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Hissink, A.M. (author), Krüse, J. (author), Kulig, B.M. (author), Verwei, M. (author), Muijser, H. (author), Salmon, F. (author), Leenheers, L.H. (author), Owen, D.E. (author), Lammers, J.H.C.M. (author), Freidig, A.P. (author), McKee, R.H. (author), TNO Kwaliteit van Leven (author)As part of a project designed to develop a framework for extrapolating acute central nervous system (CNS) effects of hydrocarbon solvents in animals to humans, experimental studies were conducted in rats and human volunteers in which acute CNS effects were measured and toxicokinetic data were collected. A complex hydrocarbon solvent, white...article 2007
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Ruijssenaars, H.J. (author), Sperling, E.M.G.M. (author), Wiegerinck, P.H.G. (author), Brands, F.T.L. (author), Wery, J. (author), de Bont, J.A.M. (author), TNO Kwaliteit van Leven (author)A steroid 15β-hydroxylating whole-cell solvent tolerant biocatalyst was constructed by expressing the Bacillus megaterium steroid hydroxylase CYP106A2 in the solvent tolerant Pseudomonas putida S12. Testosterone hydroxylation was improved by a factor 16 by co-expressing Fer, a putative Fe-S protein from Bacillus subtilis. In addition, the...article 2007
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Nijkamp, K. (author), Westerhof, R.G.M. (author), Ballerstedt, H. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven (author)A Pseudomonas putida S12 strain was constructed that is able to convert glucose to p-coumarate via the central metabolite l-tyrosine. Efficient production was hampered by product degradation, limited cellular L-tyrosine availability, and formation of the by-product cinnamate via L-phenylalanine. The production host was optimized by inactivation...article 2007
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Verhoef, S. (author), Ruijssenaars, H.J. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven (author)Pseudomonas putida strain S12palB1 was constructed that produces p-hydroxybenzoate from renewable carbon sources via the central metabolite l-tyrosine. P. putida S12palB1 was based on the platform strain P. putida S12TPL3, which has an optimised carbon flux towards l-tyrosine. Phenylalanine ammonia lyase (Pal) was introduced for the conversion...article 2007
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Lammers, J.H.C.M. (author), Emmen, H.H. (author), Muijser, H. (author), Hoogendijk, E.M.G. (author), McKee, R.H. (author), Owen, D.E. (author), Kulig, B.M. (author), TNO Kwaliteit van Leven (author)To evaluate the neurobehavioral effects of hydrocarbon solvents and to establish a working model for extrapolating animal test data to humans, studies were conducted which involved inhalation exposure of rats and humans to white spirit (WS). The specific objectives of these studies were to evaluate the behavioral effects of exposure to WS in...article 2007
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Ballerstedt, H. (author), Volkers, R.J.M. (author), Mars, A.E. (author), Hallsworth, J.E. (author), Santos, V.A.M.D. (author), Puchalka, J. (author), van Duuren, J. (author), Eggink, G. (author), Timmis, K.N. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven (author)Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics and proteomics studies with other P. putida strains, the P. putida KT2440 genomic database serves as standard reference. The utility of KT2440 whole-genome, high-density oligonucleotide microarrays for transcriptomics studies of other Pseudomonas...article 2007
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TNO Kwaliteit van Leven (author), Hertsenberg, S. (author), Brouwer, D. (author), Lurvink, M. (author), Rubingh, C. (author), Rijnders, E. (author), Tielemans, E. (author)Self-assessment of exposure (SAE) refers to any exposure assessment methodology wherein the worker takes an active role in establishing his or her exposure status. The objective of this study was to investigate the reliability and feasibility of SAE approaches among shoe repair workers collecting exposure data over a 3 month period. This study...article 2007
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McKee, R.H. (author), Lammers, J.H.C.M. (author), Hoogendijk, E.M.G. (author), Emmen, H.H. (author), Muijser, H. (author), Barsotti, D.A. (author), Owen, D.E. (author), Kulig, B.M. (author), TNO Kwaliteit van Leven (author)As a preliminary step to evaluating the acute neurobehavioral effects of hydrocarbon solvents and to establish a working model for extrapolating animal test data to humans, joint neurobehavioral/toxicokinetic studies were conducted which involved administering ethanol to rats and volunteers. The specific objectives of the present studies were to...article 2006
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Kemerink, M. (author), van Duren, J.K.J. (author), van Breemen, A.J.J.M. (author), Wildeman, J. (author), Wienk, M.M. (author), Blom, P.W.M. (author), Schoo, H.F.M. (author), Janssen, R.A.J. (author), TNO Industrie en Techniek (author)The morphology of spin-cast films of poly(p-phenylenevinylene) (PPV) derivatives is studied as a function of the substitution pattern of the conjugated backbone. Moreover, the influence of concentration in the casting solution, annealing, the choice of solvent, and the role of defects are addressed. By using a recently developed scanning-probe...article 2005
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Kok, S.J. (author), Hankemeier, T. (author), Schoenmakers, P.J. (author), TNO Kwaliteit van Leven (author)The on-line coupling of comprehensive two-dimensional liquid chromatography (liquid chromatography × size-exclusion chromatography, LC × SEC) and infrared (IR) spectroscopy has been realized by means of an IR flow cell. The system has been assessed by the functional-group analysis of a series of styrene-methylacrylate (SMA) copolymers with...article 2005
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van Wendel de Joode, B. (author), van Hemmen, J.J. (author), Meijster, T. (author), Major, V. (author), London, L. (author), Kromhout, H. (author), TNO Kwaliteit van Leven (author)Valid and reliable semi-quantitative dermal exposure assessment methods for epidemiological research and for occupational hygiene practice, applicable for different chemical agents, are practically nonexistent. The aim of this study was to assess the reliability of a recently developed semi-quantitative dermal exposure assessment method (DREAM)...article 2005
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- Walton, P. (author), Preller, L. (author), TNO Kwaliteit van Leven (author) article 2005
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van Wendel de Joode, B. (author), Tielemans, E. (author), Vermeulen, R. (author), Wegh, H. (author), Kromhout, H. (author), TNO Kwaliteit van Leven (author)Charcoal cloth pads have been used to assess volatile chemicals on the skin in a laboratory setting; however, they have not yet been applied to measure dermal exposure in occupational settings. This study aimed at evaluating whether charcoal pads can be used to assess dermal exposure to benzene and toluene in workers of a petrochemical plant....article 2005
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The solvent-tolerant Pseudomonas putida S12 as host for the production of cinnamic acid from glucoseNijkamp, K. (author), van Luijk, N. (author), de Bont, J.A.M. (author), Wery, J. (author), TNO Kwaliteit van Leven TNO Voeding (author)A Pseudomonas putida S12 strain was constructed that efficiently produced thefine chemical cinnamic acid from glucose or glycerol via the central metabolite phenylalanine. The gene encoding phenylalanine ammonia lyase from the yeast Rhodosporidium toruloides was introduced. Phenylalanine availability was the main bottleneck in cinnamic acid...article 2005
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Brouwer, D.H. (author), de Pater, N.A.J. (author), Zomer, C. (author), Lurvink, M.W.M. (author), van Hemmen, J.J. (author), TNO Kwaliteit van Leven TNO Defensie en Veiligheid (author)The concept of occupational air requirement (OAR), representing the quantity of air required to dilute the vapor concentration in the work environment resulting from 11 product to a concentration below the occupational exposure limit (OEL), was considered to have potential to discriminate between paints that can and cannot be used safely. The...article 2005
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Preller, L. (author), Burstyn, I. (author), de Pater, N. (author), Kromhout, H. (author), TNO Voeding (author)Objectives: To determine which exposure metrics are sufficient to characterize 'peak' inhalation exposure to organic solvents (OS) during spraying operations. Methods: Personal exposure measurements (n = 27; duration 5-159 min) were collected during application of paints, primers, resins and glues in 15 companies. A MiniRAE Photolonization...article 2004
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de Jongh, H.H.J. (author), Kosters, H.A. (author), Kudryashova, E. (author), Meinders, M.B.J. (author), Trofimova, D. (author), Wierenga, P.A. (author), TNO Voeding (author)Using a variety of spectroscopic techniques, a number of molecular functionalities have been studied in relation to the adsorption process of proteins to air-water interfaces. While ellipsometry and drop tensiometry are used to derive information on adsorbed amount and exerted surface pressure, external reflection circular dichroism, infrared,...article 2004
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Wilderbeek, H.T.A. (author), van der Meer, M.G.M. (author), Jansen, M.A.G. (author), Nelissen, L. (author), Fischer, H.R. (author), van Es, J.J.G.S. (author), Bastiaansen, C.W.M. (author), Lub, J. (author), Broer, D.J. (author), Technisch Physische Dienst TNO - TH (author)Novel phenyl benzoate-based and biphenyl-based liquid crystalline thiol-ene monomers were synthesized and their properties investigated. By varying the bridging unit and spacer length, the type of mesophase can be tuned from the low ordered nematic and smectic A phase in the case of the phenyl benzoate-based monomers, to the highly ordered...article 2003
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Kok, S.J. (author), Wold, C.A. (author), Hankemeier, Th. (author), Schoenmakers, P.J. (author)Two commercial liquid chromatography-Fourier-transform infrared spectroscopy interfaces (LC-FTIR), viz. a flow cell and a solvent-elimination interface have been assessed for use in size-exclusion chromatography (SEC) with respect to their chromatographic integrity (i.e. peak asymmetry, chromatographic resolution), quantitative and qualitative...article 2003
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Helmroth, I.E. (author), Dekker, M. (author), Hankemeier, Th. (author)This article describes the simultaneous diffusion of a migrant and a solvent in low density polyethylene (LDPE). The migrant (Irganox 1076) moves out of the slab, while the solvent (isooctane, n-heptane or cyclohexane) moves inwards. Solvent absorption was measured separately by following the increase of the mass of the slab in time. It can be...article 2003