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Streekstra, E.J. (author), Russel, F.G.M. (author), van de Steeg, E. (author), de Wildt, S.N. (author)Drug disposition in children is different compared to adults. Growth and developmental change the processes involved in drug disposition and efficacy, including membrane transporters and drug metabolizing enzymes, but for many of these proteins, the exact changes have not been fully elucidated to date. Quantitative proteomics offers a solution...article 2021
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van Groen, B.D. (author), van de Steeg, E. (author), Mooij, M.G. (author), van Lipzig, M.M.H. (author), de Koning, B.A.E. (author), Verdijk, R.M. (author), Wortelboer, H.M. (author), Gaedigk, R. (author), Bi, C. (author), Leeder, S. (author), van Schaik, R.H.N. (author), van Rosmalen, J. (author), Tibboel, D. (author), Vaes, W.H. (author), de Wildt, S.N. (author)Background Hepatic membrane transporters are involved in the transport of many endogenous and exogenous compounds, including drugs. We aimed to study the relation of age with absolute transporter protein expression in a cohort of 62 mainly fetus and newborn samples. Methods Protein expressions of BCRP, BSEP, GLUT1, MCT1, MDR1, MRP1, MRP2, MRP3,...article 2018
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Wegler, C. (author), Gaugaz, F.Z. (author), Andersson, T.B. (author), Wiśniewski, J.R. (author), Busch, D. (author), Gröer, C. (author), Oswald, S. (author), Norén, A. (author), Weiss, F. (author), Hammer, H.S. (author), Joos, T.O. (author), Poetz, O. (author), Achour, B. (author), Rostami-Hodjegan, A. (author), van de Steeg, E. (author), Wortelboer, H.M. (author), Artursson, P. (author)Many different methods are used for mass-spectrometry-based protein quantification in pharmacokinetics and systems pharmacology. It has not been established to what extent the results from these various methods are comparable. Here, we compared six different mass spectrometry-based proteomics methods by measuring the expression of clinically...article 2017
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- de Marchi, T. (author), Braakman, R.B.H. (author), Stingl, C. (author), van Duijn, M.M. (author), Smid, M. (author), Foekens, J.A. (author), Luider, T.M. (author), Martens, J.W.M. (author), Umar, A. (author) article 2016
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Ramautar, R. (author), Berger, R. (author), van der Greef, J. (author), Hankemeier, T. (author)Metabolomics provides a direct functional read-out of the physiological status of an organism and is in principle ideally suited to describe someone's health status. Whereas only a limited number of small metabolites are used in the clinics, in inborn errors of metabolism an extensive repertoire of metabolites are used as biomarkers. We discuss...article 2013
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Bachmair, E.-M. (author), Bots, M.L. (author), Mennen, L.I. (author), Kelder, T. (author), Evelo, C.T. (author), Horgan, G.W. (author), Ford, I. (author), de Roos, B. (author)Scope: The dietary fatty acid cis9,trans11 conjugated linoleic acid (cis9,trans11 CLA) has been shown to modify the function of endothelial cells, monocytes, and platelets, all of which are involved in the development of atherosclerosis. Potential mechanisms for the platelet effects have not been assessed previously. In this study, we assessed...article 2012
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van Gool, A.J. (author), Hendrickson, R.C. (author)Cerebrospinal fluid (CSF) can be considered the most promising biosample for the discovery and analysis of biomarkers in neuroscience, an area of great medical need. CSF is a body fluid that surrounds the brain and provides a rich pool of biochemical markers, both proteomic and metabolomic, that reflect the state of neurological processes. Such...article 2012
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Rodríguez-Gutiérrez, G. (author), Duthie, G.G. (author), Wood, S. (author), Morrice, P. (author), Nicol, F. (author), Reid, M. (author), Cantlay, L.L. (author), Kelder, T. (author), Horgan, G.W. (author), Fernández-Bolaños Guzmán, J. (author), de Roos, B. (author)Scope: Olive products are rich in phenolic compounds, which are natural antioxidants in vitro. We tested the in vivo effects of alperujo, an olive production by-product, as well as hydroxytyrosol and 3,4-dihydroxyphenylglycol (DHPG) isolated from alperujo, on indices and pathways of oxidative and metabolic stress in a vitamin E-deficient rat...article 2012
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Pellis, L. (author), van Erk, M.J. (author), van Ommen, B. (author), Bakker, G.C.M. (author), Hendriks, H.F.J. (author), Cnubben, N.H.P. (author), Kleemann, R. (author), van Someren, E.P. (author), Bobeldijk, I. (author), Rubingh, C.M. (author), Wopereis, S. (author)We introduce the metabolomics and proteomics based Postprandial Challenge Test (PCT) to quantify the postprandial response of multiple metabolic processes in humans in a standardized manner. The PCT comprised consumption of a standardized 500 ml dairy shake containing respectively 59, 30 and 12 energy percent lipids, carbohydrates and protein....article 2012
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Brul, S. (author), van Beilen, J. (author), Caspers, M. (author), O'Brien, A. (author), de Koster, C. (author), Oomes, S. (author), Smelt, J. (author), Kort, R. (author), ter Beek, A. (author)Bacterial spore formers are prime organisms of concern in the food industry. Spores from the genus Bacillus are extremely stress resistant, most notably exemplified by high thermotolerance. This sometimes allows surviving spores to germinate and grow out to vegetative cells causing food spoilage and possible intoxication. Similar issues though...article 2011
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Wijte, D. (author), van Baar, B.L.M. (author), Heck, A.J.R. (author), Altelaar, A.F.M. (author)To enhance target production from biocatalysts, it is necessary to thoroughly understand the molecular mechanisms involved in production, degradation, and, importantly, adaptation to the required environment. One such bacterium with high potential for biocatalysis is the solvent-tolerant bacteria Pseudomonas putida S12, which, among others, is...article 2011
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Verhoef, S. (author), Ballerstedt, H. (author), Volkers, R.J.M. (author), de Winde, J.H. (author), Ruijssenaars, H.J. (author), TNO Kwaliteit van Leven (author)A transcriptomics and proteomics approach was employed to study the expression changes associated with p-hydroxybenzoate production by the engineered Pseudomonas putida strain S12palB1. To establish p-hydroxyben-zoate production, phenylalanine-tyrosine ammonia lyase (pal/tal) was introduced to connect the tyrosine biosynthetic and p-coumarate...article 2010
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Adav, S.S. (author), Li, A.A. (author), Manavalan, A. (author), Punt, P. (author), Sze, S.K. (author), TNO Kwaliteit van Leven (author)The natural lifestyle of Aspergillus niger made them more effective secretors of hydrolytic proteins and becomes critical when this species were exploited as hosts for the commercial secretion of heterologous proteins. The protein secretion profile of A. niger and its mutant at different pH was explored using iTRAQ-based quantitative proteomics...article 2010
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TNO Kwaliteit van Leven (author), van Ommen, B. (author), Bouwman, J. (author), Dragsted, L.O. (author), Drevon, C.A. (author), Elliott, R. (author), de Groot, P. (author), Kaput, J. (author), Mathers, J.C. (author), Müller, M. (author), Pepping, F. (author), Saito, J. (author), Scalbert, A. (author), Radonjic, M. (author), Rocca-Serra, P. (author), Travis, A. (author), Wopereis, S. (author), Evelo, C.T. (author)The challenge of modern nutrition and health research is to identify food-based strategies promoting life-long optimal health and well-being. This research is complex because it exploits a multitude of bioactive compounds acting on an extensive network of interacting processes. Whereas nutrition research can profit enormously from the revolution...article 2010
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Rosenling, T. (author), Slim, C.L. (author), Christin, C. (author), Coulier, L. (author), Shi, S. (author), Stoop, M.P. (author), Bosman, J. (author), Suits, F. (author), Horvatovich, P.L. (author), Stockhofe-Zurwieden, N. (author), Vreeken, R. (author), Hankemeier, T. (author), van Gool, A.J. (author), Luider, T.M. (author), Bischoff, R. (author), TNO Kwaliteit van Leven (author)To standardize the use of cerebrospinal fluid (CSF) for biomarker research, a set of stability studies have been performed on porcine samples to investigate the influence of common sample handling procedures on proteins, peptides, metabolites and free amino acids. This study focuses at the effect on proteins and peptides, analyzed by applying...article 2009
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Hamer, H.M. (author), Jonkers, D.M.A.E. (author), Loof, A. (author), van Houtvin, S.A.L.W. (author), Troost, F.J. (author), Venema, K. (author), Kodde, A. (author), Koek, G.H. (author), Schipper, R.G. (author), van Heerde, W.L. (author), Brummer, R.J. (author), TNO Kwaliteit van Leven (author)Background: The mucus layer is an important dynamic component of the epithelial barrier. It contains mucin glycoproteins and other compounds secreted by the intestinal epithelium, such as secretory IgA. However, a standardized in vivo sampling technique of mucus in humans is not yet available. Aim: To assess the validity and feasibility of mucin...article 2009
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Geert Heidema, A. (author), Thissen, U. (author), Boer, J.M.A. (author), Bouwman, F.G. (author), Feskens, E.J.M. (author), Mariman, E.C.M. (author), TNO Kwaliteit van Leven (author)In this study, we applied the multivariate statistical tool Partial Least Squares (PLS) to analyze the relative importance of 83 plasma proteins in relation to coronary heart disease (CHD) mortality and the intermediate end points body mass index, HDL-cholesterol and total cholesterol. From a Dutch monitoring project for cardiovascular disease...article 2009
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Molecular characterization of trimellitic anhydride-induced respiratory allergy in Brown Norway ratsKuper, C.F. (author), Heijne, W.H. (author), Dansen, M. (author), Verhoeckx, K.C. (author), Boorsma, A. (author), Radonjic, M. (author), Bruijntjes, J. (author), Stierum, R. (author), Muijser, H. (author), Arts, J.H. (author), TNO Kwaliteit van Leven (author)To contribute to the hazard identification of low molecular weight (LMW) respiratory allergens, respiratory allergy induced by trimellitic anhydride (TMA) was characterized by whole genome analysis of lung tissue and blood proteomics in Brown Norway rats. Dermal sensitization (50% and 25% w/v) with TMA and an inhalation challenge of 15 mg/m(3)...article 2008
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Dihal, A.A. (author), van der Woude, H. (author), Hendriksen, P.J.M. (author), Charif, H. (author), Dekker, L.J. (author), IJsselstijn, L. (author), de Boer, V.C.J. (author), Alink, G.M. (author), Burgers, P.C. (author), Rietjens, I.M.C.M. (author), Woutersen, R.A. (author), Stierum, R.H. (author), TNO Kwaliteit van Leven (author), KvL (author)Quercetin has been shown to act as an anticarcinogen in experimental colorectal cancer (CRC). The aim of the present study was to characterize transcriptome and proteome changes occurring in the distal colon mucosa of rats supplemented with 10 g quercetin/kg diet for 11 wk. Transcriptome data analyzed with Gene Set Enrichment Analysis showed...article 2008
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Gaining Insight into Microbial Physiology in the Large Intestine: A Special Role for Stable Isotopesde Graaf, A.A. (author), Venema, K. (author), TNO Kwaliteit van Leven (author)The importance of the human large intestine for nutrition, health, and disease, is becoming increasingly realized. There are numerous indications of a distinct role for the gut in such important issues as immune disorders and obesity-linked diseases. Research on this long-neglected organ, which is colonized by a myriad of bacteria, is a rapidly...conference paper 2008