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Wang, Y. (author), Xiao, Y. (author), Zhang, Y. (author), Chai, C. (author), Wei, G. (author), Wei, X. (author), Xu, H. (author), Wang, M. (author), Ouwerkerk, P.B.F. (author), Zhu, Z. (author), TNO Kwaliteit van Leven (author)Monosaccharides transporters play important roles in assimilate supply for sink tissue development. In this study, a new monosaccharide transporter gene OsMST6 was identified from rice (Oryza sativa L.). The predicted OsMST6 protein shows typical features of sugar transporters and shares 79.6% identity with the rice monosaccharide transporter...article 2008
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van der Kaaij, R.M. (author), Yuan, X.L. (author), Franken, A. (author), Ram, A.F.J. (author), Punt, P.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)In the genome sequence of Aspergillus niger CBS 513.88, three genes were identified with high similarity to fungal α-amylases. The protein sequences derived from these genes were different in two ways from all described fungal α-amylases: they were predicted to be glycosylphosphatidylinositol anchored, and some highly conserved amino acids of...article 2007
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van der Kaaij, R.M. (author), Janeček, Š. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)Currently known fungal α-amylases are well-characterized extracellular enzymes that are classified into glycoside hydrolase subfamily GH13_1. This study describes the identification, and phylogenetic and biochemical analysis of novel intracellular fungal α-amylases. The phylogenetic analysis shows that they cluster in the recently identified...article 2007
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Kralj, S. (author), van Geel-Schutten, G.H. (author), Faber, E.J. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Kwaliteit van Leven (author)Glucansucrase or glucosyltransferase (GTF) enzymes of lactic acid bacteria display high sequence similarity but catalyze synthesis of different α-glucans (e.g., dextran, mutan, alternan, and reuteran) from sucrose. The variations in glucosidic linkage specificity observed in products of different glucansucrase enzymes appear to be based on...article 2005
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Damveld, R.A. (author), van Kuyk, P.A. (author), Arentshorst, M. (author), Klis, F.M. (author), van den Hondel, C.A.M.J.J. (author), Ram, A.F.J. (author), TNO Kwaliteit van Leven TNO Voeding (author)1,3-α-d-Glucan is an important component of the cell wall of filamentous fungi. We have identified a family of five 1,3-α-d-glucan synthase-encoding genes in Aspergillus niger. The agsA gene was sequenced and the predicted protein sequence indicated that the overall domain structure of 1,3-α-d-glucan synthases is conserved in fungi. Using RT-PCR...article 2005
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TNO Kwaliteit van Leven TNO Voeding (author), Ozimek, L.K. (author), Euverink, G.J.W. (author), Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D structure [Meng, G. and...article 2005
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Kralj, S. (author), van Geel-Schutten, G.H. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)Lactobacillus reuteri strain 121 uses sucrose for synthesis of a unique, soluble glucan ('reuteran') with mainly α-(1→4) glucosidic linkages. The gene (gtfA) encoding this glucansucrase enzyme had previously been characterized. Here, a detailed biochemical and molecular analysis of the GTFA enzyme is presented. This is believed to be the first...article 2004
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Nelson, G. (author), Kozlova-Zwinderman, O. (author), Collis, A.J. (author), Knight, M.R. (author), Fincham, J.R.S. (author), Stanger, C.P. (author), Renwick, A. (author), Hessing, J.G.M. (author), Punt, P.J. (author), van den Hondel, C.A.M.J.J. (author), Read, N.D. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)Calcium signalling is little understood in filamentous fungi largely because easy and routine methods for calcium measurement in living hyphae have previously been unavailable. We have developed the recombinant aequorin method for this purpose. High levels of aequorin expression were obtained in Neurospora crassa, Aspergillus niger and...article 2004
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TNO Voeding (author), Pouvreau, L. (author), Gruppen, H. (author), van Koningsveld, G.A. (author), van den Broek, L.A.M. (author), Voragen, A.G.J. (author)The gene of the most abundant protease inhibitor in potato cv. Elkana was isolated and sequenced. The deduced amino acid sequence of this gene showed 98% identity with potato serine protease inhibitor (PSPI), a member of the Kunitz family. Therefore, the most abundant protease inhibitor was considered to be one of the isoforms of PSPI. The PSPI...article 2003
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van Baar, B.L.M. (author), Hulst, A.G. (author), de Jong, A.L. (author), Wils, E.R.J. (author), Prins Maurits Laboratorium TNO (author)A method earlier developed for the mass spectrometric (MS) identification of tetanus toxin (TTx) was applied to botulinum toxins type A and B (BTxA and BTxB). Botulinum toxins are extremely neurotoxic bacterial toxins, likely to be used as biological warfare agent. Biologically active BTxA and BTxB are comprised of a protein complex of the...article 2002
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Korthout, H.A.A.J (author), Caspers, M.P.M (author), Kottenhagen, M.J. (author), Helmer, Q. (author), Wang, M. (author)Over the past few years the presence of p53-like proteins in plants was frequently reported, by using the monoclonal antibody Pab240. By means of protein purification and screening a cDNA library, a Pab240 cross-reacting protein and a cDNA clone were isolated from barley. Peptide- and DNA-sequence analysis identified one and the same protein: 2...article 2002
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Posthuma, C.C. (author), Bader, R. (author), Engelmann, R. (author), Postma, P.W. (author), Hengstenberg, W. (author), Pouwels, P.H. (author)Purification of xylulose 5-phosphate phosphoketolase (XpkA), the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene, xpkA, from Lactobacillus pentosus MD363 are described, xpkA encodes a 788-amino-acid protein with a calculated mass of 88,705 Da. Expression of xpkA...article 2002
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Smit, E. (author), Oling, F. (author), Demel, R. (author), Martinez, B. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Lactobacillus acidophilus, like many other bacteria, harbors a surface layer consisting of a protein (SA-protein) of 43 kDa. SA-protein could be readily extracted and crystallized in vitro into large crystalline patches on lipid monolayers with a net negative charge but not on lipids with a net neutral charge. Reconstruction of the S-layer from...article 2001
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Punt, P.J. (author), Seiboth, B. (author), Weenink, X.O. (author), van Zeijl, C. (author), Lenders, M. (author), Konetschny, C. (author), Ram, A.F.J. (author), Montijn, R. (author), Kubicek, C.P. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)DNA fragments containing genetic information for five secretion-related small GTPases of Aspergillus niger (srgA-E) were isolated and identified as members of different Rab/Ypt subfamilies. This isolation and the search for similar sequences in fungal genomic and EST databases showed that, in contrast to Saccharomyces cerevisiae, filamentous...article 2001
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Hijum, S.A.F.T. (author), Bonting, K. (author), van der Maarel, M.J.E.V. (author), Dijkhuizen, L. (author), TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author)Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and...article 2001
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van der Werf, M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A Baeyer-Villiger mono-oxygenase (BVMO), catalysing the NADPH- and oxygen-dependent oxidation of the monocyclic monoterpene ketones 1-hydroxy-2-oxolimonene, dihydrocarvone and menthone, was purified to homogeneity from Rhodococcus erythropolis DCL14. Monocyclic monoterpene ketone mono-oxygenase (MMKMO) is a monomeric enzyme of molecular mass 60...article 2000
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van den Brink, J.M. (author), Punt, P.J. (author), van Gorcom, R.F.M. (author), van den Hondel, C.A.M.J.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)Cytochrome P450 enzyme systems are found throughout nature and are involved in many different, often complex, bioconversions. In the endoplasmic reticulum of the filamentous fungus Aspergillus niger a cytochrome P450 enzyme system is present that is capable of the para-hydroxylation of benzoate. The expression of the two genes encoding the...article 2000
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Chaillou, S. (author), Bor, Y.-C. (author), Batt, C.A. (author), Postma, P.W. (author), Pouwels, P.H. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding D-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could code for the D-xylulose kinase gene (xylB) and another gene (xylT) encoding a protein of 457 amino acids with significant similarity to the D-xylose-H...article 1998
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van den Brink, J.M. (author), van Zeijl, C.M.J. (author), Brons, J.F. (author), van den Hondel, C.A.M.J.J. (author), van Gorcom, R.F.M. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)In this paper, we describe the cloning and molecular characterization of the Aspergillus niger cytochrome P450 reductase (CPR) gene, cprA. Attempts to clone the cprA gene by heterologous hybridization techniques were unsuccessful. Using the polymerase chain reaction (PCR) with degenerate primers based on conserved regions found in cpr genes from...article 1995
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van Eijsden, R.R. (author), Hoedemaeker, F.J. (author), Díaz, C.L. (author), Lugtenberg, B.J.J. (author), de Sylvia Pater, B. (author), Kijne, J.W. (author)As part of a strategy to determine the precise role of pea (Pisum sativum) lectin, Psl, in nodulation of pea by Rhizobium leguminosarum, mutations were introduced into the genetic determinant for pea lectin by site-directed mutagenesis using PCR. Introduction of a specific mutation, N125D, into a central area of the sugar-binding site resulted...article 1992
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