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Record, E. (author), Asther, M. (author), Sigoillot, C. (author), Pagès, S. (author), Punt, P.J. (author), Delattre, M. (author), Haon, M. (author), van den Hondel, C.A.M.J.J. (author), Sigoillot, J.C. (author), Lesage-Meessen, L. (author), Asther, M. (author), TNO Voeding (author)A well-known industrial fungus for enzyme production, Aspergillus niger, was selected to produce the feruloyl esterase FAEA by homologous overexpression for pulp bleaching application. The gpd gene promoter was used to drive FAEA expression. Changing the nature and concentration of the carbon source nature (maltose to glucose; from 2.5 to 60 g l...article 2003
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Artola-Garicano, E. (author), Borkent, I. (author), Hermens, J.L.M. (author), Vaes, W.H.J. (author)In the current study, the removal of slowly degradable hydrophobic chemicals in sewage treatment plants (STPs) has been evaluated with emphasis on the combination of free and total concentration data. Free and total concentrations of two polycyclic musks were determined in each compartment of four STPs. The free concentration of the polycyclic...article 2003
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Brink, M. (author), de Goeij, A.F.P.M. (author), Weijenberg, M.P. (author), Roemen, G.M.J.M. (author), Lentjes, M.H.F.M. (author), Pachen, M.M.M. (author), Smits, K.M. (author), de Bruïne, A.P. (author), Goldbohm, R.A. (author), van den Brandt, P.A. (author)Activation of K-ras oncogene has been implicated in colorectal carcinogenesis, being mutated in 30-60% of the adenocarcinomas. In this study, 737 incident colorectal cancer (CRC) patients, originating from 120 852 men and women (55-69 years at baseline) participating in the Netherlands Cohort Study (NLCS), were studied in order to evaluate...article 2003
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Knippels, L.M.J. (author), Penninks, A.H. (author), TNO Voeding (author)The need for widely accepted and validated animal models to test the potential allergenicity and potency of novel (biotechnology-derived) proteins has become an important issue for their safety evaluation.In this article, we summarize the results of the development of an oral sensitization protocol for food proteins in the rat. Young Brown...article 2003
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Artola-Garicano, E. (author), Hermens, J.L.M. (author), Vaes, W.H.J. (author)In the current study, predictions by Simple Treat 3.0, a fate model for organic chemicals in sewage treatment plants (STPs), are compared with actual measurements in three STPs. Two polycyclic musks, Tonalide® (AHTN) and Galaxolide® (HHCB), were used for model evaluation. Results show that Simple Treat 3.0 is able to predict the removal...article 2003
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), van Hijum, S.A.F.T. (author), van der Maarel, M.J.E.C. (author), Dijkhuizen, L. (author)Inulosucrases catalyze transfer of a fructose moiety from sucrose to a water molecule (hydrolysis) or to an acceptor molecule (transferase), yielding inulin. Bacterial inulin production is rare and a biochemical analysis of inulosucrase enzymes has not been reported. Here we report biochemical characteristics of a purified recombinant...article 2003
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Hooijschuur, E.W.J. (author), Kientz, C.E. (author), Brinkman, U.A.T. (author)Today, the determination of chemical warfare agents (CWAs) is an important area of application in analytical chemistry. Chromatographic, capillary electrophoretic and mass spectrometric techniques are primarily used for the identification and quantification of a broad field of classical CWAs in environmental samples and neutralization masses,...article 2002
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Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding (author), Korthout, H.A.A.J (author), Caspers, M.P.M (author), Kottenhagen, M.J. (author), Helmer, Q. (author), Wang, M. (author)Over the past few years the presence of p53-like proteins in plants was frequently reported, by using the monoclonal antibody Pab240. By means of protein purification and screening a cDNA library, a Pab240 cross-reacting protein and a cDNA clone were isolated from barley. Peptide- and DNA-sequence analysis identified one and the same protein: 2...article 2002
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TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO (author), Posthuma, C.C. (author), Bader, R. (author), Engelmann, R. (author), Postma, P.W. (author), Hengstenberg, W. (author), Pouwels, P.H. (author)Purification of xylulose 5-phosphate phosphoketolase (XpkA), the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene, xpkA, from Lactobacillus pentosus MD363 are described, xpkA encodes a 788-amino-acid protein with a calculated mass of 88,705 Da. Expression of xpkA...article 2002
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TNO Voeding (author), Fischer, M. (author), Gruppen, H. (author), Piersma, S.R. (author), Kofod, L.V. (author), Schols, H.A. (author), Voragen, A.G.J. (author)With the purpose of analyzing the size and composition of enzyme-unextractable proteins in differently heat-treated soybean meals, a selection of extractants was screened for their ability to extract these proteins from enzyme-unextractable residues. The largest effects were obtained with urea, urea plus β-mercaptoethanol, and dilute alkali; the...article 2002
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Conesa, A. (author), van den Hondel, C.A.M.J.J. (author), Punt, P.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)To get insight into the limiting factors existing for the efficient production of fungal peroxidase in filamentous fungi, the expression of the Phanerochaete chrysosporium lignin peroxidase H8 (lipA) and manganese peroxidase (MnP) H4 (mnp1) genes in Aspergillus niger has been studied. For this purpose, a protease-deficient A. niger strain and...article 2000
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van der Werf, M.J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)A Baeyer-Villiger mono-oxygenase (BVMO), catalysing the NADPH- and oxygen-dependent oxidation of the monocyclic monoterpene ketones 1-hydroxy-2-oxolimonene, dihydrocarvone and menthone, was purified to homogeneity from Rhodococcus erythropolis DCL14. Monocyclic monoterpene ketone mono-oxygenase (MMKMO) is a monomeric enzyme of molecular mass 60...article 2000
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Gucek, M. (author), Gaspari, M. (author), Walhagen, K. (author), Vreeken, R.J. (author), Verheij, E.R. (author), van der Greef, J. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)The successful coupling of capillary electrochromatography (CEC) to an ion trap mass spectrometer via a nanoelectrospray interface (nESI) is described. Using a conductively coated tip butted to the end of a CEC column, it was possible to obtain a stable spray without any sheath liquid being employed. Selected small peptides were separated with...article 2000
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Energieonderzoek Centrum Nederland (author), Dinkelbach, L. (author), van der Meijden, C.M. (author), Brenneisen, L.M. (author), Strating, S.D. (author), Geleijn, M. (author), Wubbe, R. (author)-report 2000
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Holtman, W.L. (author), Roberts, M.R. (author), Oppedijk, B.J. (author), Testerink, C. (author), van Zeijl, M.J. (author), Wang, M. (author)Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were...article 2000
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Pots, A.M. (author), Gruppen, H. (author), Hessing, M. (author), van Boekel, M.A.J.S. (author), Voragen, A.G.J. (author)Patatin has, so far, been considered a homogeneous group of proteins. A comparison of the isoforms in terms of structural properties or stability has not been reported. A method to obtain various isoform fractions as well as a comparison of the physicochemical properties of these pools is presented. Patatin could be separated in four isoform...article 1999
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Aktypis, A. (author), Kalantzopoulos, G. (author), Huis in't Veld, J.H.J. (author), ten Brink, B. (author), Centraal Instituut voor Voedingsonderzoek TNO (author)ACA-DC 0040 produced an antimicrobial agent, which was named thermophilin T, active against several lactic acid bacteria strains of different species and food spoilage bacteria, such as Clostridium sporogenes C22/10 and Cl. tyrobutyricum NCDO-1754. The crude antimicrobial compound is sensitive to proteolytic enzymes and α-amylase, heat-stable ...article 1998
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Verdoes, D. (author), Arkenbout, G.J. (author), Bruinsma, O.S.L. (author), Koutsoukos, P.G. (author), Ulrich, J. (author)Innovative separation techniques like melt crystallization have the potential to fulfil two important demands, namely: a significant reduction of energy consumption by the chemical industry, and the production of high quality products required by industry. Several industrial applications of melt crystallization have been realized but industry is...article 1997
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Ploemen, J.-P.H.T.M. (author), van Iersel, M.L.P.S. (author), Wormhoudt, L.W. (author), Commandeur, J.N.M. (author), Vermeulen, N.P.E. (author), van Bladeren, P.J. (author)The drug disulfiram (DSF, Antabuse®) has been used in the therapy of alcohol abuse. It is a potent inhibitor of aldehyde dehydrogenase. Its reduced form, diethyldithiocarbamate (DDTC), and further metabolites show similar activities. DSF and DDTC have also been widely used to inhibit mixed-function oxidases. In this study, the reversible...article 1996
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Jespersen, S. (author), Koedam, J.A. (author), Hoogerbrugge, C.M. (author), Tjaden, U.R. (author), van der Greef, J. (author), van den Brande, J.L. (author), TNO Voeding (author)High molecular weight precursors of insulin-like growth factor II (IGF-II) were isolated from Cohn fraction IV of human plasma by ultrafiltration, affinity chromatography and reversed-phase high-performance liquid chromatography. Molecular weight determination by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of two...article 1996