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Agamennone, V. (author), van den Broek, T.J. (author), de Kat Angelino-Bart, A. (author), Hoevenaars, F.P.M. (author), van der Kamp, J.W. (author), Schuren, F.H.J. (author)The development of microbiome-targeted strategies is limited by individual differences in gut microbiome composition and metabolic responses to interventions. In vitro models that can replicate this variation allow us to conduct pre-clinical studies and assess efficacy. This study describes the exposure of 16 individual fecal microbiota samples...article 2023
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Kortman, G.A.M. (author), Dutilh, B.E. (author), Maathuis, A.J.H. (author), Engelke, U.F. (author), Boekhorst, J. (author), Keegan, K.P. (author), Nielsen, F.G.G. (author), Betley, J. (author), Weir, J.C. (author), Kingsbury, Z. (author), Kluijtmans, L.A.J. (author), Swinkels, D.W. (author), Venema, K. (author), Tjalsma, H. (author)Oral iron administration in African children can increase the risk for infections. However, it remains unclear to what extent supplementary iron affects the intestinal microbiome. We here explored the impact of iron preparations on microbial growth and metabolism in the well-controlled TNO's in vitro model of the large intestine (TIM-2). The...article 2016
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Bloemen, J.G. (author), Olde Damink, S.W.M. (author), Venema, K. (author), Buurman, W.A. (author), Jalan, R. (author), Dejong, C.H.C. (author), TNO Kwaliteit van Leven (author)Background & aims: Prebiotics are increasingly used to improve gut integrity. A presumed mechanism of their beneficial action is the synthesis of short chain fatty acids (SCFA: acetate, propionate and butyrate). High systemic concentrations of propionate and butyrate are toxic and can adversely affect the patient. In physiological situations the...article 2010
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Meesters, R.J.W. (author), van Eijk, H.M.H. (author), ten Have, G.A.M. (author), de Graaf, A.A. (author), Venema, K. (author), van Rossum, B.E.J. (author), Deutz, N.E.P. (author), TNO Kwaliteit van Leven (author)A new method involving zinc sulphate deproteinization was developed to study short chain fatty acids (SCFA) production in the colon and subsequent occurrence of SCFA in blood. SCFA were baseline separated in a 30 min cycle using ion-exclusion chromatography and detected by mass spectrometry. Concentrations could be measured down to 10 μM and...article 2007
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TNO Voeding (author), Venema, K. (author), van Nuenen, M.H.M.C. (author), van den Heuvel, E.G. (author), Pool, W. (author), van der Vossen, J.M.B.M. (author)The objective of this study was to compare the in vivo effect of lactulose on faecal parameters with the effect in a dynamic, computer-controlled in vitro model of the proximal large intestine (TIM-2). Faecal samples from 10 human volunteers collected before (non-adapted) and after 1 week of treatment (10 g/day) with lactulose (lactulose-adapted...article 2003