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Inhibition of mannose receptor-mediated-clearance of tissue-type plasminogen activator (t-PA) by dextran: A new explanation for its antithrombotic effect

Noorman, F. (author), Barrett-Bergshoeff, M.M. (author), Bekkers, M. (author), Emeis, J.J. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)

Dextran is used during surgery as a prophylactic agent to prevent deep venous thrombosis. Recently it has been shown that dextran increases t-PA plasma concentrations in patients. As dextran is a potential ligand for the mannose receptor, we studied whether this glucose-polymer would be able to inhibit mannose receptor-mediated clearance of t-PA...

article 1997

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Monoclonal antibodies against the human mannose receptor that inhibit the binding of tissue-type plasminogen activator

Barrett-Bergshoeff, M.M. (author), Moorman, F. (author), Bos, R. (author), Rijken, D.C. (author), Gaubius Instituut TNO (author)

To study the role of the mannose receptor in cellular uptake and degradation of tissue-type plasminogen activator (t-PA), a set of five monoclonal antibodies (Moab) was generated against the mannose receptor isolated from human placental tissue. All Moab specifically recognised the 175 kDa mannose receptor in a crude placenta extract, as shown...

article 1997

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Retinoid (vitamin A) metabolism: A review

Hendriks, H.F.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)

Objective and conclusion: An overview is given of our understanding of the uptake and metabolism of retinoids. The mechanisms underlying retinol uptake by organs and tissues are still unsettled. The retinol-binding proteins CRBP I and CRBP II appear to play an essential role in retinyl ester hydrolysis and formation and in retinoic acid...

article 1996

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Difference in expression of the plasminogen activation system in synovial tissue of patients with rheumatoid arthritis and osteoarthritis

Ronday, H.K. (author), Smits, H.H. (author), van Muijen, G.N.P. (author), Pruszczynski, M.S.M. (author), Dolhain, R.J.E.M. (author), van Langelaan, E.J. (author), Breedveld, F.C. (author), Verheijen, J.H. (author), Gaubius Instituut TNO (author)

Proteolytic joint destruction in inflammatory and non-inflammatory arthropathy is believed to be mediated, at least in part, by the plasminogen activation (PA) system. To further investigate possible involvement of the PA system, we quantified immunoreactive urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (t-PA),...

article 1996

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Lysine-based cluster mannosides that inhibit ligand binding to the human mannose receptor at nanomolar concentration

TNO Preventie en Gezondheid Gaubius Instituut TNO (author), Biessen, E.A.L. (author), Noorman, F. (author), van Teijlingen, M.E. (author), Kuiper, J. (author), Barrett-Bergshoeff, M.M. (author), Bijsterbosch, M.K. (author), Rijken, D.G. (author), van Berkel, T.J.G. (author)

In search of synthetic high affinity ligands for the mannose receptor, we synthesized a series of lysine-based oligomannosides containing two (M2L) to six (M6L5) terminal alpha-D-mannose groups that are connected with the backbone by flexible elongated spacers (16 Å). The synthesized cluster mannosides were all able to displace binding of...

article 1996

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Tumor necrosis factor α induces endothelial galactosyl transferase activity and verocytotoxin receptors. Role of specific tumor necrosis factor receptors and protein kinase C

van de Kar, N.C.A.J. (author), Kooistra, T. (author), Vermeer, M. (author), Lesslauer, W. (author), Monnens, L.A.H. (author), van Hinsbergh, V.W.M. (author), TNO Preventie en Gezondheid (author)

Infections with verocytotoxin (VT) producing Escherichia coil have been strongly implicated in the epidemic form of hemolytic uremic syndrome (HUS). Endothelial damage plays a central role in the pathogenesis of HUS. In vitro studies have shown that VT can damage endothelial cells after interaction with its cellular receptor...

article 1995

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Degradation of tissue-type plasminogen activator by human monocyte- derived macrophages is mediated by the mannose receptor and by the low- density lipoprotein receptor-related protein

Noorman, F. (author), Braat, E.A.M. (author), Rijken, D.C. (author), TNO Preventie en Gezondheid (author)

The balance of tissue-type plasminogen activator (t-PA) production and degradation determines its concentration in blood and tissues. Disturbance of this balance may result in either increased or decreased proteolysis. In the present study, we identified the receptor systems involved in the degradation of t-PA by human monocytes/macrophages in...

article 1995

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Role of the glycosaminoglycan component of thrombomodulin in its acceleration of the inactivation of single-chain urokinase-type plasminogen activator by thrombin

de Munk, G.A.W. (author), Parkinson, J.F. (author), Groeneveld, E. (author), Bang, N.U. (author), Rijken, D.C. (author), IVVO-TNO Gaubius Laboratory, PO Box 430, 2300 AK Leiden, Netherlands Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

Thrombomodulin (TM), a membrane proteoglycan on endothelial cells, binds thrombin in a 1:1 complex, accelerates the protein C activation by thrombin, promotes the thrombin inactivation by antithrombin III and inhibits the procoagulant properties of thrombin. The inactivation of single-chain urokinase-type plasminogen activator (scu-PA) by...

article 1993

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Inactivation of high and low molecular weight single-chain urokinase-type plasminogen activator (pro-urokinase) by thrombin in the presence of thrombomodulin

de Munk, G.A.W. (author), Molinari, A. (author), Rijken, D.C. (author), Gaubius Laboratory Netherlands Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

Chemicals/CAS: amino acid, 65072-01-7; prourokinase, 82657-92-9; thrombin, 9002-04-4; thrombomodulin, 112049-68-0; Enzyme Precursors; Receptors, Cell Surface; Receptors, Thrombin; saruplase, 99149-95-8; Thrombin, EC 3.4.21.5; Urinary Plasminogen Activator, EC 3.4.21.73

article 1993

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Characterization of the interaction both in vitro and in vivo of tissue-type plasminogen activator (t-PA) with rat liver cells. Effects of monoclonal antibodies to t-PA

Otter, M. (author), Kuiper, J. (author), Bos, R. (author), Rijken, D.C. (author), van Berkel, T.J.C. (author), Gaubius Laboratory Instituut voor verouderings- en vaatziekten onderzoek TNO (author)

The interaction of 125I-labelled tissue-type plasminogen activator (125I-t-PA) with freshly isolated rat parenchymal and endothelial liver cells was studied. Binding experiments at 4°C with parenchymal cells and endothelial liver cells indicated the presence of 68000 and 44000 high-affinity t-PA-binding sites, with an apparent K(d) of 3.5 and 4...

article 1992

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Tumor necrosis factor and interleukin-1 induce expression of the verocytotoxin receptor globotriaosylceramide on human endothelial cells: Implications for the pathogenesis of the hemolytic uremic syndrome

Instituut voor verouderings- en vaatziekten onderzoek TNO (author), van de Kar, N.C.A.J. (author), Monnens, L.A.H. (author), Karmali, M.A. (author), van Hinsbergh, V.W.M. (author)

The epidemic form of the hemolytic uremic syndrome (HUS), beginning with an acute gastroenteritis, has been associated with a verocytotoxin-producing Escherichia coli infection. The endothelial cell is believed to play an important role in the pathogenesis of HUS. Endothelial cell damage by verocytotoxin-1 (VT-1) in vitro is potentiated by the...

article 1992

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A sensitive method to detect cell surface receptors using biotinylated growth factors

de Jong, M.O. (author), Rozemuller, H. (author), Visser, J.W. (author), Bauman, J.G. (author)

article 1992

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Mechanisms of tissue-type plasminogen activator (tPA) clearance by the liver

Gaubius Instituut TNO (author), Otter, M. (author), Kuiper, J. (author), Berkel T.J.C. van, (author), Rijken, D.C. (author)

Chemicals/CAS: mannose receptor; Mannose, 31103-86-3; PLAT protein, human; Receptors, Cell Surface; Receptors, Immunologic; Recombinant Proteins; Tissue Plasminogen Activator, EC 3.4.21.68.

article 1992

document

Impact of endothelial activation on fibrinolysis and local proteolysis in tissue repair

Gaubius Instituut TNO (author), van Hinsbergh, V.W.M. (author)

Chemicals/CAS: Cytokines; Plasminogen Activator Inhibitor 1; plasminogen activator, urokinase receptors; Plasminogen, 9001-91-6; Receptors, Cell Surface; Urinary Plasminogen Activator, EC 3.4.21.73.

article 1992

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Acceleration of the thrombin inactivation of single chain urokinase-type plasminogen activator (pro-urokinase) by thrombomodulin

Instituut voor Verouderings- en Vaatziekten Onderzoek TNO (author), de Munk, G.A.W. (author), Groeneveld, E. (author), Rijken, D.C. (author)

The in vitro effects of thrombomodulin on the inactivation of single chain urokinase-type plasminogen activator (scu-PA) by thrombin were investigated by incubating scu-PA with varying concentrations of human thrombin, in both the absence and presence of soluble rabbit thrombomodulin. 50% inactivation of scu-PA occurred in 45 min at 160 ng/ml...

article 1991

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Effects of compactin, mevalonate and low-density lipoprotein on 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and low-density-lipoprotein-receptor activity in the human hepatoma cell line Hep G2

Gaubius instituut TNO (author), Cohen, L.H. (author), Griffioen, M. (author), Havekes, L. (author)

Compactin, an inhibitor of HMG-CoA (3-hydroxy-3-methylglutaryl-CoA) reductase, decreased cholesterol synthesis in intact Hep G2 cells. However, after the inhibitor was washed away, the HMG-CoA-reductase activity determined in the cell homogenate was found to be increased. Also the high-affinity association of LDL (low-density lipoprotein) to Hep...

article 1984

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Low density lipoprotein metabolism by endothelial cells from human umbilical cord arteries and veins

van Hinsbergh, V.W.M. (author), Havekes, L. (author), Emeis, J.J. (author), van Corven, E. (author), Scheffer, M. (author), Gaubius Instituut TNO (author)

Binding and metabolism of low density lipoprotein (LDL) and acetylated LDL were examined in endothelial cells from human umbilical cord arteries and veins. Both high and low affinity LDL interactions were observed. High affinity LDL binding and catabolism were increased five- to sevenfold after preincubation for 18 hours in LPDS containing...

article 1983

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The metabolism in vitro of human low-density lipoprotein by the human hepatoma cell line Hep G2

Gaubius instituut TNO (author), Havekes, L. (author), van Hinsbergh, V. (author), Kempen, H.J. (author), Emeis, J. (author)

The human hepatoma cell line Hep G2 was studied with respect to metabolism of human low-density lipoprotein (LDL). The Hep G2 cells bind, take up and degrade human LDL with a high-affinity saturable and with a low-affinity non-saturable component. The high-affinity binding possesses a K(D) of 25 nM-LDL and a maximal amount of binding of about 70...

article 1983

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Glucagon receptors: Are they present on human blood cells?

van Hinsbergh, V.W.M. (author), Nooijen, W.J. (author), Kempen, H.J.M. (author), Gaubius instituut TNO (author)

Binding of glucagon to and its degradation by human mononuclear leukocytes and erythrocytes was investigated. Labeled glucagon binds to these cells, but can be displaced only by unlabeled glucagon at pharmacologically high concentrations. It is concluded that glucagon binding to human blood cells does not represent receptors of physiological...

article 1982

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Binding of LP(a) to the low density lipoprotein receptor of human fibroblasts

Gaubius Instituut TNO (author), Havekes, L. (author), Vermeer, B.J. (author), Brugman, T. (author), Emeis, J. (author)

Chemicals/CAS: Lipoprotein(a); Lipoproteins; Lipoproteins, LDL; Receptors, Cell Surface; Receptors, LDL

article 1981