kwaliteit van Leven (author)
An emerging trend is recognised in hormone and veterinary drug residue analysis from liquid chromatography tandem mass spectrometry (LC/MS/MS) based screening and confirmation towards accurate mass alternatives such as LC coupled with time-of-flight (TOF), Fourier transform ion cyclotron resonance (FTICR) or Fourier transform orbitrap (FT...
article 2007
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Smither, S.J. (author), Hill, J. (author), van Baar, B.L.M. (author), Hulst, A.G. (author), de Jong, A.L. (author), Titball, R.W. (author), TNO Defensie en Veiligheid (author)
The outer membrane of Gram-negative bacteria contains proteins that might be good targets for vaccines, antimicrobials or detection systems. The identification of surface located proteins using traditional methods is often difficult. Yersinia pestis, the causative agent of plague, was labelled with biotin. Tagged proteins were visualised through...
article 2007
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Coulier, L. (author), Bas, R. (author), Jespersen, S. (author), Verheij, E. (author), van der Werf, M.J. (author), Hankemeier, T. (author), TNO Kwaliteit van Leven TNO Voeding (author)
We have developed an analytical method, consisting of ion-pair liquid chromatography coupled to electrospray ionization mass spectrometry (IP-LC-ESI-MS), for the simultaneous quantitative analysis of several key classes of polar metabolites, like nucleotides, coenzyme A esters, sugar nucleotides, and sugar bisphosphates. The use of the ion-pair...
article 2006
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Sisu, E. (author), Bosker, W.T.E. (author), Norde, W. (author), Slaghek, T.M. (author), Timmermans, J.W. (author), Peter-Katalinić, J. (author), Cohen-Stuart, M.A. (author), Zamfir, A.D. (author), TNO Kwaliteit van Leven (author)
A combined methodology for obtaining at the preparative scale and characterization by nanoelectrospray ionization (nanoESI) quadrupole time-of-flight (QTOF) mass spectrometry (MS) and tandem MS (MS/MS) of linear polysaccharides modified at the reducing end is presented. Two polydisperse maltodextrins (1000 and 3000 Da) and a high molecular...
article 2006
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Carol, J. (author), Gorseling, M.C.J.K. (author), de Jong, C.F. (author), Lingeman, H. (author), Kientz, C.E. (author), van Baar, B.L.M. (author), Irth, H. (author), TNO Defensie en Veiligheid (author)
A multidimensional analytical method for the rapid determination and identification of proteins has been developed. The method is based on the size-exclusion fractionation of protein-containing samples, subsequent on-line trypsin digestion and desalination, and reversed-phase high-performance liquid chromatography-electrospray mass spectrometry...
article 2005
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Stolker, A.A.M. (author), Brinkman, U.A.T. (author), TNO Kwaliteit van Leven (author)
After a brief introduction into the field of veterinary drugs and growth-promoting agents, the most important EU regulations and directives for the inspection of food-producing animals and animal products regarding the residue control of these substances are presented and discussed. Main attention in the review is on the methods of analysis in...
article 2005
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van Baar, B.L.M. (author), Hulst, A.G. (author), de Jong, A.L. (author), Wils, E.R.J. (author), Prins Maurits Laboratorium TNO (author)
In a follow-up of the earlier characterisation of botulinum toxins type A and B (BTxA and BTxB) by mass spectrometry (MS), types C, D, E, and F (BTxC, BTxD, BTxE, BTxF) were now investigated. Botulinum toxins are extremely neurotoxic bacterial toxins, likely to be used as biological warfare agent. Biologically active BTxC, BTxD, BTxE, and BTxF...
article 2004
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van der Woude, H. (author), Boersma, M.G. (author), Vervoort, J. (author), Rietjens, I.M.C.M. (author), TNO Voeding (author)
In this study, the HPLC, UV-vis, LC-MS, and 1H NMR characteristics of 14 different phase II mono- and mixed conjugates of quercetin were determined, providing a useful tool in the identification of quercetin phase II metabolite patterns in various biological systems. Using these data, the phase II metabolism of quercetin by different rat and...
article 2004
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Sengupta, S. (author), Toh, S.A. (author), Sellers, L.A. (author), Skepper, J.N. (author), Koolwijk, P. (author), Leung, H.W. (author), Yeung, H.W. (author), Wong, R.N.S. (author), Sasisekharan, R. (author), Fan, T.P.D. (author)
Background-Ginseng is a commonly used nutraceutical. Intriguingly, existing literature reports both wound-healing and antitumor effects of ginseng extract through opposing activities on the vascular system. To elucidate this perplexity, we merged a chemical fingerprinting approach with a deconstructional study of the effects of pure molecules...
article 2004
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Loke, M.L. (author), Jespersen, S. (author), Vreeken, R. (author), Halling-Sørensen, B. (author), Tjørnelund, J. (author)
This paper describes the development of a HPLC-MS-MS (ESI) method with baseline separation of oxytetracycline, 4-epi-oxytetracycline, α-apo-oxytetracycline and β-apo-oxytetracycline using an XTerra column and an MeOH-MilliQ-water (containing 8 mM formic acid) mobile phase. Limits of quantification for aqueous standards were in the range of 0.004...
article 2003
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Noort, D. (author), Fidder, A. (author), Hulst, A.G. (author), Prins Maurits Laboratorium TNO (author)
article 2003
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van Baar, B.L.M. (author), Hulst, A.G. (author), de Jong, A.L. (author), Wils, E.R.J. (author), Prins Maurits Laboratorium TNO (author)
A method earlier developed for the mass spectrometric (MS) identification of tetanus toxin (TTx) was applied to botulinum toxins type A and B (BTxA and BTxB). Botulinum toxins are extremely neurotoxic bacterial toxins, likely to be used as biological warfare agent. Biologically active BTxA and BTxB are comprised of a protein complex of the...
article 2002
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Fidder, A. (author), Hulst, A.G. (author), Noort, D. (author), de Ruiter, R. (author), van der Schans, M.J. (author), Benschop, H.P. (author), Langenberg, J.P. (author), Prins Maurits Laboratorium TNO (author)
In this paper a novel and general procedure is presented for detection of organophosphate-inhibited human butyrylcholinesterase (HuBuChE), which is based on electrospray tandem mass spectrometric analysis of phosphylated nonapeptides obtained after pepsin digestion of the enzyme. The utility of this method is exemplified by the positive analysis...
article 2002
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van Baar, B.L.M. (author), Hulst, A.G. (author), Roberts, B. (author), Wils, E.R.J. (author), Prins Maurits Laboratorium TNO (author)
A method was developed for the liquid chromatographic-mass spectrometric (LC-MS) identification of extremely neurotoxic toxins. The method combines sample treatment in a safety containment and analysis of detoxified material in a common laboratory facility. The method was applied to the characterization of neat tetanus toxin and subsequent...
article 2002
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Oudgenoeg, G. (author), Dirksen, E. (author), Ingemann, S. (author), Hilhorst, R. (author), Gruppen, H. (author), Boeriu, C.G. (author), Piersma, S.R. (author), van Berkel, W.J.H. (author), Laane, C. (author), Voragen, A.G.J. (author)
Ferulic acid (FA) is an abundantly present phenolic constituent of plant cell walls. Kinetically controlled incubation of FA and the tripeptide Gly-Tyr-Gly (GYG) with horseradish peroxidase and H2O2 yielded a range of new cross-linked products. Two predominant series of hetero-oligomers of FA linked by dehydrogenation to the peptidyl tyrosine...
article 2002
Searched for: subject:"Spectrometry%2C%5C+Mass%2C%5C+Electrospray%5C+Ionization"
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