Isolation of α-amylase on crosslinked starch

The isolation and purification of α-amylase from an industrial enzyme sample is described using crosslinked starch powder as an affinity adsorbent. The process was studied with regard to the stability of the adsorbent, the stability of the enzyme, and the capacity of the adsorbent for the enzyme. The adsorbent was used in a repeated adsorption...

Improvements in the 32P-postlabelling procedure to quantify bulky aromatic DNA adducts

This contribution describes methodological modifications and improvements that may contribute to inter-assay reproducibility and more accurate adduct quantification for 32P-postlabelling. Firstly, an anion-exchange chromatography procedure was developed to determine the amount of DNA used per assay and to check its purity, in particular to...

Detection of GTP-binding proteins in barley aleurone protoplasts

We report the existence of several families of GTP-binding proteins in barley aleurone protoplasts. Partial purified plasma membrane proteins were separated by SDS-PAGE, transferred to a nitrocellulose filter and incubated with either antisera raised against a highly conserved animal G protein alpha subunit peptide/or Ras protein, or with [alpha...

Translation controls the expression level of a chimaeric reporter gene

Transcriptional and translational fusions between the reading frame of the β-D-glucuronidase gene (gusA) and the 2′ as well as the 1′ promoter of mannopine synthase (mas), a TR locus of Agrobacterium tumefaciens, were made. The expression of these constructs was studied in the transgenic F1 offspring of independent tobacco transformants at the...

Purification and characterization of two lipoxygenase isoenzymes from germinating barley

Two lipoxygenase isoenzymes (linoleate:oxygen oxidoreductase, EC 1.13.11.12) present in the embryo of germinating barley seed have been purified to homogeneity and characterized. Both isoenzymes are monomeric proteins with a molecular mass of approx. 90 kDa and crossreact on Western blots with antibodies raised against pea lipoxygenase. They...

Contaminated Soil '90. 3rd International KfK/TNO Conference on Contaminated Soil. Held at: Karlsruhe, Germany, 10-14 December 1990 (Volume 1 en 2)

Islet cell antibodies, mumps infection and mumps vaccination

Chemicals/CAS: Autoantibodies; islet cell antibody; Mumps Vaccine

The TNO-Thijssen crystallization process and wash-column

A new cooling cryztallization process for industrial purification of organic compounds is developed. The innovating aspects of the process are formed by the unique flow path, based on a co-current transport of crystals and melt, and by the design and operation of the separation and purification section: the wash-column.

Purification and partial characterization of plasminogen activator from human uterine tissue

A procedure was developed for the purification of a plasminogen activator from human uterine tissue. It involves six consecutive steps: extraction of the plasminogen activator from delipidated uterine tissue with 0.3 M potassium acetate buffer, pH 4.2; ammonium sulphate precipitation; zinc chelate-agarose chromatography; n-butyl-agarose...

Mycotoxins

Chemicals/CAS: 3 nitropropionic acid, 504-88-1; aflatoxin B1, 1162-65-8; aflatoxin B2, 7220-81-7; aflatoxin G1, 1165-39-5; aflatoxin G2, 7241-98-7; aflatoxin, 1402-68-2; diacetoxyscirpenol, 2270-40-8; fumagillin, 23110-15-8; gliotoxin, 37273-88-4, 67-99-2; kojic acid, 501-30-4; luteoskyrin, 21884-44-6; ochratoxin, 303-47-9, 37203-43-3;...

Incomplete sedimentation of Rauscher leukaemia virus particles during ultracentrifugation

Use of reagent-inpregnated ('Patho-Tec') test papers in the identification of enterobacteriaceae and similar bacteria

A comparative examination of Patho-Tec reagent-impregnated paper strips and the corresponding classical biochemical tests for the identification of Enterobacteriaceae was made. Patho-Tec papers appeared to be reliable for the cytochrome-oxidase and phenylalanine-deaminase tests. The urease test papers gave false negative reactions in Citrobacter...